SARS-CoV-2 Spike RBD (L452R,T478K) Protein (His Tag) 40592-V08H90 from Sino Biological, Inc.

Product Specs
ItemSARS-CoV-2 Spike RBD (L452R,T478K) Protein (His Tag)
CompanySino Biological, Inc.
Price $448.00Supplier Page View Company Product Page
Catalog Number40592-V08H90
TypeRecombinant protein
Molecule NameSpike
SpeciesSARS-CoV-2
FormatLyophilized from sterile PBS, pH 7.4
Conjugate/TagC-His
Source/Expression SystemHEK293 Cells
Molecular WeightThe recombinant SARS-CoV-2 Spike RBD consists of 234 amino acids and predicts a molecular mass of 26.6 kDa. As a result of glycosylation, it migrates as an approximately 36.9 kDa band in SDS-PAGE under reducing conditions
Purity> 95 % as determined by SDS-PAGE
Size100 µg
ActivityImmobilized ACE2 Protein, Human, Recombinant (mFc Tag)(Cat: 10108-H05H) at 2 µg/mL (100 µL/well) can bind SARS-CoV-2 Spike RBD (L452R,T478K) Protein (His Tag)(Cat:40592-V08H90), the EC50 of SARS-CoV-2 Spike RBD (L452R,T478K) Protein (His Tag)(Cat: 40592-V08H90) is 4-24 ng/mL

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Sino Biological, Inc.

37 Industrial Blvd

Suite B

Paoli, PA 19301

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: sinobiological.com

(1) Comprehensive N-glycosylation profiling of recombinant spike S1 protein from the wild-type SARS-CoV-2 and its variantsFront ImmunolJuly 16, 2025Yao Peng, Tian-Tian Tong, Qiu-Yu Deng, Lee-Fong Yau, Jia-Qi Qiu, Qing Zhao, Jia-Qi Wu, Zhi-Qiang Xin, Man-Ci Guan, Yue Li, et al.RBD-Gamma, Cat. No. 40592-V08H86), Delta S1 protein (S1-Delta, Cat. No. 40591- V08H23; RBD-Delta, Cat. No. 40592-V08H90), Lambda S1 protein (S1- Lambda, Cat. No. 40591- V08H31; RBD- Lambda, Cat. No. 40592-V08H113) forms

(2) Identification of an Unnatural Sulfated Monosaccharide as a High-Affinity Ligand for Pan-Variant Targeting of SARS-CoV-2 Spike GlycoproteinACS chemical biologyJune 20, 2025Thompson A, Sankaranarayanan NV, Chittum JE, Mahida V, Vishweshwara SS, Raigawali R, Anand S, Kikkeri R, Desai UR.

(3) A humanised ACE2, TMPRSS2, and FCGRT mouse model reveals the protective efficacy of anti-receptor binding domain antibodies elicited by SARS-CoV-2 hybrid immunityEBioMedicineMarch 1, 2025Ana-Sosa-Batiz F, Verma SK, Shafee N, Miller R, Conner C, Hastie KM, Timis J, Maule E, Nguyen MN, Tran L, et al.Delta or Omicron BA.5 Spike or RBD proteins (Sino Biological, catalogue # 40589-V08H10, 40589-V08H33, 40592-V08H90, and 40592-V08H131, respectively). The wells were then blocked for 1 h at room temperature with 5% nonfat

(4) Evolution of SARS-CoV-2 spike trimers towards optimized heparan sulfate cross-linking and inter-chain mobilityScientific ReportsDecember 31, 2024Jurij Froese, Marco Mandalari, Monica Civera, Stefano Elli, Isabel Pagani, Elisa Vicenzi, Itzel Garcia-Monge, Daniele Di Iorio, Saskia Frank, Antonella Bisio, et al.variant (Wuhan or 2019 nCoV, Sinobiological 40592-V08H), of the B.1.167.2 variant (Delta, Sinobiological 40592-V08H90), and of the B.1.1.529 variant (Omicron, Sinobiological 40592-V08H121). S1 + S2 homo-trimers of the

(5) An ancestral SARS-CoV-2 vaccine induces anti-Omicron variants antibodies by hypermutationNature communicationsApril 20, 2024Park S, Choi J, Lee Y, Noh J, Kim N, Lee J, Cho G, Kim S, Yoo DK, Kang CK, et al.

(6) Structure-Based Optimization of One Neutralizing Antibody against SARS-CoV-2 Variants Bearing the L452R MutationVirusesApril 5, 2024Chen Y, Zha J, Xu S, Shao J, Liu X, Li D, Zhang X.

