SplintR Ligase M0375L from New England Biolabs

Product Specs
ItemSplintR Ligase
CompanyNew England Biolabs
Price $475.00 Inquire
Catalog NumberM0375L
Size6250 units
ApplicationsFunctional Genomics
TypeEnzymes
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Super-enhancer-driven core transcription factor FOXP1 delays endothelial cell senescence via phase separation-mediated SESN3 activationTheranosticsJanuary 1, 2026Mao L, Liao ZF, Tang D, Qiu Y, Yang M, Li Y, Xie Y, Feng W, Zheng ZJ, Liu XM, et al.TD501), JQ-1 (MCE, HY7869), THZ1 (MCE, HY80013), SplintR buffer (NEB, B0375S), SplintR Ligase (NEB, M0375L), glycerol (Sigma-Aldrich, G5516), BSA (Sigma-Aldrich, B2064), EquiPhi29 buffer (Thermo Fisher Scientific,

(2) Massively Scalable Single-cell Multiomic Profiling of T Cell Repertoire with REFLEXResearch SquareDecember 23, 2025Matthew R. Skene, Zachary J. Thomson, Saransh N. Thomson, Saransh Ilcisin, Matthieu Landreau, Tyanna J. Landreau, Peter J. Stuckey, Micheal Wittig, Chase D. Keller, Troy R. Torgerson, et al.GEM master mix, 5 μL ATP (New England Biolabs, P0756L), 7.5 μL SplintR ligase [New England Biolabs, M0375L, 25,000 u/mL], and 3.3 μL RNase Inhibitor [Ambion, Superase-In, AM2696 20U/μL]. 49.2 μL ligation master

(3) REFLEX, a Novel Immune Profiling Assay, Combining TCR Repertoire and Multiome at Massively Scalable Single-cell Resolution to Catapult Exploration of T-cell Derived ImmunitybioRxivDecember 2, 2025Matthew R. Hart, Zachary J. Thomson, Saransh N. Kaul, Saskia Ilcisin, Matthieu Landreau, Tyanna J. Stuckey, Peter J. Wittig, Micheal Keller, Chase D. McCann, Troy R. Torgerson, et al.GEM master mix, 5 μL ATP (New England Biolabs, P0756L), 7.5 μL SplintR ligase [New England Biolabs, M0375L, 25,000 u/mL], and 3.3 μL RNase Inhibitor [Ambion, Superase-In, AM2696 20U/μL]. 49.2 μL ligation master

(4) ADAR1 as a Placental Innate Immune Rheostat Sustaining the Homeostatic Balance of Intrinsic Interferon Response at the Maternal‐Fetal InterfaceAdvanced ScienceNovember 1, 2025Xiaogang Chen, Xiangchao Xu, Jiahao Chen, Huiru Zhang, Wanshan Zheng, Wenzhe Yu, Xiaoqian Hu, Bin Cao37 °C for 12–16 h to facilitate ligation of the hybridized padlock probes using SplintR ligase (NEB, M0375L). Subsequently, rolling circle amplification was performed by incubating the slides in phi29 DNA polymerase

(5) Cassini: streamlined and scalable method for in situ profiling of RNA and proteinNature communicationsOctober 1, 2025Lapique N, Kim MT, Thom N, Nadaf NM, Pineda J, Macosko EZ.80 μl of PBS for 15 min at 37 °C, followed by one wash with 80 μl of 1X splintR ligase buffer (NEB M0375L) for 15 min at room temperature. The ligase solution was prepared on ice, with 1x splintR ligase buffer

(6) Deconvolving organogenesis in space and time via spatial transcriptomics in thick tissuesbioRxivSeptember 27, 2024Soichiro Asami, Chenshuo Yin, Luis A. Garza, Reza KalhorK (New England Biolabs, #P8107S), SplintR and SplintR ligase Reaction Buffer (New England Biolabs, #M0375L), Phi29 DNA Polymerase and Phi29 DNA Polymerase Reaction Buffer (New England Biolabs, #M0269L), Thermostable Inorganic Pyrophosphatase

(7) A bisulfite-assisted and ligation-based qPCR amplification technology for locus-specific pseudouridine detection at base resolutionNucleic Acids ResearchJune 10, 2024Xin Fang, Ruiqi Zhao, Yafen Wang, Mei Sun, Jin Xu, Shengrong Long, Jing Mo, Hudan Liu, Xiang Li, Fang Wang, et al.60°C for 1 min, 50°C for 1 min, and then 40°C for 6 min. Subsequently, 2 μl 1 U SplintR ligase (NEB, M0375L) was added to the former mixture. The final reaction mixture was incubated at 40°C for 60 min, denatured

