Description
Siglec-10, also known as Sialic acid binding Ig like lectin 10 isoform 3, is a member of the immunoglobulin superfamily that is expressed on eosinophils, neutrophils, monocytes, and B cells. This protein bears an Immuno-receptor tyrosine based inhibitory motif within its cytoplasmic domain. It is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. Also, it binds to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules on the same cell. Recombinant human Siglec-10, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques