Description
Full length recombinant human CK1a1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_001892.
CK1alpha1 is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes. CK1alpha1 can regulate Smo cell surface accumulation and activity in response to hedgehog. CK1alpha1 phosphorylate Smo at several sites and phosphorylation-deficient forms of Smo fail to accumulate on the cell surface and are unable to transduce the hedgehog signal (1). CK1alpha1 dynamically associates with the CBM complex on T cell receptor engagement to participate in cytokine production and lymphocyte proliferation. CK1alpha1 can form complex with MDM2 which then regulates the stability of p53 and E2F-1 transcription factors (2)