Description
Pseudotyped Luciferase VSV is designed as a control for CD's Pseudotyped Luciferase rSARS-CoV-2 Spike to test for non-specific factors that affect virus infectivity. The Pseudovirus display the VSV envelope glycoprotein (VSV-G) pseudotyped on replication-incompetent virus particles that contain a heterologous lentiviral (HIV) core. Pseudovirus is capable of a single round of infection and carry a genome that expresses luciferase optical reporter gene upon infection. VSV Pseudovirus is produced in HEK-293T cells using three separate plasmids, encoding VSV-G, a lentiviral gag polyprotein, and a reporter gene. VSV Pseudovirus is created using a second-generation lentiviral system with components that are highly unlikely to recombine to produce a fully infectious virus (requiring 3 separate recombination events to do so)