(7) SARS-CoV-2 spike protein receptor binding domain promotes IL-6 and IL-8 release via ATP/P2Y(2) and ERK1/2 signaling pathways in human bronchial epitheliaMolecular immunologyMarch 1, 2024Zhang RG, Liu XJ, Guo YL, Chen CL.

(8) The Immunogenicity of CpG, MF59-like, and Alum Adjuvant Delta Strain Inactivated SARS-CoV-2 Vaccines in MiceVaccines (Basel)January 7, 2024Kangwei Xu, Jing Li, Xu Lu, Xiaoqin Ge, Kaiqin Wang, Jiahao Wang, Zhizhong Qiao, Yaru Quan, Changgui Lior RBD proteins (SinoBiological, Beijing, China, N Cat: 40588-V07E29, S Cat: 40589-V08B16, RBD Cat: 40592-V08H90) were diluted to 1 μg/mL using PBS. Ninety-six-well ELISA plates were coated with antigens at 100 μL/well

(9) Monovalent Omicron COVID-19 vaccine triggers superior neutralizing antibody responses against Omicron subvariants than Delta and Omicron bivalent vaccineHuman Vaccines & ImmunotherapeuticsOctober 17, 2023Wanting Li, Tiantian Zhao, Bai Tao, Liwei Zhao, Hang Xiao, Xinyu Ding, Chuang Li, Lin Chen, Hao Cheng, Yang Lou, et al.SARS-CoV-2 RBD protein (Cat #: 40592-V08B, Sino Biological) and SARS-CoV-2 B.1.617.2 RBD protein (Cat #: 40592-V08H90, Sino Biological) were purchased for immunization. SARS-CoV-2 Delta spike protein (Cat #:40592-V08H91,

(10) Assessment of safety and intranasal neutralizing antibodies of HPMC-based human anti-SARS-CoV-2 IgG1 nasal spray in healthy volunteersScientific ReportsSeptember 20, 2023Thanarath Imsuwansri, Thitinan Jongthitinon, Niramon Pojdoung, Nuntana Meesiripan, Siriwan Sakarin, Chatikorn Boonkrai, Tossapon Wongtangprasert, Tanapati Phakham, Thittaya Audomsun, Chadaporn Attakitbancha, et al.dilution were added (100 µl/well) to an ELISA plate coated with 100 ng/well of Delta-variant RBD proteins (40592-V08H90, Sino Biological). Human IgG1 anti-SARS-CoV-2 antibodies were detected with goat anti-human IgG Fcγ-HRP

(11) Spike-protein proteolytic antibodies in COVID-19 convalescent plasma contribute to SARS-CoV-2 neutralizationCell chemical biologyJuly 20, 2023McConnell SA, Sachithanandham J, Mudrak NJ, Zhu X, Farhang PA, Cordero RJB, Wear MP, Shapiro JR, Park HS, Klein SL, et al.,Sino Biologicals,Cat#40592-V08H18 Recombinant RBD (E484K),Sino Biologicals,Cat#40592-V08H84 Recombinant RBD (L452R/T478K),Sino Biologicals,Cat#40592-V08H90 Recombinant RBD (Omicron; G339D/S371L/S373P/S375F/K417N/N440K/G446S/S477N/T478K/E484A/Q493R/G496S/Q498R/N501Y/Y505H),Sino Biologicals

(12) Discovery of Peptidic Ligands against the SARS-CoV-2 Spike Protein and Their Use in the Development of a Highly Sensitive Personal Use Colorimetric COVID-19 BiosensorACS sensorsJune 23, 2023Yu X, Pan B, Zhao C, Shorty D, Solano LN, Sun G, Liu R, Lam KS.C-His-tag) and Delta-variant SARS-CoV-2 spike protein receptor-binding domain (L452R, T478K) (Catalog No. 40592-V08H90) were purchased from Sino Biological (Wayne, PA). The SARS-CoV-2 spike protein active trimer (Catalog

(13) Enhancement of SARS-CoV-2 receptor-binding domain activity by two microbial defensinsFrontiers in MicrobiologyJune 19, 2023Bin Gao, Shunyi ZhuKMPH1835). Recombinant δRBD and oSpike were purchased from Sino Biological (Beijing, China) (Cat No. 40592-V08H90). All these purchased protein products were prepared from mammalian cells. The experiments were performed

