Description
Recombinant full-length rat CAMKK1 (F230G) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is L42810. The mutant has induced a structural modification in its ATP binding site and showed a less kinase activity.
CAMKK1 or CAMKKalpha is a Ca(2+)/calmodulin-dependent protein kinase that activates CAM-kinases I and IV via phosphorylation of their Thr(177) and Thr(196) residues, respectively. Recent studies have shown that the activity of CAMKK1 is decreased upon phosphorylation by cAMP-dependent protein kinase (PKA)(1) The CAMKKalpha has been identified in intact cells as AMPKKs, predicting a significant role for this kinase in regulating AMPK activity in vivo. It has been shown that 2-deoxyglucose-and ionomycin-stimulated AMPK activity is substantially reduced in HeLa cells transfected with small interfering RNAs specific for CAMKKalpha (2)