BbsI-HF R3539M from New England Biolabs

Product Specs
ItemBbsI-HF
CompanyNew England Biolabs
Price $156.00 Inquire
Catalog NumberR3539M
Temperature-20 C
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Size1000 units

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A harmless-to-harmful switchable and spatiotemporally activated nano-CRISPR hierarchically amplifies ferroptosis in melanomaCell Reports MedicineApril 21, 2026Chunqing Ou, Xianzhou Huang, Dongxue Huang, Rui Luo, Ruhan Chen, Xinchao Li, Xinyue Wu, Qinjie Wu, Changyang Gongacid,Macklin,Cat# M813436 adipic dihydrazide,Macklin,Cat# A800779 DMSO,Macklin,Cat# D806645 BbS I-HF,NEB,Cat# R3539M T4 PNK,NEB,Cat# M0201V T4 DNA ligase,NEB,Cat# M0202V T7E I,NEB,Cat# M0302L glutaraldehyde,Macklin,Cat#

(2) Base editing strategies for in vivo correction of two highly recurrent phenylketonuria variantsMolecular Therapy Nucleic AcidsDecember 9, 2025Aidan Quigley, Ishaan Jindal, Thomas Campion, Delaney Rutherford, Yongseok Han, Walsh Quigley, Ping Qu, Kiran Musunuru, Rebecca Ahrens-Nicklas, Xinying Hong, et al.were annealed and then cloned into pGuide (Addgene #64711) which had been digested with BbsI-HF (NEB #R3539M). For plasmid transfections, HuH-7 cells were seeded on 24-well plates (Corning) at 8 × 104 cells per

(3) ATM deficiency drives phenotypic diversity and Purkinje cell degeneration in a macaque model of ataxia-telangiectasiaCell Reports MedicineSeptember 16, 2025Kaiyu Xu, Ying Zhang, Yongxuan Chen, Xiaojia Zhu, Yu Li, Longbao Lv, Xiechao He, Zhengfei Hu, Yifan Li, Maosen Ye, et al.25200072 GlutaMAX,Gibco,Cat# 35050061 37% formaldehyde,Sigma-Aldrich,Cat# F1635 BbsI-HF,NEB,Cat# R3539M T4 DNA ligase,NEB,Cat# M0202 T4 DNA polymerase,NEB,Cat# M0203 Lipofectamine 3000,Thermo Fisher,Cat#

(4) APE1 condensation in nucleoli of non-cancer cells depends on rRNA transcription and forming G-quadruplex RNA structuresNucleic acids researchFebruary 27, 2025Dall''Agnese G, Hannett NM, Overholt KJ, Platt JM, Henninger JE, Marcos-Vidal A, Othman Z, Salgado G, Antoniali G, Tell G.instructions. gRNA–Cas9 plasmids were generated upon cleavage with the BbsI restriction enzyme (NEB, R3539M) of the px330 plasmid following NEB instructions; the cleaved plasmid was run on gel and purified. Complementary

(5) An efficient cloning method to expand vector and restriction site compatibility of Golden Gate AssemblyCell Reports MethodsAugust 22, 2023Masato Sorida, Roberto Bonasio,Thermo Scientific,SM1331 BsaI-HFv2,NEB,R3733S BbsI-HF,NEB,R3539S AgeI-HF,NEB,R3552S BamHI-HF,NEB,R3136S EcoRI-HF,NEB,R3101L HindIII-HF,NEB,R3104S NcoI-HF,NEB,R3193S NotI-HF,NEB,R3189S SalI-HF,NEB,R3138S SpeI-HF,NEB,R3133S XbaI,NEB,R0145S XhoI,NEB,R0146S XmaI,NEB,R0180S KpnI-HF,NEB,R3142S NdeI,NEB,R0111S Smal,NEB,R0141S BbsI-HF,NEB,R3539M T4 DNA Ligase,NEB,M0202L Hi-T4 DNA Ligase,NEB,M2622S 100 mM ATP,NEB,P0756S NEBuilder HiFi DNA Assembly Master Mix

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BbsI-HF

BbsI-HF from New England Biolabs

New England Biolabs

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BbsI-HF from New England Biolabs

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