SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (HPLC-verified) 40592-V05H from Sino Biological, Inc.

Product Specs
ItemSARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (HPLC-verified)
CompanySino Biological, Inc.
Price $448.00Supplier Page View Company Product Page
Catalog Number40592-V05H
Molecule NameSpike
SpeciesSARS-CoV-2
FormatLyophilized from sterile PBS, pH 7.4
Source/Expression SystemHEK293 Cells
Molecular WeightThe recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag) consists of 457 amino acids and predicts a molecular mass of 51.5 kDa
Conjugate/TagC-mouse IgG1-Fc
Purity> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC
Activity1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2µg/mL (100µL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag)(40592-V05H), the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag) is 10-30 ng/mL. 2. Loaded Human ACE2 Protein (His tag) (Cat. No. 10108-H08H) on SA Biosensor, can bind SARS-CoV-2/2019-nCoV Spike/RBD Protein (RBD, mFc Tag) (Cat. No. 40592-V05H) with an affinity constant of 0.562 nM as determined by Octet RED System. 3.Loaded Recombinant Human ACE2 Protein, His Tag, Biotinylated (Cat. No. 10108-H27B-B) on SA Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (Cat. No. 40592-V05H) with an affinity constant of 1.75 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested)
TypeRecombinant protein
Size100 µg

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Sino Biological, Inc.

37 Industrial Blvd

Suite B

Paoli, PA 19301

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: sinobiological.com

(1) Oncolytic virotherapy potentiates chemo-PD-1 immunotherapy by engaging chemo-resistant bystander CD8+ T cellsJ Immunother CancerMarch 4, 2026Yang Yang, Junjian He, Texi Liang, Kaiyi Li, Yao Lin, Hairu Wang, Wenwen Xi, Shusen Ye, Ding Qiu, Chunyang Xie, et al.were immunized intranasally with two doses of 10 µg SARS-CoV-2 RBD protein-mFC tag (Sino Biological, 40592-V05H) conjugated to 10 µg CpG ODN 1826 (Invitrogen, tlrl-1826) at an interval of 2 weeks. Mouse tumor models B16-F10

(2) Ultrapotent SARS coronavirus-neutralizing single-domain antibodies that clamp the spike at its baseNat CommunMay 30, 2025Sieglinde De Cae, Inge Van Molle, Loes van Schie, Sophie R. Shoemaker, Julie Deckers, Nincy Debeuf, Sahine Lameire, Wim Nerinckx, Kenny Roose, Daria Fijalkowska, et al.SARS-CoV-2 Omicron BA.1 S protein (ACROBiosystems), mouse Fc-tagged SARS-CoV-2 RBD (Sino Biological, 40592-V05H), the SARS-CoV-2 spike S2 subunit (ACROBiosystems, S2NC52H5) or BSA. The coated plates were blocked

(3) An oncolytic virus delivering tumor-irrelevant bystander T cell epitopes induces anti-tumor immunity and potentiates cancer immunotherapyNature cancerJuly 1, 2024Chen X, Zhao J, Yue S, Li Z, Duan X, Lin Y, Yang Y, He J, Gao L, Pan Z, et al.were intranasally immunized with two doses of 10 μg SARS-CoV-2 RBD protein–mFC tag (Sino Biological, 40592-V05H) conjugated to 10 μg CpG ODN 1826 (Invitrogen, tlrl-1826) at an interval of 14 d. Construction of modified

(4) SARS-CoV-2 Infection Alters the Phenotype and Gene Expression of AdipocytesInternational journal of molecular sciencesFebruary 8, 2024Quaranta P, Scabia G, Storti B, Dattilo A, Quintino L, Perrera P, Di Primio C, Costa M, Pistello M, Bizzarri R, et al.and #170-6515, BioRad, Hercules, CA, USA), dilution: 1:3000.-Anti-S IgG rabbit monoclonal antibody (40592-V05H, Sino Biological, Beijing, China), dilution: 1:200.-Anti-N IgG mouse monoclonal antibody (#33717, Cell Signaling

(5) SARS-Cov-2 spike induces intestinal barrier dysfunction through the interaction between CEACAM5 and Galectin-9Frontiers in immunologyJanuary 1, 2024Luo Y, Zhang Z, Ren J, Dou C, Wen J, Yang Y, Li X, Yan Z, Han Y.intraperitoneally with 5μg recombinant spike-Fc containing the receptor binding domain (RBD) (Sino Biological, 40592-V05H, diluted in 200ul PBS) after 16 hours, while control group with 5μg control -Fc (Sino Biological, 10690-MNAH-100,

