Description
One family of inhibitory axon guidance molecules is the semaphorins, which include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance during neuronal development and after CNS injury. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region.
Human recombinant Semaphorin-3A protein includes amino acids 26 to 771, as well as a C-terminal human IgG FC domain. The protein is detected by rabbit polyclonal anti-Sema3A (N-terminal region), anti-Sema3A (Central region), and anti-Sema3A (C-terminal). The antibodies detect both full length Sema3A/Fc chimera (125kDa) and the cleaved full length Sema3A (95kDa)