Trypsin Platinum, Mass Spectrometry Grade VA9000 from Promega

Product Specs
ItemTrypsin Platinum, Mass Spectrometry Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberVA9000
Size100µg
ApplicationsMass Spectrometry

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Identification and characterization of a novel aldehyde metabolite of WIN18,446 and associated WIN18,446-ALDH1A2 protein adducts using mass spectrometryDrug Metab RevMay 13, 2026Alex Zelter, Guo Zhong, Michal Maes, Michael R. Hoopmann, Michael Riffle, Daniel Jaschob, Eddie A. Carlson Ii, Wendel L. Nelson, Deepak Dalvie, Robert L. Moritz, et al.concentration) prior to digestion at a 1:15 (enzyme:substrate ratio) using trypsin (Promega Platinum, cat no. VA9000) for 12 hours at 37°C in an Eppendorf Thermomixer with shaking (1000 rpm). After digestion, samples

(2) Sequestration of growth cone surface proteins by cytoplasmic Lrrtm2 induces de novo amygdala innervation by cerebral cortex associative neuronsbioRxivApril 12, 2026Dustin E. Tillman, Omer Durak, Priya Veeraraghavan, John E. Froberg, Garrett Wheeler, Bogdan Budnik, Jeffrey D. Macklis(Millipore #UFC8010), and frozen at −80 °C. Samples were then thawed, digested by Trypsin Platinum (Promega #VA9000), and run on an Orbitrap Astral mass spectrometer (Thermo #BRE725600). GC samples, which contain sucrose,

(3) A Dimeric Rocaglate Promotes Multivalent eIF4A-RNA AssemblybioRxivMarch 13, 2026Jie Liu, Megan K. Moore, Kevin Lou, Douglas R. Wassarman, Abolfazl Arab, Samuel Ojeda, Barbara Karakyriakou, Ann-Sophie Koglin, Christopher J. Ott, Luke A. Gilbert, et al.(Sigma, I6125) for 30 min in the dark. On-bead digestion was performed using 2 μg trypsin (Promega, VA9000) in 100 μL 100 mM TEAB (Sigma, T7408) at 37 °C overnight. The supernatant was collected, and beads were

(4) Nuclear import of malaria RNA rewires splicing in host immune cellsCell ReportsFebruary 24, 2026Paula Abou Karam, Edo Kiper, Tamar Ziv, Shaked Yadid, Ewa Kozela, Nir Zharoni, Reinat Nevo, Daniel Alfandari, Helina Otesh, Abel Cruz Camacho, et al.20 μg aliquots were digested in 2 M urea, 25 mM ammonium bicarbonate with modified trypsin (Promega, VA9000), overnight at 37°C in a 1:50 enzyme-to-substrate ratio. The tryptic peptides were desalted using Top

(5) Candidate biomarkers to identify mesothelioma patients at risk of developing venous thromboembolism post-surgeryScientific ReportsFebruary 16, 2026Adnan Shami-shah, Shira Roth, Shad R. Morton, Bogdan Budnik, William G. Richards, Raphael Bueno, Rushdy Ahmad, David R. Waltand proteins were digested overnight at 37 °C with mass-spectrometry grade Platinum Trypsin (Promega VA9000) at a 1:50 enzyme-to-substrate ratio. Tandem mass Tag (TMT) labeling Peptides were labeled with TMTpro

(6) Legionella employs the multimodal ubiquitination of Sec22b to modulate SNARE pairingiScienceJanuary 16, 2026Tomoe Kitao, Rina Iida, Hideki Kondo, Yoshiki Miura, Hikari Taka, Susan Kiiru, Toru Okamoto, Kohei Arasaki, Hideaki Higashi, Tomoko Kubori, et al.C0267 Triethylammonium bicarbonate (TEAB),Merk,Cat# 18597 "Trypsin Platinum, Mass Spectrometry Grade",Promega,Cat# VA9000 Acetonitrile,Wako/Fujifilm,Cat# 014-00386 ,, Critical commercial assays,Critical commercial assays,Critical

