Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) followed by Streptavidin-HRP (SB Cat. No. 7105-05) and chemiluminescent detection.
Streptavidin-HRP
ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG1κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
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