(14) Discovery and characterization of potent pan-variant SARS-CoV-2 neutralizing antibodies from individuals with Omicron breakthrough infectionNature communicationsJune 15, 2023Guo Y, Zhang G, Yang Q, Xie X, Lu Y, Cheng X, Wang H, Liang J, Tang J, Gao Y, et al.μg/mL His-tagged WT RBD (Sino Biological Inc, #40592-V08B), 0.04 μg/mL Delta RBD (Sino Biological Inc, #40592-V08H90) or 0.01 μg/mL Omicron BA.1.1 RBD (Sino Biological Inc, #40592-V08H121) was added to each well. In addition,

(15) Hybrid immunity expands the functional humoral footprint of both mRNA and vector-based SARS-CoV-2 vaccinesCell Reports MedicineMay 16, 2023Paulina Kaplonek, Yixiang Deng, Jessica Shih-Lu Lee, Heather J. Zar, Dace Zavadska, Marina Johnson, Douglas A. Lauffenburger, David Goldblatt, Galit Alter(B.1.617.2) Spike,Sino Biological,CAT#:40589-V08H10 SARS-CoV-2 Delta (B.1.617.2) RBD,Sino Biological,CAT#:40592-V08H90 SARS-CoV-2 Omicron (B.1.1529) Spike,Sino Biological,CAT#:40589-V49H3-B SARS-CoV-2 Omicron (B.1.1529)

(16) Protocol to determine antibody affinity and concentration in complex solutions using microfluidic antibody affinity profilingSTAR protocolsMarch 17, 2023Emmenegger M, Worth R, Fiedler S, Devenish SRA, Knowles TPJ, Aguzzi A.SARS-CoV-2 RBD, HEK293",Sino Biological,40592-V08H "Delta SARS-CoV-2 RBD, HEK293",Sino Biological,40592-V08H90 "Omicron SARS-CoV-2 RBD, HEK293",Sino Biological,40592-V08H121 Alexa Fluor™ 647 NHS ester,Thermo Scientific,A20006 Software,Software,Software Fluidity

(17) Biparatopic antibody BA7208/7125 effectively neutralizes SARS-CoV-2 variants including Omicron BA.1-BA.5Cell discoveryJanuary 7, 2023Wang Y, Yan A, Song D, Dong C, Rao M, Gao Y, Qi R, Ma X, Wang Q, Xu H, et al.using in-tandem format binning assay. Biotinylated RBD protein of Delta variant (Sino Biological, Cat. 40592-V08H90) was loaded onto SA sensors (Fortebio, Cat. 18-5019). The sensors were then exposed to the first antibody

(18) Bivalent SARS-CoV-2 mRNA vaccines increase breadth of neutralization and protect against the BA.5 Omicron variant in miceNature MedicineJanuary 1, 2023Suzanne M. Scheaffer, Diana Lee, Bradley Whitener, Baoling Ying, Kai Wu, Chieh-Yu Liang, Hardik Jani, Philippa Martin, Nicholas J. Amato, Laura E. Avena, et al.filters (EMD Millipore). SARS-CoV-2 B.1.617.2 RBD protein was purchased from Sino Biological (Cat. # 40592-V08H90). ELISA. Assays were performed in 96-well microtiter plates (ThermoFisher Scientific) coated with

(19) Oxalic acid blocked the binding of spike protein from SARS-CoV-2 Delta (B.1.617.2) and Omicron (B.1.1.529) variants to human angiotensin-converting enzymes 2PloS oneJanuary 1, 2023Wang M, Yan H, Chen L, Wang Y, Li L, Zhang H, Miao L.using a BIAcore T200 instrument. RBDs of Delta B.1.617.2 (Sino Biological, Beijing, China, Cat. No.40592-V08H90, 50 μg/ml) and Omicron B.1.1.529 (GenScrip, Nanjing, China, Cat. No. Z03728, 50 μg/ml) and ACE2 (Sino Biological Inc

(20) Development and Characterization of Phage-Display-Derived Novel Human Monoclonal Antibodies against the Receptor Binding Domain of SARS-CoV-2BiomedicinesDecember 17, 2022Kim JW, Min SW, Lee J, Shin HG, Choi HL, Yang HR, Lee JH, Cho YB, Shim H, Lee S.Biological), Alpha variant-RBD-His (Cat#40592-V08H82, Sino Biological), and Delta variant-RBD-His (Cat#40592-V08H90, Sino Biological) were pre-incubated in the absence or presence of increasing concentrations of mAbs

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SARS-CoV-2 Spike RBD (L452R,T478K) Protein (His Tag) from Sino Biological, Inc.

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Sino Biological, Inc.

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SARS-CoV-2 Spike RBD (L452R,T478K) Protein (His Tag) from Sino Biological, Inc.

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