Description
Recombinant full-length human PDHK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The PDHK1 gene accession number is NM_002610.
PDHK1 or Pyruvate Dehydrogenase Kinase 1 is a member of the PDHK family that phosphorylate and inactivate the Pyruvate Dehydrogenase (PDH)(1). PDH is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. PDHK1 is an important regulator of PDH in clonal beta-cells and the activity of PDHK1 and PDH are important for efficient metabolic coupling. Maintaining low PDHK1 expression/activity and keeping PDH in a dephosphorylated and active state, is important for beta -cells to achieve the metabolic flux rates necessary for maximal glucose-stimulated insulin secretion (2)