Recombinant Human EGF Protein PHG0311L from Thermo Fisher Scientific

Product Specs
ItemRecombinant Human EGF Protein
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPHG0311L
Size100 µg
ApplicationsControl, ELISA, Functional assay, Immunohistochemistry, Western Blot
Concentration1 mg/mL
FormatPBS with no preservative
TypeRecombinant
SpeciesHuman
Molecule NameHuman EGF
NCBI Full Gene Nameepidermal growth factor
NCBI Gene AliasesHOMG4, URG
Storage-20° C, Avoid Freeze/Thaw Cycles

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Glioblastoma Cells Expressing Oncogenic EGFR Release Multiple Extracellular Vesicle Subpopulations Positive or Negative for EGFRJ Extracell BiolApril 7, 2026Elham Pishavar, Fereshteh Shojaei-Ghahrizjani, Brian Meehan, Nadim Tawil, Cristiana Spinelli, Dongsic Choi, Fang Cheng Wong, Mahsa Jalali, Janusz Rakas spheres in a media containing DMEM‐F12 (GIBCO, 11320033) supplemented with 100 µg/ml EGF (GIBCO, PHG0311L), 100 µg/ml FGF (GIBCO, PHG0261), 0.2% Heparin (STEMCELL, 07980), 1X B27 serum‐free supplement (GIBCO,

(2) Understanding Glioblastoma Dynamics Using 3D Organoids and Engineered Extracellular MatrixAdvanced ScienceMarch 28, 2026Autumn McManis, Charles Ashley Jimenez, Abha Shirolkar, Syed Raza ur Rehman, Sumana Mallick, Malea Murphy, Akhilesh K. Gaharwar, Irtisha Singhstock; Thermo Fisher Scientific, Cat. No. 12587010), 20 ng/mL human EGF recombinant protein (Cat. No. PHG0311L), 20 ng/mL human FGF‐basic recombinant protein (Cat. No. PHG0261), 0.2 µg/mL hydrocortisone (from 50

(3) Ferroptosis in Meibomian Gland Progenitor Cells Contributes to Pathogenesis of Meibomian Gland DysfunctionInvestigative Ophthalmology & Visual ScienceJanuary 7, 2026Yuli Guo, Sai Luo, Wansui Li, Xuechun Wang, Wenxin Sun, Wei Zha, Rongrong Zhang, Dandong Liu, Mingyan Wei, Houjian Zhang, et al.TryplE Express (12605036) were purchased from Gibco (Waltham, MA, USA). Recombinant human EGF (hEGF; PHG0311L) and LipidTox (H34477) were purchased from Invitrogen (Waltham, MA, USA). Bovine pituitary extract (BPE;

(4) Cadherin-1-mediated communication at the leader-follower boundary controls mouse breast tumor organoid collective migrationCell ReportsDecember 23, 2025Vasilios A. Morikis, Diego Barra Avila, Alessandra DiMauro, Yanyang Cao, Priscilla Y. Hwang, Amit Pathak, Gregory D. Longmore,Thermo Fisher,Cat# 28908 Tween 20,Sigma-Aldrich,Cat# P9416 FGF,Sigma-Aldrich,Cat# F0291 EGF,Gibco,Cat# PHG0311L SDF1,Sigma,Cat# SRP3276 "Insulin, Transferrin, Sodium Selenite (ITS)",Gibco,Cat# 41400-045 Bradford

(5) Organoid platinum-resistance model identifies KRT17 as a biomarker of targeted therapy in ovarian canceriScienceDecember 19, 2025Juliane Reichenbach, Juliana Schmid, Sophia Hierlmayer, Tingyu Zhang, Ilaria Piga, Sophia Geweniger, Jonas Fischer, Aarushi Davesar, Nemanja Vasovic, Anca Chelariu-Raicu, et al.BMP2,"GibcoTM, Thermo Scientific™",Cat# PHC7146 Human recombinant EGF,"GibcoTM, Thermo Scientific™",Cat# PHG0311L Human recombinant Heregulin beta-1,Peprotech,Cat# 100-03 "Milk powder, 500 g",Carl Roth,Cat#68514-61-4 Mowiol®

(6) Functional requirements of the liver isoform of phosphofructokinase-1 in breast cancer cell migrationJournal of Cell ScienceDecember 1, 2025Heather L. Hansen, Bradley A. Webbwith 50 ng/ml human recombinant epidermal growth factor (Thermo Fisher Scientific, Rockfield IL, USA; PHG0311L) for 5 min, washed three times in PBS and fixed in 4% paraformaldehyde for 30 min. Cell were permeabilized

(7) Trypanosoma cruzi infection-induced changes in cardiac microvascular endothelial cell morphology and functionJournal of Medical MicrobiologyNovember 13, 2025Lyndsey N. Gisclair, Douglas A. Johnston(Gibco Cat. 10372019) supplemented with 10 ng ml−1 recombinant human epidermal growth factor (Gibco Cat. PHG0311L), 1 ug ml−1 hydrocortisone (Sigma Cat. H0888-1G), 10 mM glutamine (Gibco Cat. 25030081), 10% fetal bovine

(8) Assessing the Independent and Combined Effects of Hyperosmolarity and Inflammatory Stress on Human Corneal Epithelial CellsInvest Ophthalmol Vis SciOctober 28, 2025Deeksha Prasad, Mohammad Gufran Siddiqui, Mahi Al Ahmad Dar, Rohini Sonar, Swati Singh, Pragnya Rao Donthineni, Sayan Basu, Vivek Singhsupplemented with 10% fetal bovine serum (FBS; S11150H, R&D), 10 ng/mL human epidermal growth factor (EGF; PHG0311L; Thermo Fisher, USA), 5 µg/mL insulin (12585014; Thermo Fisher, USA), and 1% antibiotic–antimycotic

(9) Understanding proneural–mesenchymal transition using patient-derived glioma stem-like cell (GSC) organoids and engineered extracellular matrixbioRxivSeptember 24, 2025Autumn McManis, Charles Ashley Jimenez, Abha Shirolkar, Syed Raza Ur ur Rehman, Sumana Mallick, Malea Murphy, Akhilesh K Gaharwar, Irtisha Singhstock; Thermo Fisher Scientific, Cat. No. 12587010), 20 ng/mL human EGF recombinant protein (Cat. No. PHG0311L), 20 ng/mL human FGF-basic recombinant protein (Cat. No. PHG0261), 0.2 μg/mL hydrocortisone (from 50

(10) NRCAM variant defined by microexon skipping is a targetable cell surface proteoform in high-grade gliomasCell ReportsAugust 26, 2025Priyanka Sehgal, Ammar S. Naqvi, Makenna Higgins, Jiageng Liu, Kyra Harvey, Julien Jarroux, Taewoo Kim, Berk Mankaliye, Pamela Mishra, Grace Watterson, et al.Cat#12587–010), N2 supplement (Thermo Fisher Cat#175020–48), EGF recombinant protein (Thermo Fisher Cat#PHG0311L), FGF recombinant protein (Peprotech Cat#100–18B), Heparin (Sigma Cat#H3149), 2 mmol/L L-glutamine,

(11) β-catenin functions as a molecular adapter for disordered cBAF interactionsMolecular CellAugust 21, 2025Yuen San Chan, Qinyu Gao, Sarah A. Robinson, Wenzhi Wang, Ruzena Filandrova, Lisa-Maria Weinhold, Mario Loeza Cabrera, Miao Zhang, Chandra Shekar R. Ambati, Antonio M. Lerario, et al.(Sigma-Aldrich #I0516), 8.4 ng/mL cholera toxin (Sigma-Aldrich #C8052), 10 ng/mL epidermal growth factor (Gibco #PHG0311L), 24 μg/mL adenine (Sigma-Aldrich #A2786) and 1% Pen/Strep as previously reported58. HEK 293T cells

(12) EFTUD2 Regulates Cortical Morphogenesis via Modulation of Caspase‐3 and Aifm1 Splicing PathwaysAdvanced ScienceAugust 1, 2025Liping Chen, Ying Li, Yan Yu, Mingze Cai, Hao Li, Minghe Huang, Guochao Yang, Jiageng Guo, Huailin Wang, Zhihong Song, et al.containing 2% B27 (Gibco, 17504044), 20 ng mL−1 recombinant human epidermal growth factor (EGF) (Gibco, PHG0311L), and 20 ng mL−1 basic fibroblast growth factor (bFGF) (Gibco, 13256029)] on ice. After removing the

(13) Radioprotective effects of asiaticoside and asiatic acid in neural stem cells derived from human stem cells from apical papilla through increasing dose-reduction factor and their lowering effects on SH-SY5Y cell viabilityPLoS OneJune 4, 2025Taweesak Tangrodchanapong, Apisada Jiso, Pimolpun Changkaew, Suphalak Khachonkham, Amarin Thongsuk, Tarinee Chodchavanchai, Phisit Khemawoot, Nisarat Ruangsawasdi, Permphan Dharmasaroja, Anupong Thongklam SongsaadTechnologies), 1% Antibiotic-Antimycotic, 20 ng/mL bFGF, and 20 ng/mL epidermal growth factor (EGF, PHG0311L, Gibco, Life Technologies) in ultra-low attachment multiple-well plate (3473, Corning, NY, USA) for

