SARS-CoV-2 (2019-nCoV) Spike S1-Fc Recombinant Protein 40591-V02H from Sino Biological, Inc.

Product Specs
ItemSARS-CoV-2 (2019-nCoV) Spike S1-Fc Recombinant Protein
CompanySino Biological, Inc.
Price $498.00Supplier Page View Company Product Page
Catalog Number40591-V02H
Size100 µg
FormatLyophilized from sterile PBS, pH 7.4
Conjugate/TagC-human IgG1-Fc
Purity> 90 % as determined by SDS-PAGE
Activity1.Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2µg/mL (100µL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, Fc Tag)(40591-V02H), the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, Fc Tag) is 10-50 ng/mL. 2.Recombinant SARS-CoV-2 (2019-nCoV) Spike S1 Protein, Fc Tag (Cat. No. 40591-V02H) captured on Protein A chip can bind Recombinant Human ACE2 Protein, His Tag (Cat. No. 10108-H08H) with an affinity constant of 1.50 nM as determined in an SPR assay (Biacore 8K) (Routinely tested)
Molecule NameSpike
SpeciesSARS-CoV-2
Source/Expression SystemHEK293 Cells
Molecular WeightThe recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, Fc Tag) consists of 908 amino acids and predicts a molecular mass of 101.7 kDa
TypeRecombinant protein

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Sino Biological, Inc.

37 Industrial Blvd

Suite B

Paoli, PA 19301

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: sinobiological.com

(1) High-throughput multiplexed serology via the mass-spectrometric analysis of isotopically barcoded beadsNature biomedical engineeringJuly 1, 2025Drainas AP, McIlwain DR, Dallas A, Chu T, Delgado-González A, Baron M, Angulo-Ibáñez M, Trejo A, Bai Y, Hickey JW, et al.supernatant. Then, 100 μl of diluted spike S1 protein (2 ng μl−1) in CSM-T (Sino Biological, number 40591-V02H) was added to each well and incubated for 10 min at room temperature. The plate was centrifuged at 2,800

(2) Precise Control of Intracellular Trafficking and Receptor‐Mediated Endocytosis in Living Cells and Behaving AnimalsAdvanced ScienceDecember 1, 2024Shiau‐Chi Chen, Neng‐Jie Zeng, Grace Y. Liu, Hsien‐Chu Wang, Tzu‐Ying Lin, Yi‐Ling Tai, Chiao‐Yun Chen, Yin Fang, Yi‐Chien Chuang, Ching‐Lin Kao, et al.SARS‐CoV‐2 spike RBD protein (Spike subunit 1, Fc tagged recombinant protein; Sino Biological Inc.; 40591‐V02H) in serum‐free medium at 37 °C for 5 min. After this incubation, the unbound RBD protein was washed

(3) Broad Epitope Coverage of Therapeutic Multi-Antibody Combinations Targeting SARS-CoV-2 Boosts In Vivo Protection and Neutralization Potency to Corner an Immune-Evading VirusBiomedicinesMarch 13, 2024Roodink I, van Erp M, Li A, Potter S, van Duijnhoven SMJ, Smits M, Kuipers AJ, Kazemier B, Berkeveld B, van Geffen E, et al.and S1-S2-His, were purchased from Sino Biological (Beijing, China, cat.#’s 40591-V41H, 40591-V08H, 40591-V02H, 40591-V05H1, 40590-V02H, and 40589-V08B1, respectively). Spike protein trimers reflecting B.1.1.7,

(4) Sublingual Dissolving Microneedle (SLDMN)-Based Vaccine for Inducing Mucosal Immunity against SARS-CoV-2Advanced healthcare materialsOctober 1, 2023Kim Y, Park IH, Shin J, Choi J, Jeon C, Jeon S, Shin JS, Jung H.

(5) Analysis of Serologic Cross-Reactivity Between Common Human Coronaviruses and SARS-CoV-2 Using Coronavirus Antigen MicroarraybioRxivSeptember 16, 2022Saahir Khan et al.,Sino Biological,N-(AA)-His-C,40591-V08H Coronavirus,2019-nCoV,2019-nCoV,S1,,HEK293,Sino Biological,N-(AA)-Fc-C,40591-V02H Coronavirus,2019-nCoV,2019-nCoV,S1,,HEK293,Sino Biological,N-(AA)-Fc-C,40591-V05H1 Coronavirus,2019-nCoV,2019-nCoV,S2,,Insect

(6) Comparison of replicating and nonreplicating vaccines against SARS-CoV-2Science advancesAugust 26, 2022Mudrick HE, Massey S, McGlinch EB, Parrett BJ, Hemsath JR, Barry ME, Rubin JD, Uzendu C, Hansen MJ, Erskine CL, et al.

