Human IL-23 Protein (His & AVI Tag), Biotinylated CT048-H27H-B from Sino Biological, Inc.

Product Specs
ItemHuman IL-23 Protein (His & AVI Tag), Biotinylated
CompanySino Biological, Inc.
Price $298.00Supplier Page View Company Product Page
Catalog NumberCT048-H27H-B
TypeRecombinant protein
Molecule NameIL12B & IL23A
SpeciesHuman
NCBI Full Gene Nameinterleukin 12B, interleukin 23 subunit alpha
NCBI Gene AliasesIL-23A, CLMF, IL-23, IL23P19, NKSF, NKSF2, CLMF2, P19, SGRF, IMD29, IMD28, IL-12B
FormatSupplied as sterile 50 mM Tris, 100 mM Nacl, 10%glycerol, 0.02% Tween 80, 10% Trehalose, pH 8.5
Conjugate/TagC-AVI, His
Source/Expression SystemHEK293 Cells
Molecular WeightThe recombinant human IL23 complex (IL23A&IL12B) consists of 517 amino acids and predicts a molecular mass of 57.6 kDa
Purity> 95 % as determined by SDS-PAGE
Activity1.Immobilized Recombinant Human IL23 Protein (His & AVI Tag), Biotinylated (Cat: CT048-H27H-B ) at 2 µg/mL (100 µl/well) on streptavidin precoated (2 µg/mL, 100 µL/well) can bind Recombinant Human IL23R / IL23 Receptor Protein (ECD, Fc Tag) (Cat: 13840-H02H), the EC50 is 8-30 ng/mL. 2.Immobilized Recombinant Human IL23 Protein (His & AVI Tag), Biotinylated (Cat: CT048-H27H-B)at 2 µg/mL (100 µL/well) can bind Recombinant Human IL12RB1 Protein (ECD, Fc Tag) (Cat: 11674-H02H), the EC50 is 1-3 ng/mL (Routinely tested). 3.Loaded Biotinylated Recombinant Human IL-23 Protein, His & AVI Tag (Cat. No. CT048-H27H-B) on SA Biosensor, can bind Recombinant Human IL-23 Receptor Protein, hFc Tag (Cat. No. 13840-H02H) with an affinity constant of 65.7 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 4.Loaded Biotinylated Recombinant Human IL-23 Protein, His & AVI Tag (Cat. No. CT048-H27H-B) on SA Biosensor, can bind Recombinant Human IL-12 R beta 1/IL12RB1 Protein, hFc Tag (Cat. No. 11674-H02H) with an affinity constant of 60.3 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 5.Loaded Anti-IL-12 & IL-23 (P40)(Research Grade Ustekin*mab Biosimilar) on proA Biosensor, can bind Biotinylated Recombinant Human IL-23 Protein, His & AVI Tag (Cat. No. CT048-H27H-B) with an affinity constant of 0.551nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 6.Loaded Anti-IL-23 Antibody (P19) (Research Grade Risankiz*mab Biosimilar) on proA Biosensor, can bind Biotinylated Recombinant Human IL-23 Protein, His & AVI Tag (Cat. No. CT048-H27H-B) with an affinity constant of 1.25nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 7.Anti-IL-23 Antibody (P19) (Research Grade Risankiz*mab Biosimilar) captured on Protein A chip can bind Biotinylated Recombinant Human IL-23 Protein, His & AVI Tag (Cat. No. CT048-H27H-B) with an affinity constant of 0.319 nM as determined in an SPR assay (Biacore 8K) (Routinely tested). 8.Recombinant Human IL-23 Receptor Protein, hFc Tag (Cat. No. 13840-H02H) captured on Protein A chip can bind Biotinylated Recombinant Human IL-23 Protein, His & AVI Tag (Cat. No. CT048-H27H-B) with an affinity constant of 0.669 nM as determined in an SPR assay (Biacore 8K) (Routinely tested)
Size20 µg