Description
Short Description: Recombinant Mononucleosomes H3.2-biotin consist of a 167 bp of 601 DNA with 5' biotin tag and two molecules each of histones H2A that includes amino acids 1-130 (end)(accession number NM_003512), H2B that includes amino acids 1-126 (end)(accession number NM_003518), H3.2 that includes amino acids 1-136 (end)(accession number NM_021059.3), and H4 that includes amino acids 1-103 (end)(accession number NM_003548). All of these histones were expressed in E Coli cells. The molecular weight of the histone octamer is 108 kDa. Recombinant Mononucleosomes H3.2-biotin are suitable for use as substrates for histone modification enzymes, or to generate chromatin in vitro