Hi-T4 DNA Ligase M2622S from New England Biolabs

Product Specs
ItemHi-T4 DNA Ligase
CompanyNew England Biolabs
Price $70.00 Inquire
Catalog NumberM2622S
Size20000 units
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Loss of LLGL1 Elevates EGFR/RAS/MAPK Signaling and Remodels EMT Markers in Huh-7 Hepatocellular Carcinoma CellsInternational Journal of Molecular SciencesMarch 24, 2026Gökhan Yıldız, Soner Karabulut, Tuba Dincer, Bayram Toramanoligonucleotides were generated by annealing complementary sgRNA pairs with T4 polynucleotide kinase (Cat. No: M2622; New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions. The annealed oligonucleotide

(2) LZTR1 Loss Reduces Vimentin Expression and Motility in Hep3B Hepatocellular Carcinoma CellsInternational Journal of Molecular SciencesFebruary 15, 2026Gökhan Yıldız, Soner Karabulut, Umit Uzun, Onur Obut, Vahap Eldem, Tuba Dinçer, Bayram Toramanoligonucleotides were generated by annealing complementary sgRNA pairs using T4 polynucleotide kinase (Cat. No: M2622; New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions. Annealed oligonucleotide

(3) Simultaneous capture of single cell RNA-seq, ATAC-seq, and CRISPR perturbation enables multiomic screens to identify gene regulatory relationshipsCell Reports MethodsDecember 15, 2025Kaivalya Shevade, Yeqing Angela Yang, Kevin Feng, Karl Mader, Volkan Sevim, Jacob Parsons, Gunisha Arora, Hasnaa Elfawy, Rachel Mace, Scot Federman, et al.,ThermoFisher Scientific,Cat# C1430 Ethidium Homodimer-1,ThermoFisher Scientific,Cat# E1169 Hi-T4 ligase,NEB,Cat# M2622S Kapa HiFi HotStart ReadyMix,Roche,Cat# KK2601 ,, Critical commercial assays,Critical commercial assays,Critical

(4) Sequencing-free whole-genome spatial transcriptomics at single-molecule resolutionCellNovember 26, 2025Yubao Cheng, Shengyuan Dang, Yuan Zhang, Yanbo Chen, Ruihuan Yu, Miao Liu, Shengyan Jin, Ailin Han, Samuel Katz, Siyuan Wang100 μL of Hi-T4 DNA ligation mixture (containing 4000 U of Hi-T4™ DNA Ligase (New England Biolabs; M2622L), 1 μM splint oligo, 1 mM DTT, 1 mM ATP, 0.2 mg/mL BSA, 40 U of MRI, and 1× Hi-T4 DNA ligase buffer)

(5) Replication competition drives the selective mtDNA inheritance in Drosophila ovaryCell ReportsSeptember 23, 2025Cheng Zhang, Zhe Chen, Hansong Ma, Hong Xu,New England Biolabs,Cat# R0146L BglII,New England Biolabs,Cat# R0144M Hi T4 DNA ligase,New England Biolabs,Cat# M2622L T4 DNA ligase,New England Biolabs,Cat# M0202L Lambda exonuclease,New England Biolabs,Cat# M0262L 20×SSC

(6) Protocol for inducible expression of small non-coding RNAs using a tet-on system in Saccharomyces cerevisiaeSTAR ProtocolsSeptember 19, 2025Regina Nostramo, Anita K. HopperQ5 high-fidelity DNA polymerase,New England Biolabs,Cat. # M0491S Hi-T4 DNA ligase,New England Biolabs,Cat. # M2622S SalI-HF,New England Biolabs,Cat. # R3138S Chloroform,Fisher Scientific,Cat. # AAJ67241K2 "BD Difco

(7) Breaking the cancer code: a novel DNA minicircle to disable STAT3 in ovarian cancer cells SKOV3Frontiers in PharmacologySeptember 15, 2025Adina-Gabriela Vasilescu, Andrei-Mihai Vasilescu, Livia Elena Sima, Natalia Baran, Ștefan-Eugen Szedlacsek(NEB; Cat no P0756L) to a final concentration of 100 μM and 3 U/µL of Hi-T4™ DNA Ligase (NEB, Cat no M2622S) to a final volume of 1 mL. The ligation reaction was incubated at RT for 16 h to achieve circularization. 2.4

(8) NEXN protects against vascular calcification by promoting SERCA2 SUMOylation and stabilizationNat CommunAugust 29, 2025Wenjie Guo, Wenjing Guo, Boliang Chen, Zexuan Lin, Zhuohua Wen, Jiamin Ye, Wei Feng, Xin Feng, Jianyun Yan, Pingzhen Yang, et al.plasmids were constructed by inverse PCR using pairs of nonoverlapping primers and Hi-T4 DNA Ligase (NEB, M2622S). Quantification of VSMCs and aorta calcification (calcium assay) The calcium content of VSMCs and

