Description
Resistin, also known as adipose tissue-specific secretory factor (ADSF) or C/EBP-epsilon-regulated myeloid specific secreted cysteine-rich protein (XCP1) is a cysteine-rich peptide hormone derived from adipose tissue it participates in the inflammatory response. It plays some role in inflammation, glucose metabolism, and angiogenesis. In primates, pigs, and dogs, resistin is secreted by immune and epithelial cells, while, in rodents, it is secreted by adipose tissue. Although the function of resistin is unclear, it would seem to block insulin-stimulated uptake of glucose by adipocytes and promote glucose release by hepatocytes. As such, it has been proposed to participate in diet?induced insulin-sensitivity. It has been also shown to cause high levels of low-density lipoprotein (LDL), increasing the risk of heart disease. It has been shown to increase transcriptional events, leading to an increased expression of several pro-inflammatory cytokines including IL-1, IL-6, IL-12 and TNF-alpha in an NF-kappaB-mediated fashion. Also, resistin could act as a key node in inflammatory diseases with or without associated insulin resistance. Recombinant human Resistin, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques