Fig 1: FE-SEM images at ×10,000 magnification of slides coated with (A) WSM, (B) PEG-WSM, (C) BMP2, and (D) PEG-BMP2. Mineralization features below 1 µm can be seen as a “dusting of snow” in both non-PEGylated proteins BMP2 and WSM. PEGylation inhibits this type of mineralization in BMP2 but not in WSM as they are present in the PEG-WSM slide but not on the PEG-BMP2 slide. All four samples revealed crystals with an acicular habit. Main scale bars are 2 μm; inset scale bars are 1 μm. Error bars show standard deviation. Asterisks denote significant difference between indicated samples (p < 0.05). Note No mineralization was observed in PEGylated DMP1 samples.
Fig 2: ALP-stained images of PEGDA hydrogels incorporating patterned PEGylated WSM (A) and BMP2 (C) were thresholded for intensity and overlaid with pattern (B,D). Mineralization associated with the overlaid patterns were quantified (D). Graph indicates percentage of stained cells associated with patterned PEGylated WSM. Scale bars are 100 µm. Error bars represent standard deviation.
Fig 3: FE-SEM micrographs of mineralization on glass slides patterned with PEGylated proteins. (A) A nylon mesh photomask was used to pattern (B) PEGylated Nacre WSM, (C) BMP2, (D) DMP-1, (E) BSP, and (F) n16N. Scale bars are 100 µm.
Supplier Page from Thermo Fisher Scientific for Recombinant Human BMP-2 Protein