Proteinase K (20 mg/ml) 10012S from Cell Signaling Technology

Product Specs
ItemProteinase K (20 mg/ml)
CompanyCell Signaling Technology
Price $38.00 Inquire
Catalog Number10012S
Research AreaRelated Products
Size100 µl
ApplicationsChIP, C&R

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Cytoplasmic mRNA decay by the antiviral nuclease RNase L promotes transcriptional repressionCell ReportsMarch 24, 2026Xiaowen Mao, Sherzod Tokamov, Felix Pahmeier, Azra Lari, Jinyi Xu, Eva Harris, Britt GlaunsingerAldrich,T4509-100MG HPDP-Biotin,Thermo Fisher Scientific,PI21341 pAG-MNase,Cell Signaling,40366S proteinase K,Cell Signaling,10012S RNase A,Thermo Fisher Scientific,EN0531 4-Thio-UTP,Jena Bioscience,NU-1156S T7 RNA polymerase,NEB,"M0251L

(2) Phosphoproteomics of aged insulin-resistant bone identifies P70S6K phosphorylation of AFF4 as a gene-specific transcriptional regulatorNature CommunicationsDecember 31, 2025Mriga Dutt, Luoping Liao, Hani Jieun Kim, Ronnie Blazev, Audrey Chan, Hitesh Kore, Ayenachew Bezawork-Geleta, Li Dong, Isela Sarahi Rivera, Natalie K. Y. Wee, et al.37 °C. The samples were further digested with 40 µg of Proteinase K (Cell Signaling Technologies; #10012) and de-crosslinked overnight at 65 °C. DNA fragments were purified using ChIP DNA Clean & Concentrator

(3) NF-κB-dependent GR cistrome redistribution recruits GR to inflammatory genes but correlates with lesser glucocorticoid-mediated repressioniScienceNovember 25, 2025Mahmoud M. Mostafa, Amandah Necker-Brown, Alex Gao, Andrew J. Thorne, Akanksha Bansal, Lucy Swift, Annika M. Maj, Sarah K. Sasse, Pina Colarusso, Anthony N. Gerber, et al.Methanol-free",Thermo Scientific,043368 Dynabeads Protein G,Thermo,10004D Proteinase K,Cell Signaling,10012S RNase A,Cell Signaling,7013S BlockAid Blocking Solution,Invitrogen,B10710 Image-iT FX Signal Enhancer,Invitrogen,I36933 ProLong Gold Antifade Mountant with DAPI

(4) MYC promotes group 3 medulloblastoma cell proliferation and alleviates ROS-induced cell death by upregulating transketolaseActa Neuropathol CommunJune 28, 2025Shizun Wang, Dan Zhang, Chunlong Wang, Yuqin Liurotation. The beads were washed three times with wash buffer. 6 µL of 5 M NaCl and 2 µL of Proteinase K (#10012) were added, and the samples were incubated at 65 °C for 2 h to reverse the cross-links and obtain DNA

(5) CDH1 loss remodels gene expression and lineage identity in human mammary epithelial cellsbioRxivJune 25, 2025Maggie Musick, Chinasa A. Ufondu, Carmen E. Rowland, Joseph L. Sottnik, Madeleine T. Shackleford, Camryn S. Nesiba, Julie H. Ostrander, Matthew J. Sikora,Thermo Fisher,Cat # 62249 DNA Extraction Buffer,Cell Signaling,Cat # 42015 Proteinase K,Cell Signaling,Cat # 10012 RNase A,Cell Signaling,Cat # 7013 Critical Commercial Assays,Critical Commercial Assays,Critical Commercial

(6) Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript ASTSci AdvMay 9, 2025Rui Li, Kaveh Daneshvar, Xinjie Ji, Michelle Pleet, Grace Igbinosun, Mohd Shameel Iqbal, Fatah Kashanchi, Alan C. Mullen, Fabio Romerio(Cell Signaling Technology, 7013) and incubated at 37°C for 30 min. Then, 2 μl of Proteinase K (Cell Signaling Technology, 10012) was added, and the sample was incubated at 65°C for 2 hours to overnight. After incubation, chromatin

(7) Protocol to study the genomic profile of histone lactylation with CUT&RUN assay in tumor-associated macrophagesSTAR ProtocApril 11, 2025Alessandra De Leo, Alessio Ugolini, Filippo VegliaReagent,Volume Extraction Buffer (Cell Signaling#42015),197.5 μL Proteinase K (Cell Signaling #10012),2 μL RNAse A (Cell Signaling #7013),0.5 μL DNA extraction buffer

(8) Removal of toxic steroidal glycoalkaloids and bitterness in tomato is controlled by a complex epigenetic and genetic networkSci AdvFebruary 21, 2025Feng Bai, Mengbo Wu, Wei Huang, Weijie Xu, Yikui Wang, Yang Zhang, Zhenhui Zhong, Yiguo Hong, Julien Pirrello, Mondher Bouzayen, et al.cross-linking. Afterward, they were incubated with Proteinase K (50 mg/ml, Cell Signaling Technologies; #10012S) at 65°C for 3 hours. The chromatin that was immunoprecipitated was dissolved in water for qPCR analysis. Y2H

(9) Protocol for transcriptomic and epigenomic analyses of tip-like endothelial cells using scRNA-seq and ChIP-seqSTAR ProtocJanuary 10, 2025Shintaro Funasaki, Yuri Miyamura, Shunsuke Kamei, Akhinur Rahman, Masaya Yamazaki, Shingo Usuki, Keiichiro Yasunaga, Yorifumi Satou, Hiroto Ohguchi, Takashi MinamiGelatin,638-00781 Formaldehyde,Nacalai,09154-85 Accumax,Innovative Cell Technologies,AM105 Ethanol,FUJIFILM Wako,057-00451 Proteinase K,Cell Signaling Technology,#10012 MNase,Cell Signaling Technology,#10011 Glycine solution (10×),Cell Signaling Technology,#7005 Nuclease-free water

(10) Enhancers and genome conformation provide complex transcriptional control of a herpesviral geneMolecular Systems BiologyNovember 19, 2024David W Morgens, Leah Gulyas, Xiaowen Mao, Alejandro Rivera-Madera, Annabelle S Souza, Britt A Glaunsinger,Cell Signaling,40366S RNAase A,Thermo,EN0531 Spike-in DNA,Cell Signaling,40366S Protease K,Cell Signaling,10012 NEXT UltraII DNA Library Prep,NEB,E7645L Unique Dual Index UMI Adaptors,NEB,E7395S AMPure XP,Beckman,A63880 Hi-C+

(11) Phase-separated super-enhancers confer an innate radioresistance on genomic DNAJournal of Radiation ResearchJuly 22, 2024Koki Matsumoto, Dini Kurnia Ikliptikawati, Kei Makiyama, Kako Mochizuki, Maho Tobita, Isao Kobayashi, Dominic Chih-Cheng Voon, Keesiang Lim, Kazuma Ogawa, Ikuo Kashiwakura, et al.the proteins, the mixture was diluted in water, and Proteinase K (Cell Signaling Technology (CST), #10012) was added to a final concentration of 250 μg/ml, along with 187.5 mM NaCl (CST, #7010), and incubated

(12) A distal enhancer guides the negative selection of toxic glycoalkaloids during tomato domesticationNature CommunicationsApril 3, 2024Feng Bai, Peng Shu, Heng Deng, Yi Wu, Yao Chen, Mengbo Wu, Tao Ma, Yang Zhang, Julien Pirrello, Zhengguo Li, et al.reversing crosslink, and then incubating with 50 mg/mL Proteinase K (Cell Signaling Technologies; #10012 S) at 65 °C for 3 h. The immunoprecipitated chromatin was recovered, dissolved in water for subsequent

