Description
Short Description: Recombinant Mononucleosomes H3S10ph (EPL) consist of a 167 bp of 601 DNA and two molecules each of histones H2A that includes amino acids 1-130 (end)(accession number NP_003503.1), H2B that includes amino acids 1-126 (end)(accession number NP_003509.1), H3.2 that includes amino acids 1-136 (end)(accession number NP_066403.2) with phosphorylation at serine 10, and H4 that includes amino acids 1-103 (end)(accession number NP_003539.1). All of these histones were expressed in E Coli. The molecular weight of histone octamer is ~108 kDa. H3S10ph (Histone H3 phosphoryl Ser10) protein is generated using expressed protein ligation (EPL) technology. Truncated human Histone H3.2 is produced in E Coli and purified using FPLC. The purified protein is subsequently ligated to an N-terminal histone tail peptide containing phosphoryl serine 10 via a native peptide bond