The purity of Human IL-7 is greater than 95% as determined by SEC-HPLC.
Human IL-7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human IL-7R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-7, No Tag with an affinity constant of 22.66 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-7 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 55.1ng/ml determined by ELISA.
Measured in a cell proliferation assay using murine 2E8 cells. The ED50 for this effect is 0.1 - 0.5 ng/mL (QC tested).
Supplier Page from Kactusbio Inc. for Human IL-7 Protein (IL7-HE001)