PaqCI R0745L from New England Biolabs

Product Specs
ItemPaqCI
CompanyNew England Biolabs
Price $440.00 Inquire
Catalog NumberR0745L
Temperature-20 C
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Size1000 units

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Phage-assisted evolution of allosteric protein switchesNat CommunApril 14, 2026Nicholas T. Southern, Anna von Bachmann, Alisa Hovsepyan, Marielouise Griebl, Benedict Wolf, Nina Lemmen, Ann-Sophie Kroell, Simon Westermann, Jan Mathony, Dominik Niopek(New England Biolabs, cat. no. R0734S), BsaI-HF®v2 (R3733S), BbsI (R0539S), SapI (R0569S), and PaqCI® (R0745S). Plasmids were assembled via Golden Gate cloning. Plasmids were purified using the QIAprep Spin Miniprep

(2) Modular gene tagging in C. elegansResearch SquareFebruary 10, 2026Erik Jorgensen, Kevin Hefel, Kevin Kruse, Soren B. Jorgensen, Kam Hoe Jorgensen, Ryan Stolley, Matthew S. Stolley, Matthew JorgensenpAJH93, a 20 μL mix of 2 μL T4 ligase buffer (NEB® B0202), 2 μL T4 ligase (NEB®, M0202), 2 μL PaqCI (NEB® R0745), 2 μl PaqCI activator (NEB® S0532), 0.1 pmol of each plasmid, 0.3 pmol of annealed oligos, and water

(3) Stepwise DNA unwinding gates TnpB genome-editing activitybioRxivJanuary 28, 2026Zehan Zhou, Iren Saffarian-Deemyad, Honglue Shi, Trevor Weiss, Muhammad Moez ur-Rehman, Kamakshi Vohra, Petr Skopintsev, Peter H. Yoon, Marena I. Trinidad, Conner J. Langeberg, et al.(New England Biolabs, R3539), BsmBI (New England Biolabs, R0739), and PaqCI (AarI; New England Biolabs, R0745) in combination with T4 DNA Ligase (New England Biolabs, M0202). Gibson assemblies were performed with

(4) Modular gene tagging in C. elegansbioRxivJanuary 18, 2026Adam Hefel, Kevin Kruse, Kaden Wall, Soren B. Jorgensen, Kam Hoe Ng, Ryan Stolley, Matthew S. Rich, Erik M. JorgensenpAJH93, a 20 μL mix of 2 μL T4 ligase buffer (NEB® B0202), 2 μL T4 ligase (NEB®, M0202), 2 μL PaqCI (NEB® R0745), 2 μl PaqCI activator (NEB® S0532), 0.1 pmol of each plasmid, 0.3 pmol of annealed oligos, and water

(5) A programmable ribozyme for RNA signal transductionNature CommunicationsJanuary 10, 2026Mandy Yu Theng Lim, Chermaine Tan, Charannya Sozheesvari Subhramanyam, Shi Jie Teo, Louis DeFalco, Samuel Kevin Pasaribu, Chong Hui Koh, Dheeraj Rayamajhi, Jieying Chi, Shengnan Li, et al.obtain the linearised DNA template for IVT, the sequence-verified plasmid was digested with PaqCI (#R0745L, New England Biolabs, USA) and gel-isolated with the FavorPrep™ GEL/PCR Purification Kit. Ribozyme

(6) Biology of host-dependent restriction-modification in prokaryotesEcoSal PlusDecember 16, 2025Brian P. Anton, Robert Blumenthal, James B. Eaglesham, Iwona Mruk, Richard J. Roberts, Shuang-yong Xu, Peter R. Weigele, Elisabeth A. Raleighthe common requirement for a “bystander oligonucleotide” (e.g., the PaqCI activator provided with NEB R0745, and discussion of two-site enzymes [see https://www.neb.com/en-us/nebinspired-blog/using-paqci-for-golden-gate-assembly]). An