(7) A humanized nanobody phage display library yields potent binders of SARS CoV-2 spikePLOS ONEAugust 10, 2022Ying Fu et al.VHHs were carried out using RBD-mFc or S1-hFc recombinant proteins (Sino Biological, 40592-V05H and 40591-V02H, respectively) as analytes. VHHs were loaded at 10 μg/ml in kinetics buffer (PBS with 0.1% protease-free

(8) APOE interacts with ACE2 inhibiting SARS-CoV-2 cellular entry and inflammation in COVID-19 patientsSignal transduction and targeted therapyAugust 1, 2022Zhang H, Shao L, Lin Z, Long QX, Yuan H, Cai L, Jiang G, Guo X, Yang R, Zhang Z, et al.(Hitachi). Live-cell surface staining and STED 293T-ACE2 cells were incubated with Spike-Fc (SinoBiological, Cat#40591-V02H), Spike-Fc/APOE3, and Spike-Fc/APOE4 in culture medium for 1 h at 4 °C, respectively. Cells were washed

(9) A highly photostable and bright green fluorescent proteinNature biotechnologyJuly 1, 2022Hirano M, Ando R, Shimozono S, Sugiyama M, Takeda N, Kurokawa H, Deguchi R, Endo K, Haga K, Takai-Todaka R, et al.was immobilized onto an NTA sensor chip. Binding was evaluated by injecting S1-Fc (Sino Biological, 40591-V02H) solutions serially diluted (5–160 nM) with the running buffer (10 mM HEPES (pH 7.4), 150 mM NaCl, 50

(10) Non-propagative human parainfluenza virus type 2 nasal vaccine robustly protects the upper and lower airways against SARS-CoV-2iScienceDecember 17, 2021Ohtsuka J, Imai M, Fukumura M, Maeda M, Eguchi A, Ono R, Maemura T, Ito M, Yamayoshi S, Kataoka Y, et al.#420601 SARS-CoV-2 Spike Glycoprotein,GenScript,Cat #RP30020 SARS-CoV-2 Spike S1 Protein,Sino Biological Inc.,Cat #40591-V02H SARS-CoV-2 Spike Protein RBD,Sino Biological Inc.,Cat #40592-V02H Human ACE2 Protein,AcroBiosystems,Cat

(11) SARS-CoV-2 pseudovirus infectivity and expression of viral entry-related factors ACE2, TMPRSS2, Kim-1, and NRP-1 in human cells from the respiratory, urinary, digestive, reproductive, and immune systemsJournal of medical virologyDecember 1, 2021Zhang F, Li W, Feng J, Ramos da Silva S, Ju E, Zhang H, Chang Y, Moore PS, Guo H, Gao SJ.or SARS-CoV pseudovirus. Recombinant proteins used in this study included SARS-CoV-2 spike S1-hFc (40591-V02H, Sino biological), SARS-CoV-2 spike receptor binding domain (RBD) RBD-mFc (40592-V05H1, Sino biological),

(12) Common cardiac medications potently inhibit ACE2 binding to the SARS-CoV-2 Spike, and block virus penetration and infectivity in human lung cellsScientific reportsNovember 12, 2021Caohuy H, Eidelman O, Chen T, Liu S, Yang Q, Bera A, Walton NI, Wang TT, Pollard HB.

