Human PD-L1 Protein (His & AVI Tag), Biotinylated 10084-H49H-B from Sino Biological, Inc.

Product Specs
ItemHuman PD-L1 Protein (His & AVI Tag), Biotinylated
CompanySino Biological, Inc.
Price $298.00Supplier Page View Company Product Page
Catalog Number10084-H49H-B
TypeRecombinant protein
Molecule NameCD274
Conjugate/TagC-His-AVI
Size20 µg
SpeciesHuman
NCBI Full Gene NameCD274 molecule
NCBI Gene AliasesB7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1
FormatLyophilized from sterile PBS, pH 7.4
Source/Expression SystemHEK293 Cells
Molecular WeightThe recombinant human CD274 consists of 246 amino acids and predicts a molecular mass of 28.48 kDa. It migrates as an approximately 38.01 kDa band in SDS-PAGE under reducing conditions
Purity≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC
Activity1.Immobilized Recombinant Human PD-L1 Protein(His & AVI Tag), Biotinylated(Cat: 10084-H49H-B) at 2 µg/mL(100 µl/well) on streptavidin precoated(2 µg/mL, 100 µL/well) can bind Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag)(Cat: 10377-H02H), the EC50 is 3-10 ng/mL(QC tested). 2.Immobilized Anti-PDL1 Antibody(Atezolizumab), Human IgG1 at 2 µg/mL(100 µL/well) can bind Recombinant Human PD-L1 Protein(His & AVI Tag), Biotinylated(Cat: 10084-H49H-B), the EC50 is 2-6 ng/mL(Rountinely tested). 3.Immobilized Recombinant Human PD-L1 Protein(His & AVI Tag), Biotinylated(Cat: 10084-H49H-B) at 1 µg/mL(100 µL/well) on streptavidin precoated(5 µg/mL, 100 µL/well) plate can bind Anti-PDL1(Research grade Atezolizumab biosimilar), the EC50 is 1-4 ng/mL(Routinely tested). 4.Immobilized Recombinant Human PD-L1 Protein(His & AVI Tag), Biotinylated(Cat: 10084-H49H-B) at 1 µg/mL(100 µL/well) on streptavidin precoated(5 µg/mL, 100 µL/well) plate can bind Recombinant Human PD1 / PDCD1 / CD279 Protein(ECD, Fc Tag)(Cat: 10377-H05H), the EC50 is 3.3 ng/mL(Routinely tested)