(7) Deletion of neuronal Idol ameliorates Alzheimer's disease-related pathologies via APOE receptorsAlzheimers DementDecember 12, 2025Hande Karahan, Kelly Hartigan, Md Mamun Al-Amin, Sutha K. John, Brianne McCord, H. R. Sagara Wijeratne, Dominic J. Acri, Daniel C. Smith, Luke C. Dabin, Hannah M. Rondon Cordero, et al.37°C, treated with PNGase F (New England BioLabs, P0705L), and digested with Trypsin Platinum (Promega, VA9000) and quenched with trifluoroacetic acid. 29 , 30 The peptides were desalted with Waters Sep‐Pak Vac

(8) Integrative multi-omics analysis in vivo identifies influenza virus host factorsiScienceNovember 21, 2025Shriver LP, Polacco BJ, Eckhardt M, Chang MW, Martínez-Romero C, Adkins-Travis K, Cahalane C, Haas K, Stein DA, Fourati S, et al.C4706-2G Iodoacetamide,Millipore Sigma,Cat# I1149-5G DL-Dithiothreitol,Millipore Sigma,Cat# D0632-1G Trypsin,Promega,Cat# VA9000 Sodium pyruvate,Fisher Scientific,Cat# MT 10-017-CV Fetal bovine serum,Life Technologies,Cat# A3160502 1X

(9) Combinatorial protein engineering identifies potent CRISPR activators with reduced toxicityNature CommunicationsNovember 20, 2025Marla Giddins, Alexander F. Kratz, Mark B. De Los Santos, Antoine Forget, Richa Tiwari, Gwendolyn Jang, Tomasz Blazejewski, Chuyan Qin, Yiming Huang, Yeh-Hsing Lao, et al.addition of 3 mM iodoacetamide and quenched for 10 minutes with 3 mM DTT. Trypsin (1.5 μg; Promega, Cat. #VA9000) was added and incubated at 37 °C, overnight with shaking at 1,000 rpm. Peptides were acidified with

(10) Development and Validation of a Novel LC-MS/MS-Based Proteomics Method for Quantitation of Retinol Binding Protein 4 (RBP4) and Transthyretin (TTR)Journal of Proteome ResearchOctober 3, 2025Aprajita S. Yadav, Katya B. Rubinow, Alex Zelter, Aurora K. Authement, Bryan R. Kestenbaum, John K. Amory, Nina IsoherranenRinger’s solution were purchased from Thermo Fisher Scientific (Waltham, MA). Trypsin Platinum (PN VA9000) was purchased from Promega (Madison, WI). RapiGest SF was purchased from Waters (Milford, MA). Optima

(11) Protein Turnover Dynamics Analysis With Subcellular Spatial ResolutionBIO-PROTOCOLAugust 5, 2025Lorena Alamillo, Alexander Black, Maggie P.Y. Lam, Edward Lau148415000) 18. TCEP (Thermo, catalog number: PI77720) 19. Sequencing-grade trypsin (Promega, catalog number: VA9000) 20. TMT 10-plex (Thermo, catalog number: 90309) 21. LC–MS anhydrous acetonitrile (Thermo, catalog number:

(12) Protein Turnover Dynamics Analysis With Subcellular Spatial ResolutionBio ProtocAugust 5, 2025Lorena Alamillo, Alexander Black, Maggie P.Y. Lam, Edward Lau148415000) 18. TCEP (Thermo, catalog number: PI77720) 19. Sequencing-grade trypsin (Promega, catalog number: VA9000) 20. TMT 10-plex (Thermo, catalog number: 90309) 21. LC–MS anhydrous acetonitrile (Thermo, catalog number:

(13) Sex and Age but Not Body Mass Index (BMI) Predict Serum Retinol Binding Protein 4 (RBP4) and Transthyretin (TTR) ConcentrationsFASEB JJuly 31, 2025Aprajita S. Yadav, Lindsay C. Czuba, Jerry Zhu, Jeffrey LaFrance, Aurora K. Authement, Lindsey Williams, Judy Y. Chen, Estell Williams, Zoe Parr, Saurabh Khandelwal, et al.Ringer's solution were purchased from Thermo Fisher Scientific (Waltham, MA). Trypsin Platinum (PN VA9000) was purchased from Promega (Madison, WI). Optima LC–MS grade acetonitrile, water, acetic acid, and

