Description
Recombinant human MET (T992I)(956-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_000245.
MET is a proto-oncogene that encodes a transmembrane growth factor receptor which is a heterodimer of two disulphide linked chains of 50kDa (alpha) and 145kDa (beta). MET is widely expressed in the kidney, brain, lung, skin, and embryonic tissue (1). Hepatocyte growth factor (HGF) binds to MET and activates its tyrosine kinase activity. MET is overexpressed and activated in a variety of human cancers including pancreatic, colon, gastric, cervical and ovarian cancers and has been shown to be involved in tumor cell migration and invasion (2). MET(T992I) is one of the native mutant forms of MET