(14) The Influence of Pathological Extracellular Matrix on the Biological Properties of Stem Cells: Possible Hints for Cell Transplantation Therapies in Spinal Cord InjuryInternational Journal of Molecular SciencesApril 23, 2025Giuseppe Alastra, Corinne Quadalti, Vito Antonio Baldassarro, Alessandro Giuliani, Luciana Giardino, Laura Calzàgrowth factors were added: 10 ng/mL bFGF (Gibco, cat no. PHG0024) and 10 ng/mL EGF (Gibco, cat no. PHG0311L). The resulting cells were plated at a seeding density of 10 cells/µL in a T25 flask (Corning, New York,

(15) Dynamic interaction of MYC enhancer RNA with YEATS2 protein regulates MYC gene transcription in pancreatic cancerEMBO ReportsApril 11, 2025Jayita Roy, Aniket Kumar, Shouvik Chakravarty, Nidhan K Biswas, Srikanta Goswami, Anup MazumderGlucose,Invitrogen,11885084 FBS,Gibco,16000044 Pen/Strep,Gibco,15140122 puromycin,Invitrogen,A1113803 EGF,Gibco,PHG0311L recombinant TNF-α,Abclonal,30021400 TRIzol reagent,"Ambion, Life Technologies",15596026 Verso cDNA Synthesis Kit

(16) Tolerability and Efficacy of Topical Methotrexate in Ocular Surface DiseaseCureusMarch 21, 2025Nathan A Seto, Calvin W Wong, Xiaowen Lu, Mitchell A Watsky, Richard W Yee(ThermoFisher Scientific, Waltham, MA), and 5 ng/mL recombinant human epidermal growth factor (rEGF) (Gibco, #PHG0311L). Primary human corneal stromal cell cultures (HCSC) were also established from de-identified donor

(17) Enhanced kinase translocation reporters for simultaneous real-time measurement of PKA, ERK, and calciumJournal of Biological ChemistryMarch 1, 2025Shang-Jui Tsai, Yijing Gong, Austin Dabbs, Fiddia Zahra, Junhao Xu, Aleksander Geske, Michael J. Caterina, Stephen J. GouldATP (Sigma, A26209), Forskolin (Cayman Chemical, 11018), H89 (MedChemExpress, HY-15979A), EGF (Gibco, PHG0311L), VX-11e (Selleckchem, S7709), leptomycin B (Sigma Aldrich, L2913), and Phorbol 12-myristate 13-acetate

(18) A Reporter Gene Assay for Measuring the Biological Activity of PEGylated Recombinant Human Growth HormoneMoleculesFebruary 3, 2025Shaowang Hu, Xiaoming Zhang, Yi Li, Jing Li, Yingwu Wang, Chenggang Liangantibiotics, lipofectamine 3000, recombinant human Interleukin-2 (IL-2, PHC0027), IL-4 (200-04), EGF (PHG0311L), and IGF-1 (RP-10931) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). The Nb2-11 cell

(19) The small GTPase MRAS is a broken switchNat CommunJanuary 14, 2025Gabriela Bernal Astrain, Regina Strakhova, Chang Hwa Jo, Emma Teszner, Ryan C. Killoran, Matthew J. Smithstimulation was carried out by replacing media in the 6-well dish with DMEM containing 50 ng/ml EGF (Gibco, PHG0311L) for 5 mins. Cells were washed with PBS and lysed in RIPA buffer (20 mM Tris-HCl (pH 7.5), 1 mM EDTA,

(20) Mesothelial cell responses to acute appendicitis or small bowel obstruction reactive ascites: Insights into immunoregulation of abdominal adhesionPLoS OneJanuary 8, 2025Melissa A. Hausburg, Kaysie L. Banton, Christopher D. Cassidy, Robert M. Madayag, Carlos H. Palacio, Jason S. Williams, Raphael Bar-Or, Rebecca J. Ryznar, David Bar-OrNutrient Mixture F12 (Thermo Fisher, 11765054) with 10 ng/ml epidermal growth factor (EGF) (Gibco, PHG0311L), 400 ng/ml hydrocortisone (Sigma, #H0888), GlutaMAX (Thermo Fisher, 35050061), and Penicillin-Streptomycin

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Recombinant Human EGF Protein from Thermo Fisher Scientific

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Recombinant Human EGF Protein from Thermo Fisher Scientific

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