Description
Short Description: Recombinant human Histone H3 trimethyl Lys27 (H3K27me3)(accession number NP_003520.1) was expressed in E Coli and contains two substitutions (cysteine to serine at amino acid 96 and cysteine to alanine at amino acid 110). Recombinant methylated histones are specifically methylated via a chemical alkylation reaction that introduces a methyl lysine analog (MLA). The molecular weight of the protein is 15,285 Daltons. The recombinant histone is >92% pure by SDS-PAGE. Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro