Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-1, His-Avi Tag with an affinity constant of 22.83 nM as determined in SPR assay (Biacore T200).
Immobilized Human Nectin-3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Nectin-1, His Tag with the EC50 of 0.52ug/ml determined by ELISA (QC Test).
Biotinylated Human Nectin-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Nectin-1 is greater than 90% as determined by SEC-HPLC.
Supplier Page from Kactusbio Inc. for Biotinylated Human Nectin-1/PVRL1/CD111 Protein (NEC-HM401B)