Description
Recombinant 2019-nCoV Spike protein S1 subunit, RBD (E484Q)(319-541) was expressed in CHO cells using a C-terminal his tag. The gene accession number is MN908947
The receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein that recognizes the host ACE2 receptor is a major determinant of viral entry and neutralization, and is the most divergent region (1). Mutation at E484 in the RBD region of the spike protein has been detected in the South African (B.1.351), Brazilian (P.1), and NY variants (B.1.526). Lineage B.1.617, also known as the Indian variant carries E484Q mutation in combination with L452R. Viruses harboring mutations in the spike protein have reduced susceptibility to both monoclonal antibodies and convalescent plasma (2). As these new variants displace the first-wave virus, it is pivotal to evaluate their transmissibility, virulence and their possible tendency to escape antibody neutralization (3)