Human IL-6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-6 is greater than 90% as determined by SEC-HPLC.
Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-6 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 32.6ng/ml determined by ELISA.
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.1 - 0.5 ng/mL (QC Test).
Supplier Page from Kactusbio Inc. for Human IL-6 Protein (IL6-HE001)