Human TIGIT Protein (ECD, mFc Tag) 10917-H38H from Sino Biological, Inc.

Product Specs
ItemHuman TIGIT Protein (ECD, mFc Tag)
CompanySino Biological, Inc.
Price $1,158.00Supplier Page View Company Product Page
Catalog Number10917-H38H
TypeRecombinant protein
Molecule NameTIGIT
SpeciesHuman
NCBI Full Gene NameT cell immunoreceptor with Ig and ITIM domains
NCBI Gene AliasesVSIG9, VSTM3, WUCAM
FormatLyophilized from sterile PBS, pH 7.4
Source/Expression SystemHEK293 Cells
Molecular WeightThe recombinant human TIGIT consists of 353 amino acids and predicts a molecular mass of 39.4 kDa. As a result of glycosylation, it migrates as an approximately 45-54 kDa band in SDS-PAGE under reducing conditions
Conjugate/TagC-mouse IgG2a-Fc
Purity≥ 95 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC
Activity1.Immobilized Human TIGIT(mFc Tag)(Cat:10917-H38H) at 2 µg/mL (100 µL/well) can bind Human CD155/PVR (hFc & AVI Tag), Biotinylated(Cat:10109-H41H-B), the EC50 of Human CD155/PVR (hFc & AVI Tag), Biotinylated(Cat:10109-H41H-B) is 15-120 ng/mL(QC tested). 2.Loaded Recombinant Human TIGIT Protein, mFc Tag (Cat. No. 10917-H38H) on proA Biosensor, can bind Recombinant Human CD155/PVR Protein, His Tag (Cat. No. 10109-H08H) with an affinity constant of 1.380 µM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 3.Serial dilutions of anti-TIGIT (Research Grade Tiragolumab biosimilar) were added into Recombinant Human TIGIT Protein (ECD, mFc Tag) (Cat: 10917-H38H): Recombinant Human Nectin-2 Protein (Fc & AVI Tag), Biotinylated (Cat: 10005-H41H-B) binding reactions. The half maximal inhibitory concentration (IC50) is 228.8 ng/mL (Routinely tested)
Size500 µg