(13) Targeting PERK-ATF4-P21 axis enhances the sensitivity of osteosarcoma HOS cells to Mppα-PDTAging (Albany NY)February 5, 2024Shenxi Zhong, Ye Zhang, Hai Mou, Changchun Jian, Qiu Huang, Yunsheng OuChIP-grade protein G agarose beads (9007) were used to harvest DNA–protein complexes. RNase A (No 7013) and proteinase K (10012) were used to treat the precipitates. All reagents used in the ChIP assays were purchased from Cell Signaling Technology

(14) Histone lactylation driven by mROS-mediated glycolytic shift promotes hypoxic pulmonary hypertensionJournal of molecular cell biologyApril 20, 2023Chen J, Zhang M, Liu Y, Zhao S, Wang Y, Wang M, Niu W, Jin F, Li Z.from beads. The cross-linking of ChIP-DNA and input sample was reversed by proteinase K incubation (10012, Cell Signaling Technology) at 65°C for 2 h and purified with purification columns. The ChIP-DNA was

(15) RAD18 opposes transcription-associated genome instability through FANCD2 recruitmentPLoS geneticsDecember 1, 2022Wells JP, Chang EY, Dinatto L, White J, Ryall S, Stirling PC.future use. For DRIP, DNA samples were treated with RNAse A (CST#7013) followed by proteinase K (CST#10012) overnight at 65°C. DNA samples were purified with Cell Signaling spin columns (#14209S). DNA concentration

(16) Integrative analysis and prediction of human R-loop binding proteinsG3 Genes|Genomes|GeneticsJuly 29, 2022Arun Kumar, Louis-Alexandre Fournier, Peter C Stirlingfurther use. DNA samples for DRIP were treated with RNAse A (CST#7013) followed by proteinase K (CST#10012) overnight at 65°C. DNA samples were purified with Cell Signaling spin columns (#14209S). DNA concentration

(17) Loss of NFE2L3 protects against inflammation-induced colorectal cancer through modulation of the tumor microenvironmentOncogeneMarch 10, 2022James Saliba et al.Eluates were reverse cross‐linked by adding 6 μL 5 mol/L NaCl and 2 μL Proteinase K (Cell Signaling, 10012), and incubated for 2 h at 65 °C. DNA was extracted and purified using Spin Columns (Cell Signaling,

(18) Functional antagonism of chromatin modulators regulates epithelial-mesenchymal transitionScience advancesFebruary 1, 2021Serresi M, Kertalli S, Li L, Schmitt MJ, Dramaretska Y, Wierikx J, Hulsman D, Gargiulo G.cross-linking was performed by adding 6 μl of 5 M NaCl and 2 μl of proteinase K (Cell Signaling Technology, #10012) and incubation for 2 hours at 65°C. The DNA was purified by means of spin columns and eluted in 50

(19) α-Actinin 4 Links Vasopressin Short-Term and Long-Term Regulation of Aquaporin-2 in Kidney Collecting Duct CellsFrontiers in physiologyJanuary 1, 2021Ho CH, Yang HH, Su SH, Yeh AH, Yu MJ.then transferred to a new tube to reverse the crosslink with 200 mM NaCl and 2 μl proteinase K (Cat. 10012, Cell Signaling Technologies, United States) at 65°C for 2 h. The chromatin DNA was then purified with

(20) CD1d1 intrinsic signaling in macrophages controls NLRP3 inflammasome expression during inflammationScience advancesOctober 1, 2020Cui S, Wang C, Bai W, Li J, Pan Y, Huang X, Yang H, Feng Z, Xiang Q, Fei L, et al.eluted from antibody/protein G magnetic beads and reversed cross-links with protein K (2 μl per sample; #10012, CST). DNA was extracted and purified using spin columns (#10010, CST). qPCR was performed with special

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Proteinase K (20 mg/ml) from Cell Signaling Technology

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Proteinase K (20 mg/ml) from Cell Signaling Technology

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