(14) Sex and Age but Not Body Mass Index (BMI) Predict Serum Retinol Binding Protein 4 (RBP4) and Transthyretin (TTR) ConcentrationsFASEB JJuly 28, 2025Aprajita S. Yadav, Lindsay C. Czuba, Jerry Zhu, Jeffrey LaFrance, Aurora K. Authement, Lindsey Williams, Judy Y. Chen, Estell Williams, Zoe Parr, Saurabh Khandelwal, et al.Ringer's solution were purchased from Thermo Fisher Scientific (Waltham, MA). Trypsin Platinum (PN VA9000) was purchased from Promega (Madison, WI). Optima LC–MS grade acetonitrile, water, acetic acid, and

(15) Increasing mass spectrometry throughput using time-encoded sample multiplexingbioRxivMay 31, 2025Jason Derks, Kevin McDonnell, Nathan Wamsley, Peyton Stewart, Maddy Yeh, Harrison Specht, Nikolai Slavovconcentration of 100 mM, Benzonase nuclease (E1014, Millipore) was added to 0.1 U/μL, and Trypsin Platinum (VA9000, Promega) was added to a concentration of 15 ng/μL. Single-cell sample preparation using nPOP Single

(16) Proteomic analysis of B cells in peripheral lymphatic system reveals the dynamics during the systemic lupus erythematosus progressionBiophysics ReportsApril 30, 2025Liming Sun, Yuanyuan Yin, Yuqing Cao, Chunlei Chen, Yutong Guo, Zeming Cai, Jiarui Wu, Qingrun Lihydrochloride (TCEP), 20 mmol/L 2-Chloroacetamide (CAA), pH 8), and mixed with 3 μL trypsin (Promega, VA9000). The samples were then incubated at 56 °C in a water bath for 1 h for digestion. Following digestion,

(17) Dietary effects on the retina of hamstersFASEB JMarch 31, 2025Nicole El-Darzi, Natalia Mast, Yong Li, Irina A. Pikulevadithiothreitol, alkylated with 50 mM iodoacetamide, and digested with trypsin (Promega, Madison, WI, USA, #VA9000). The trypsinolysis was quenched with 0.1% trifluoroacetic acid, and gel slices were removed by centrifugation.

(18) A novel in-vitro expression assay by LC/MS/MS enables multi-antigen mRNA vaccine characterizationSci RepMarch 25, 2025Hanliu Leah Wang, Kimia Kajbaf, Brian C. Gau, Andrew W. Dawdy, Rachel Edwards, Bradley Bare, Gianna Raymundo, Jose Iturrizaga, Chase Ernsky, Michael Walker, et al.(Cat#797,004) from BioLegend. Additionally, Promega Trypsin Platinum, Mass Spectrometry Grade (Cat#VA9000) and FujiFilm Wako Pure Chemical Corporation Lysyl Endopeptidase, Mass Spectrometry Grade (Lys-C) 10AU/Vial

(19) Extracellular vesicles from the lung pro-thrombotic niche drive cancer-associated thrombosis and metastasis via integrin beta 2CellMarch 20, 2025Serena Lucotti, Yusuke Ogitani, Candia M. Kenific, Jacob Geri, Young Hun Kim, Jinghua Gu, Uthra Balaji, Linda Bojmar, Lee Shaashua, Yi Song, et al.#233155 Iodoacetamide,Sigma-Aldrich,Cat #I1149 Lysyl Endopeptidase®,Fujifilm Wako,Cat #125–05061 Trypsin Platinum,Promega,Cat #VA9000 Trifluoroacetic acid,Thermo Fisher,Cat #85183 Acetonitrile,Fisher Scientific,Cat #A955–4 Formic Acid,Fisher

(20) Dietary effects on the retina of hamstersFASEB JMarch 18, 2025Nicole El‐Darzi, Natalia Mast, Yong Li, Irina A. Pikulevadithiothreitol, alkylated with 50 mM iodoacetamide, and digested with trypsin (Promega, Madison, WI, USA, #VA9000). The trypsinolysis was quenched with 0.1% trifluoroacetic acid, and gel slices were removed by centrifugation.

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Trypsin Platinum, Mass Spectrometry Grade

Trypsin Platinum, Mass Spectrometry Grade from Promega

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Promega

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Trypsin Platinum, Mass Spectrometry Grade from Promega

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