Description
Recombinant 2019-nCoV Spike protein S1 subunit, RBD (L452R)(319-541) was expressed in CHO cells using a C-terminal his tag. The gene accession number is MN908947.
The receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein that recognizes the host ACE2 receptor is a major determinant of viral entry and neutralization, and is the most divergent region (1). A new SARS-CoV-2 variant identified in California termed as 20C/S:452R or CAL.20C, possesses L452R as one of the three mutations in the spike protein and this mutation could affect the binding of certain therapeutic monoclonal antibodies. As new variants displace the first-wave virus, it is pivotal to evaluate their transmissibility, virulence and their possible tendency to escape antibody neutralization (3)