Description
Recombinant full-length human DGKI was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NP_004708.1.
Diacylglycerol kinase iota (DGKI) is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases modulate the concentration of intracellular diacylglycerol by phosphorylating, producing phosphatidic acid (PA)(1). PA regulates signaling proteins, such as protein kinases and phosphatases, and can induce cell mitosis through regulation of growth factors (2). DGKI has been shown to overexpress in a variety of cancers and is associated with poor prognosis in colon and gastric cancer (3)