Description
A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutation N501Y) (Arg319-Phe541) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The purified protein was biotinylated in vitro. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.1.7) which emerged in the UK