(7) High-resolution spatial mapping of cell state and lineage dynamics in vivo with PEtracerScienceOctober 16, 2025Luke W. Koblan, Kathryn E. Yost, Pu Zheng, William N. Colgan, Matthew G. Jones, Dian Yang, Arhan Kumar, Jaspreet Sandhu, Alexandra Schnell, Dawei Sun, et al.backbones can then be used for a second cloning step using the Type IIS restriction enzyme PaqCI (NEB, R0745L) for the final 24-mer assembly. The overhangs for the 8-mer assembly are the same for each of 8-mer

(8) Systematic discovery of CRISPR-boosted CAR T cell immunotherapiesNatureSeptember 24, 2025Paul Datlinger, Eugenia V. Pankevich, Cosmas D. Arnold, Nicole Pranckevicius, Jenny Lin, Daria Romanovskaia, Moritz Schaefer, Francesco Piras, Anne-Christine Orts, Amelie Nemc, et al.CROPseq_REV_019 (Supplementary Table 1i), followed by DpnI digestion. PCR products were digested with PaqCI (NEB, R0745S), heat inactivated and cleaned with the Monarch PCR & DNA Cleanup Kit (NEB, T1030). The purified PCR

(9) Comprehensive profiling of activity and specificity of RNA-guided transposons reveals opportunities to engineer improved variantsNucleic Acids ResearchSeptember 23, 2025Seong Guk Park, Jung-Un Park, Esteban Dodero-Rojas, John A Bryant, Geetha Sankaranarayanan, Elizabeth H KelloggTnsC fragment using SapI and T4 DNA ligase. Next, barcode fragments were assembled using PaqCI (NEB, R0745L) and T4 DNA ligase. The barcoded TnsC library was digested with PacI (NEB, R0547L) and sent to the Hartwell

(10) Model-guided design of microRNA-based gene circuits supports precise dosage of transgenic cargoes into diverse primary cellsCell SystemsJune 18, 2025Kasey S. Love, Christopher P. Johnstone, Emma L. Peterman, Stephanie Gaglione, Michael E. Birnbaum, Kate E. Gallowaytranscriptional units were then combined with a backbone vector and a 200-bp spacer via PaqCI (NEB, R0745L) Golden Gate cloning to construct the lentiviral and PiggyBac integration vectors. The lentiviral backbone

(11) Small molecule- and cell contact-inducible systems for controlling expression and differentiation in mouse embryonic stem cellsDevelopmentJune 1, 2025Sarah S. Soliman, Devan H. Shah, Hana El-Samad, Zara Y. Weinberget al., 2024) via a PaqCI reaction following the manufacturer's instructions (New England Biolabs, R0745S). The Ngn2 plasmid was Addgene plasmid #34999 (deposited by Malin Parmar, Lund University, Sweden).

(12) Viral delivery of an RNA-guided genome editor for transgene-free germline editing in ArabidopsisNature PlantsApril 22, 2025Trevor Weiss, Maris Kamalu, Honglue Shi, Zheng Li, Jasmine Amerasekera, Zhenhui Zhong, Benjamin A. Adler, Michelle M. Song, Kamakshi Vohra, Gabriel Wirnowski, et al.site were phosphorylated and annealed. Then, the annealed double-stranded DNA was used in a PaqCI (R0745S) golden-gate reaction with the pMK435 ccdb intermediate vector (Supplementary Table 8). The golden-gate

(13) Effective recognition of double-stranded RNA does not require activation of cellular inflammationSci AdvApril 11, 2025Karolina Drazkowska, Julia Cieslicka, Michal Kitowicz, Anna Pastucha, Lukasz Markiewicz, Wiktoria Szymanek, Krzysztof Goryca, Tomasz Kowalczyk, Dominik Cysewski, Andreas R. Bausch, et al.(36, 39) with some modifications. A pJET-based construct linearized with PacQI (New England Biolabs, R0745) restrictase served as a template for in vitro transcription. The reaction mixture contained RNA Pol

