Description
Short Description: Recombinant JMJD2C/KDM4C (accession number NP_055876.2) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK tag with an observed molecular weight of 124.1 kDa. KDM4C (lysine (K)-specific demethylase 4C), also known as JMJD2C (Jumonji Domain Containing 2C) is a nuclear protein that functions as a trimethylation-specific histone demethylase that preferentially demethylates trimethylated lysine 9 (K9me3) and lysine 36 (K36me3) residues of histone H3, converting these trimethylated histone residues to dimethylated form. KDM4C has no activity for mono- and dimethylated H3K9 and H3K36. Chromosomal aberrations and overexpression of KDM4C are associated with esophageal squamous cell carcinoma. KDM4C regulates colonosphere formation in colorectal cancers by mediating the crosstalk between Wnt and Notch signaling pathways. Recombinant JMJD2C/KDM4C can be used for enzyme kinetics and inhibitor screening studies