(13) Novel suction-based in vivo cutaneous DNA transfection platformScience advancesNovember 5, 2021Lallow EO, Jhumur NC, Ahmed I, Kudchodkar SB, Roberts CC, Jeong M, Melnik JM, Park SH, Muthumani K, Shan JW, et al.96-well, Corning, Corning, NY) were coated with SARS-CoV-2 spike S1-Fc recombinant protein (1.5 μg/ml; 40591-V02H, Sino Biological, Chesterbrook, PA) in 1× PBS and incubated overnight at 4°C. Plates were washed three

(14) Development of a Recombinant RBD Subunit Vaccine for SARS-CoV-2VirusesSeptember 26, 2021Sun YS, Zhou JJ, Zhu HP, Xu F, Zhao WB, Lu HJ, Wang Z, Chen SQ, Yao PP, Jiang JM, et al.Increase 10/300 GL, GE healthcare, Waltham, MA, USA) for the RBD recombinant protein. The S1-hFc protein (40591-V02H) was obtained from the Sino Biological Company (Beijing, China). 2.4. Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis

(15) Variable Induction of Pro-Inflammatory Cytokines by Commercial SARS CoV-2 Spike Protein Reagents: Potential Impacts of LPS on In Vitro Modeling and Pathogenic Mechanisms In VivoInternational journal of molecular sciencesJuly 14, 2021Ouyang W, Xie T, Fang H, Gao C, Stantchev T, Clouse KA, Yuan K, Ju T, Frucht DM.proteins were prepared by Sino Biological (Vendor #2; Beijing, China) under request. S1-Fc (Catalog # 40591-V02H, lot LC14AP1605), RBD-Fc (Catalog # 40592-V02H, lot LC14MC2602), S1-biotin (Catalog # 40591-V27HB, lot

(16) Identifying Primate ACE2 Variants That Confer Resistance to SARS-CoV-2Molecular biology and evolutionJune 25, 2021Bhattacharjee MJ, Lin JJ, Chang CY, Chiou YT, Li TN, Tai CW, Shiu TF, Chen CA, Chou CY, Chakraborty P, et al.were then incubated in 100 µl of PBS containing 120 ng of Fc-tagged spike S1 recombinant protein (Cat: 40591-V02H, Sino Biological) for 1 h. The cells were washed three times with PBS and incubated with rabbit anti-ACE2

(17) Structure and function analysis of a potent human neutralizing antibody CA521(FALA) against SARS-CoV-2Communications biologyApril 23, 2021Song D, Wang W, Dong C, Ning Z, Liu X, Liu C, Du G, Sha C, Wang K, Lu J, et al.with the mixture of recombinant RBD protein (Cat 40592-V05H, Sino Biological), Spike S1 protein (Cat 40591-V02H, Sino Biological) and Spike S2 protein (Cat 40590-V08B, Sino Biological) and boosted by 105 μg mixtures

(18) SARS-CoV-2 spike D614G change enhances replication and transmissionNatureApril 1, 2021Bin Zhou et al.at least 5 min. Polyhistidine-tagged S1 (40591-V08H) and S1(D614G) (40591-V08H3) and Fc-tagged S1 (40591-v02H) and S1(D614G) (40591-v02H3) proteins were purchased from Sino Biological, and diluted in reaction buffer

(19) Functional and genetic analysis of viral receptor ACE2 orthologs reveals a broad potential host range of SARS-CoV-2Proceedings of the National Academy of Sciences of the United States of AmericaMarch 23, 2021Liu Y, Hu G, Wang Y, Ren W, Zhao X, Ji F, Zhu Y, Feng F, Gong M, Ju X, et al.the recombinant protein, S1 domain of SARS-CoV-2 spike C-terminally fused with Fc (Sino Biological #40591-V02H; 1 μg/mL) at 4 °C for 30 min. After washing, cells were stained with goat anti-human IgG (H + L) conjugated

(20) SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interfaceNature communicationsMarch 11, 2021Bertoglio F, Meier D, Langreder N, Steinke S, Rand U, Simonelli L, Heine PA, Ballmann R, Schneider KT, Roth KDR, et al.S1-RBD, SARS-CoV-2, HEK293 (Sino Biological 40592-V08H); S1-humFc, SARS-CoV-2, HEK293 (Sino Biological 40591-V02H); S1S2, SARS-CoV-2, Baculovirus (Sino Biological 40589-V08B1); S2, SARS-CoV-2, Baculovirus (Sino Biological

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SARS-CoV-2 (2019-nCoV) Spike S1-Fc Recombinant Protein

SARS-CoV-2 (2019-nCoV) Spike S1-Fc Recombinant Protein from Sino Biological, Inc.

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Sino Biological, Inc.

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SARS-CoV-2 (2019-nCoV) Spike S1-Fc Recombinant Protein from Sino Biological, Inc.

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