(6) Severe acute respiratory syndrome coronavirus 2 infection leads to Tau pathological signature in neuronsPNAS NexusSeptember 1, 2023Di Primio C, Quaranta P, Mignanelli M, Siano G, Bimbati M, Scarlatti A, Piazza CR, Spezia PG, Perrera P, Basolo F, et al.NGS-0.1% Triton in PBS solution. Primary antibodies are as follows: anti-S IgG rabbit monoclonal antibody (40592-V05H, Sino Biological); rabbit SARS-CoV-2 N antibody, 1:1,000 (GTX135357, GeneTex Irvine, CA, USA); anti-α-tubulin

(7) Neutralization, effector function and immune imprinting of Omicron variantsNatureSeptember 1, 2023Addetia A, Piccoli L, Case JB, Park YJ, Beltramello M, Guarino B, Dang H, de Melo GD, Pinto D, Sprouse K, et al.supernatants were diluted in PBS and mixed with SARS-CoV-2 Wu RBD mouse Fc-tagged antigen (Sino Biological, 40592-V05H) or with biotinylated BQ.1.1 or XBB.1 RBDs (Acrobiosystems) at a final concentration of 20 ng ml−1 and

(8) Deciphering intercellular signaling complexes by interaction-guided chemical proteomicsNature communicationsJuly 12, 2023Zheng J, Zheng Z, Fu C, Weng Y, He A, Ye X, Gao W, Tian R.100-39H; 0.1 μg/μL), PDGF-B (Peprotech, 100-14B; 0.1 μg/μL), SARS-CoV-2 RBD (mFc tag, Sino Biological, 40592-V05H; 0.5 μg/μL), porcine insulin (Aladdin, 12584-58-6; 0.5 μg/μL), or PLAU (His tag, Sino Biological, 10815-H08H-A;

(9) Therapeutic and vaccine-induced cross-reactive antibodies with effector function against emerging Omicron variantsbioRxiv : the preprint server for biologyFebruary 27, 2023Addetia A, Piccoli L, Case JB, Park YJ, Beltramello M, Guarino B, Dang H, Pinto D, Scheaffer S, Sprouse K, et al.

(10) Low Prevalence of SARS-CoV-2 Antibodies in Canine and Feline Serum Samples Collected during the COVID-19 Pandemic in Hong Kong and KoreaVirusesFebruary 20, 2023Yun Young Go, Maura Carrai, Yan Ru Choi, Christopher J. Brackman, Karina W. S. Tam, Pierra Y. T. Law, Fiona Woodhouse, Jane Gray, Ji Hun Kim, Joohyung Park, et al.SARS-CoV-2 Spike RBD-mFc Recombinant Protein (Sino Biological, Beijing, China, Sinobiological, cat. No. 40592-V05H) diluted in PBS buffer and refrigerated overnight at 4 °C. The next day, coated plates were blocked

(11) ApoE4 associated with severe COVID-19 outcomes via downregulation of ACE2 and imbalanced RAS pathwayJournal of translational medicineFebruary 9, 2023Chen F, Chen Y, Ke Q, Wang Y, Gong Z, Chen X, Cai Y, Li S, Sun Y, Peng X, et al.experiments were performed using Octet RED96 equipment (ForteBio). The recombinant RBD (SinoBiological, 40592-V05H) and recombinant human ACE2 proteins (SinoBiological, 10108-H02H) were captured with an anti-mIgG Fc

(12) Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escapeiScienceJanuary 20, 2023Marzi R, Bassi J, Silacci-Fregni C, Bartha I, Muoio F, Culap K, Sprugasci N, Lombardo G, Saliba C, Cameroni E, et al.supernatants were diluted in PBS and mixed with SARS-CoV-2 RBD mouse Fc-tagged antigen (Sino Biological, 40592-V05H, final concentration 20 ng/ml) and incubated for 30 min at 37°C. The mix was added for 30 min to ELISA

