Immunohistology staining on PD-L2 overexpressed cytosection (TS424141) with mouse anti PD-L2 antibody (UM800115) at 1:200 dilution, 1hr room temperature. The staining was achieved with HIER Tris/EDTA buffer, pH9.0, 100oC for 20 min, Polink2 detection system (D22-18) with DAB chromogen. HEK293T cells were transfected with cDNA clone RC202141, 4um sections, 40x magnification

Immunohistology staining of untransfected HEK293T with mouse anti PD-L2 antibody (UM800115) at 1:200 dilution, 1 hour at room temperature. The staining was conducted with HIER Tris/EDTA buffer, pH9.0, 100oC for 20 min, Polink2 detection system with DAB chromogen (D22-18). HEK293T cells were 4um sections, 40x magnification.

Immunohistology staining on PD-L2 overexpressed cytosection (TS424141) with mouse anti DDK clone 11C3 C/N TA180144 at 1:1000 dilution 4C O/N. Antibody staining was achieved with HIER Tris/EDTA pH9 for 20minutes, Polink2 detection system with DAB chromogen (D22-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC202141, 4um sections, 40x magnification

Immunohistology staining on HEK293T untransfected control section screened with mouse anti-DDK clone 11C3 (TA180144) at 1:1000 dilution 4C O/N. Antibody staining was achieved with HIER Tris/EDTA pH9 for 20minutes, Polink2 detection system with DAB chromogen (D22-18). No brown chromogen showing negative stain.