(8) Quantification of tRNA m1A modification by templated-ligation qPCRRNAJune 1, 2024Wen Zhang, Hankui Chen, Marek Sobczyk, Daniel Krochmal, Christopher D. Katanski, Mahdi Assari, Amy Chen, Yichen Hou, Qing Dai, Tao Pangradual decreasing to 40°C. One microliter 10×SplintR ligase buffer and 0.2 µL SplintR ligase (NEB, M0375L) were then added to each ligation mixture and mixed (10 µL final ligation mixture). The ligation mixture

(9) DNA methylation regulates RNA m6A modification through transcription factor SP1 during the development of porcine somatic cell nuclear transfer embryosCell ProliferationMay 1, 2024Meng Zhang, Yanhui Zhai, Xinglan An, Qi Li, Daoyu Zhang, Yongfeng Zhou, Sheng Zhang, Xiangpeng Dai, Ziyi Limin. Subsequently, added Bst 2.0 DNA polymerase (0.01 U) (M0537l, NEB, USA), Splint ligase (0.5 U) (M0375l, NEB, USA), and ATP (10 nM) (P0756S, NEB, USA) with a total volume of 3 μL. The reaction mixture was

(10) Stromal cells-specific retinoic acid determines parturition timing at single-cell and spatial-temporal resolutioniScienceOctober 20, 2023Hui Zhao, Yang Wang, Hui Xu, Meng Liu, Xinmei Xu, Sijing Zhu, Zhao Liu, Han Cai, Yinan Wang, Jinhua Lu, et al.for denaturation and at 45°C for 120 min for hybridization. SplintR ligase (PBCV-1 DNA Ligase, NEB, M0375L) was added to ligate the hybridized padlock probes for 12-16 hours at 25°C. Phi 29 (Φ29) polymerase

(11) Dissecting mammalian spermatogenesis using spatial transcriptomicsCell reportsNovember 2, 2021Chen H, Murray E, Sinha A, Laumas A, Li J, Lesman D, Nie X, Hotaling J, Guo J, Cairns BR, et al.,New England Biolabs,Cat#: N0447L NxGen® RNase Inhibitor,Lucigen,Cat#: 30281 SplintR Ligase,New England Biolabs,Cat#: M0375L Terra PCR Direct Polymerase Mix,Takara Bio.,Cat#: 639271 Critical commercial assays,Critical commercial

(12) METTL3/METTL14 Transactivation and m⁶A-Dependent TGF-β1 Translation in Activated Kupffer CellsCellular and molecular gastroenterology and hepatologyJanuary 1, 2021Feng Y, Dong H, Sun B, Hu Y, Yang Y, Jia Y, Jia L, Zhong X, Zhao R.of enzyme mixture containing 0.01 U Bst 2.0 DNA polymerase (M0537l; NEB, USA), 0.5 U SplintR ligase (M0375l; New England Biolabs, Ipswich, MA), and 10 nmoL adenosine triphosphate (P0756S; New England Biolabs).

(13) Detection of Individual RNA in Fixed Cells and Tissues by Chromogenic ISHBIO-PROTOCOLFebruary 5, 2020Meng Jiang, Chen Lin, Rongqin Ke(Thermo Scientific, catalog number: R0182), store at -20 °C 22. SplintR Ligase, 25 U/µl (NEB, catalog number: M0375L), store at -20 °C 23. phi29 DNA Polymerase, 10 U/µl (Thermo Scientific, catalog number: EP0094), store

(14) SplintQuant: a method for accurately quantifying circular RNA transcript abundance without reverse transcription biasRNASeptember 1, 2019Vanessa Conn, Simon J. Connoligonucleotides, 100 nmol, desalted (Integrated DNA Technologies)SplintR Ligase (New England Biolabs, Cat. #M0375L)Extreme Thermostable Single-Stranded DNA Binding Protein (ET SSB) (MCLAB, Cat. # ETSSB-200)5X oligonucleotide

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SplintR Ligase

SplintR Ligase from New England Biolabs

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New England Biolabs

Citations (14)

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SplintR Ligase from New England Biolabs

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