Description
Recombinant full-length 2019-nCoV 3CLpro, part of a large replicase polyprotein 1ab (S3264-Q3569), was expressed in E Coli cells with an N-terminal His tag.
Overview: SARS-CoV-2 3C-like proteinase (3CLpro) is the nonstructural protein number 5, also a cysteine protease, that is released from the virally encoded polyprotein by extensive proteolytic processing. The 3CLpro cleaves the C-terminus of the large replicase polyprotein 1ab (~790 kDa) at no fewer than 11 sites (1). Inhibiting the activity of this enzyme would block viral replication (2). The functional importance of 3CLpro in the viral life cycle makes this protease an attractive target for the development of drugs directed against SARS and other Corona virus infections (3)