Description
Recombinant tag-free Bsu DNA polymerase large fragment from Bacillus subtilis was expressed in E Coli cells. The gene accession number is AF008220. The protein accession number is AAC00350.1.
DNA polymerase (DNAP) is an enzyme that catalyzes the DNA molecules and is crucial for DNA replication. Bsu DNA polymerase large fragment is a product of the Bacillus subtilis DNA polymerase I that retains the 5´--> 3´ polymerase activity of DNA polymerase I but lacks the 5´--> 3´ and 3´--> 5´ exonuclease activity (1). Bsu DNA polymerase has a wide range of applications such as primer labelling, second-strand cDNA synthesis, DNA strand displacement synthesis, recombinase polymerase amplification, and single dA tailing (2)