(14) Antigenic drift expands influenza viral escape pathways from recalled humoral immunityImmunityMarch 11, 2025Daniel P. Maurer, Mya Vu, Aaron G. Schmidtsuch that the whole plasmid could be assembled in a one-pot golden gate reaction with PaqCI (NEB, #R0745S). Due to repeated regions, we found it necessary to propagate this plasmid in NEB Stable E. coli (NEB,

(15) Evaluation of Cas13d as a tool for genetic interaction mappingNat CommunFebruary 14, 2025Ghanem El Kassem, Jasmine Hillmer, Michael BoettcherVCR1L were selected to avoid recombination48. The pMB1 plasmid was digested with PaqCI and NheI (NEB, R0745S, R0131) and the linear plasmid was purified from a 1% agarose gel. Gene fragments with Gibson assembly

(16) An in vivo CRISPR screen in chick embryos reveals a role for MLLT3 in specification of neural cells from the caudal epiblastDevelopmentFebruary 1, 2025Ashley R. G Libby, Tiago Rito, Arthur Radley, James BriscoeFrance) and RARα sequences were PCR amplified with primers adding PaqCI cut sites (New England Biolabs, R0745S), cut via PaqCI and ligated into an EF1alpha-mScarlet backbone (Zhang et al., 2024 preprint). Clones

(17) Improved gene editing and fluorescent-protein tagging in Aspergillus nidulans using a Golden Gate-based CRISPR-Cas9 plasmid systemWellcome Open ResearchOctober 17, 2024Domenico Modaffari, Aimée Finlayson, Yuyang Miao, Edward W. J. Wallace, Kenneth E. SawinUK). CRISPR-Cas9 plasmids containing sgRNA spacer sequences were assembled by Golden Gate Reaction using PaqCI (#R0745, New England Biolabs, USA). 60bp-long complementary oligonucleotides were annealed by mixing 2.5 µL

(18) Pythia: Non-random DNA repair allows predictable CRISPR/Cas9 integration and gene editingbioRxivSeptember 27, 2024Thomas Naert, Taiyo Yamamoto, Shuting Han, Melanie Horn, Phillip Bethge, Nikita Vladimirov, Fabian F. Voigt, Joana Figueiro-Silva, Ruxandra Bachmann-Gagescu, Fritjof Helmchen, et al.Linearization was performed by overnight digest at 37C° of 10 μg of donor plasmid using 20 Units of PaqCI (R0745, NEB) in 1x rCutSmart buffer (B6004S, NEB). Complete linearization was ensured via classical agarose gel electrophoresis

(19) Model-guided design of microRNA-based gene circuits supports precise dosage of transgenic cargoes into diverse primary cellsbioRxivJuly 13, 2024Kasey S. Love, Christopher P. Johnstone, Emma L. Peterman, Stephanie Gaglione, Kate E. Gallowaytranscriptional units were then combined with a backbone vector and a 200-bp spacer via PaqCI (NEB, R0745L) Golden Gate cloning to construct the lentiviral and PiggyBac integration vectors. The lentiviral backbone

(20) An integrated technology for quantitative wide mutational scanning of human antibody Fab librariesNature CommunicationsMay 10, 2024Brian M. Petersen, Monica B. Kirby, Karson M. Chrispens, Olivia M. Irvin, Isabell K. Strawn, Cyrus M. Haas, Alexis M. Walker, Zachary T. Baumer, Sophia A. Ulmer, Edgardo Ayala, et al.plus 3 μL PaqCI activator (diluted from 20 μM stock 1:4 in 1X T4 DNA ligase buffer) (NEB; catalog # R0745L) - for ligation of barcode to VH. The reaction was subjected to 60 cycles of 37 °C for one minute followed

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PaqCI from New England Biolabs

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