Description
Recombinant SARS-CoV-2 Spike Protein (RBD) consists of 234 amino acids (Arg319-Phe541) with C-terminal His-tag. It has an apparent molecular mass of ~25 kDa in SDS-PAGE. The concentration of protein was determined by BCA.
The SARS-CoV-2 glycosylated spike (S) protein highly exposed on the viral surface is a major determinant for virus binding and invasion into hose cells, which is a main target for neutralization antibody. The receptor-binding domain (RBD) in SARS-CoV-2 S protein is responsible for binding to human and bat angiotensin-converting enzyme 2 (ACE2) receptors