(13) Extended SARS-CoV-2 RBD booster vaccination induces humoral and cellular immune tolerance in miceiScienceDecember 22, 2022Feng-Xia Gao, Rui-Xin Wu, Mei-Ying Shen, Jing-Jing Huang, Ting-Ting Li, Chao Hu, Fei-Yang Luo, Shu-Yi Song, Song Mu, Ya-Nan Hao, et al.University (CQMU202104). Mice immunization strategy 50 μg RBD recombinant protein (Sinobiological: #40592-V05H) was dissolved in 100 μL PBS and then formulated in Freund’s complete adjuvant (Sigma: #9007-81-2) or

(14) A potent human monoclonal antibody with pan-neutralizing activities directly dislocates S trimer of SARS-CoV-2 through binding both up and down forms of RBDSignal Transduction and Targeted TherapyDecember 1, 2022Xiaofei Wang et al.at 4 °C for 30 min and then stained with biotin-conjugated SARS-CoV-2 RBD protein (Sino Biological, 40592-V05H) at 4 °C for 20 min. Next, PBMCs were stained with PE-Cy7-conjugated streptavidin (eBioscience), FITC-conjugated

(15) Host cell membrane proteins located near SARS-CoV-2 spike protein attachment sites are identified using proximity labeling and proteomic analysisThe Journal of biological chemistryNovember 1, 2022Kotani N, Nakano T, Kuwahara R.488-conjugated spike proteins Recombinant SARS-CoV-2 spike protein (S1-RBD) was purchased from Sino Biological (40592-V05H; S1-RBD-mouse Fc). Alexa Fluor 488-conjugated monovalent SARS-CoV-2 spike protein was prepared using

(16) Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escapebioRxivOctober 20, 2022Roberta Marzi, Jessica Bassi, Chiara Silacci-Fregni, Istvan Bartha, Francesco Muoio, Katja Culap, Nicole Sprugasci, Gloria Lombardo, Christian Saliba, Elisabetta Cameroni, et al.supernatants were diluted in PBS and mixed with SARS-CoV-2 RBD mouse Fc-tagged antigen (Sino Biological, 40592-V05H, final concentration 20 ng/ml) and incubated for 30 min at 37°C. The mix was added for 30 min to ELISA

(17) Bergamottin and PAP-1 Induced ACE2 Degradation to Alleviate Infection of SARS-CoV-2International journal of molecular sciencesOctober 19, 2022Li M, Zhang Y, Zeb A, Wu Y, Cheng L.ACE2/angiotensin-converting enzyme 2 protein (His Tag 10108-H08H) and SARS-CoV-2 Spike RBD-mFc recombinant protein (40592-V05H) were purchased from Chinese Sino Biological Science Company, Beijing, China. All other reagents of

(18) The N501Y Mutation of SARS-CoV-2 Spike Protein Impairs Spindle Assembly in Mouse OocytesReproductive sciences (Thousand Oaks, Calif.)October 1, 2022Liu H, Shi J, Fok KL, Chen H.

(19) Analysis of Serologic Cross-Reactivity Between Common Human Coronaviruses and SARS-CoV-2 Using Coronavirus Antigen MicroarraybioRxivSeptember 16, 2022Saahir Khan et al.,Sino Biological,N-(AA1541-1859)-His-C,40524-V08E Coronavirus,2019-nCoV,2019-nCoV,S1-RBD,,HEK293,Sino Biological,N-(AA)-mFc-C,40592-V05H Coronavirus,2019-nCoV,2019-nCoV,S1,,HEK293,Sino Biological,N-(AA)-His-C,40591-V08H Coronavirus,2019-nCoV,2019-nCoV,S1,,HEK293,Sino Biological

(20) Changes Over Time in COVID-19 Incidence, Vaccinations, Serum Spike IgG, and Viral Neutralizing Potential Among Individuals From a North American Gaming Venue: December 2020-August 2021Journal of Occupational & Environmental MedicineSeptember 1, 2022Adam V. Wisnewski, Linda Cantley, Julian Campillo Luna, Jian Liu, Richard F. Smith, Kelly Hager, Carrie A. RedlichSARS-CoV-2 (2019-nCoV) Spike Protein RBD (mFc-RBD) from Sino Biological (Chesterbrook, PA), catalog number 40592-V05H, at 2 μg/mL in 1 × PBS at 4°C overnight. Plates were blocked with 200 μL of 3% nonfat milk PBS for 60

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SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (HPLC-verified)

SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (HPLC-verified) from Sino Biological, Inc.

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Sino Biological, Inc.

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SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (HPLC-verified) from Sino Biological, Inc.

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