(9) An enzyme-based approach for highly efficient self-replication of DNA origami dimersProceedings of the National Academy of SciencesJuly 22, 2025Lei Zhang, Ruojie Sha, Paul Chaikindevelopment of complex artificial life systems. Materials and Methods Materials. Hi-T4™ DNA ligase (M2622S) was purchased from New England Biolabs. Agarose (A9539) was purchased from Sigma-Aldrich. DNA Synthesis

(10) Bidirectional regulation of glycoprotein nonmetastatic melanoma protein B by β-glucocerebrosidase deficiency in GBA1 isogenic dopaminergic neurons from a patient with Gaucher disease and parkinsonismbioRxivJune 28, 2025Chase Chen, Charis Ma, Richard Sam, Jens Lichtenberg, Tiffany Chen, Ying Hao, Ziyi Li, Isabelle Kowal, Kate Andersh, Yue Andy Qi, et al.sequence in the original plasmid through SpeI and MluI digestion and Hi-T4™ ligation (New England Biolabs, M2622). The HDR donor vectors used to revert GBA1 N370S to WT were built using pDONOR-tagBFP-PSM-EGFP (Addgene,

(11) Multiome Perturb-seq unlocks scalable discovery of integrated perturbation effects on the transcriptome and epigenomeCell SystemsJanuary 15, 2025Eli Metzner, Kaden M. Southard, Thomas M. NormanSIRLOIN oligo was replaced with a duplex oligo ligated into the digested product with Hi-T4 ligase (NEB #M2622) at RT for 1 hr. For each cloning reaction, assembled vectors were transformed into NEB Stable E. coli,

(12) The CD2v protein of African swine fever virus inhibits macrophage migration and inflammatory cytokines expression by downregulating EGR1 expression through dampening ERK1/2 activityVeterinary ResearchNovember 15, 2023Min Zhang, Lilei Lv, Huaye Luo, Hongming Cai, Lingxue Yu, Yifeng Jiang, Fei Gao, Wu Tong, Liwei Li, Guoxin Li, et al.lentiviral vector pLV-EF1a-IRES-Hygro (Addgene, Cat#:85134) using a Hi-T4 DNA Ligase Kit (NEB, Cat#: M2622S). Similarly, the sequence encoding EGFP (enhanced green fluorescent protein) was amplified with primers

(13) An efficient cloning method to expand vector and restriction site compatibility of Golden Gate AssemblyCell Reports MethodsAugust 22, 2023Masato Sorida, Roberto Bonasio,Thermo Scientific,SM1331 BsaI-HFv2,NEB,R3733S BbsI-HF,NEB,R3539S AgeI-HF,NEB,R3552S BamHI-HF,NEB,R3136S EcoRI-HF,NEB,R3101L HindIII-HF,NEB,R3104S NcoI-HF,NEB,R3193S NotI-HF,NEB,R3189S SalI-HF,NEB,R3138S SpeI-HF,NEB,R3133S XbaI,NEB,R0145S XhoI,NEB,R0146S XmaI,NEB,R0180S KpnI-HF,NEB,R3142S NdeI,NEB,R0111S Smal,NEB,R0141S BbsI-HF,NEB,R3539M T4 DNA Ligase,NEB,M0202L Hi-T4 DNA Ligase,NEB,M2622S 100 mM ATP,NEB,P0756S NEBuilder HiFi DNA Assembly Master Mix,NEB,E2621 LB Broth,Fisher Scientific,BP9723 Agar,Fisher

(14) Improved USER cloning for TALE assembly and its application to base editingPLoS OneAugust 4, 2023Jizeng Zhou, Jiaowei Wang, Fangbing Chen, Zhenpeng Zhuang, Min Chen, Yang Yang, Xian Luo, Chengcheng Tang, Xiaoqing Zhou, Yue Chi, et al.assembled with T4 DNA Ligase (New England Biolabs, #M0202) or Hi-T4 DNA Ligase (New England Biolabs, #M2622). In constructing all the single-guide RNA (sgRNA), two complementary oligonucleotides were synthesized

(15) Transient regulation of RNA methylation in human hematopoietic stem cells promotes their homing and engraftmentLeukemiaFebruary 1, 2023Wang X, Cooper S, Broxmeyer HE, Kapur R.After annealing, double-strand oligos were ligated into the shRNA expression plasmid by T4 ligase (NEB, M2622L). Well-constructed vectors were sent to Genewiz (South Plainfield, NJ, USA) for sequencing. Lentivirus

(16) HRD1-mediated METTL14 degradation regulates m(6)A mRNA modification to suppress ER proteotoxic liver diseaseMolecular cellDecember 16, 2021Wei J, Harada BT, Lu D, Ma R, Gao B, Xu Y, Montauti E, Mani N, Chaudhuri SM, Gregory S, et al.M7449 Q5 HiFi DNA Polymerase,New England Biolabs,Cat# M0491 T4 DNA ligase,New England Biolabs,Cat# M2622S Complete proteinase inhibitor cocktail,Roche,Cat# 11697498001 Protein G Magnetic Beads,New England Biolabs

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Hi-T4 DNA Ligase

Hi-T4 DNA Ligase from New England Biolabs

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Hi-T4 DNA Ligase from New England Biolabs

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