Immunohistochemical staining of perfusion-fixed frozen brain sections with Anti-D₁ Dopamine Receptor Antibody (#ADR-001), (1:100), (green). A. DRD1 appears in the soma and dendrites of cortical pyramidal neurons in layer 5. B. The same section was stained for Calbindin D28k (red), a marker of interneurons. C. Merging of the two images demonstrates that localization of DRD1 is restricted to pyramidal neurons. DAPI is used as the counterstain.

Immunohistochemical staining of perfusion-fixed frozen brain sections with Anti-D₁ Dopamine Receptor Antibody (#ADR-001), (1:100), (green). A. DRD1 appears in the striatal matrix (star). B. The same section was stained for Calbindin D28k (red), a marker of interneurons. C. Merging of the two images demonstrates that localization of DRD1 is restricted to the matrix. DAPI is used as the counterstain.

1,2. Anti-D₁ Dopamine Receptor Antibody (#ADR-001), (1:200).
3,4. Anti-D₁ Dopamine Receptor Antibody, preincubated with D1 Dopamine Receptor Blocking Peptide (#BLP-DR001).