BsiWI-HF R3553S from New England Biolabs

Product Specs
ItemBsiWI-HF
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR3553S
Size300 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Synthetic circRNAs employ IRES activity for translation in cells and in cell-free translation systemsbioRxivMarch 30, 2026Philipp Koch, Frederic S. W. Arendrup, Chaehee Lim, Samyukta Narayanan, Amina Adam, Massimiliano Clamer, Anders H. Lund, Chun-Kan Chen, Kathrin LeppekPolymerase, M0493S). The plasmid backbone was digested using EcoRI-HF (NEB, R3101S) and BsiWI-HF (NEB, R3553S) restriction enzymes according to manufacturer’s instructions. The Gibson Assembly reaction was performed

(2) In vivo site-specific engineering to reprogram T cellsNatureMarch 18, 2026William A. Nyberg, Pierre-Louis Bernard, Wayne Ngo, Charlotte H. Wang, Jonathan Ark, Allison Rothrock, Gina M. Borgo, Gabriella R. Kimmerly, Jae Hyung Jung, Vincent Allain, et al.a second PCR for overlap extension. The final assembled amplicon was digested using BsiWI-HF (NEB, R3553S) and SbfI-HF (NEB, R3642S) and ligated into the pITR2-Rep2-dead(GFP)Cap6 backbone using T4 DNA ligase

(3) A non-catalytic role for RFC in PCNA-mediated processive DNA synthesisCellFebruary 19, 2026Gabriella N. L. Chua, Emily C. Beckwitt, Victoria Miller-Browne, Olga Yurieva, Dan Zhang, Bryce J. Katch, Nina Y. Yao, John W. Watters, Kaitlin Abrantes, Ryogo Funabiki, et al.,New England Biolabs,Cat# R3131S BsrGI-HF,New England Biolabs,Cat# R3575S BsiWI-HF,New England Biolabs,Cat# R3553S BsaI-HF®v2,New England Biolabs,Cat# R3733S T4 DNA Ligase,New England Biolabs,Cat# M0202S SphI-HF,New England Biolabs

(4) CRISPRi screening in cultured human astrocytes uncovers distal enhancers controlling genes dysregulated in Alzheimer’s diseaseNature NeuroscienceDecember 18, 2025Nicole F. O. Green, Gavin J. Sutton, Javier Pérez-Burillo, Juli Wang, Samuel Bagot, Hannah G. Danon, Kieran Walsh, Akira Gokool, Samantha A. Miles, Guang Yang, et al.(Supplementary Table 10). The gBlock and CROP-seq-opti-GFP were digested with BsiWI (New England Biolabs, R3553S). The digested plasmid was dephosphorylated with Shrimp Alkaline Phosphatase (New England Biolabs, M0371S),

(5) Circular RMST cooperates with lineage-driving transcription factors to govern neuroendocrine transdifferentiationCancer CellMay 12, 2025Mona Teng, Jiacheng Guo, Xin Xu, Xinpei Ci, Yulin Mo, Yakup Kohen, Zuyao Ni, Sujun Chen, Wang Yuan Guo, Martin Bakht, et al.cat. #M0201L). Backbone plasmids were digested with AgeI (NEB, cat. #R3101S) and EcoRI (NEB, cat. #R3553S). Annealed oligos and digested plasmids were then annealed with T4 DNA ligase (NEB, cat. #M0202S). Annealed

(6) tRNA lysidinylation is essential for the minimal translation system in the Plasmodium falciparum apicoplastEMBO ReportsMarch 20, 2025Rubayet Elahi, Sean T PriggeInc.,Cat # R0564S BspEI,New England Biolabs Inc.,Cat # R0540S BsiWI,New England Biolabs Inc.,Cat # R3553S BsaI,New England Biolabs Inc.,Cat # R3733S T4 DNA ligase,New England Biolabs Inc.,Cat # M0202 In-Fusion,Clontech

(7) Mutability and hypermutation antagonize immunoglobulin codon optimalityMolecular CellJanuary 16, 2025Joshua J.C. McGrath, Juyeon Park, Chloe A. Troxell, Jordan C. Chervin, Lei Li, Johnathan R. Kent, Siriruk Changrob, Yanbin Fu, Min Huang, Nai-Ying Zheng, et al.,New England Biolabs,Cat#R3552S SalI-HF,New England Biolabs,Cat#R3138S BsiWI-HF,New England Biolabs,Cat#R3553S XhoI,New England Biolabs,Cat#R0146S Critical commercial assays,Critical commercial assays,Critical

(8) Identification of an autotransporter peptidase of Rickettsia rickettsii responsible for maturation of surface exposed autotransportersPLOS PathogensJuly 31, 2023Adam M. Nock, Karin Aistleitner, Tina R. Clark, Dan Sturdevant, Stacy Ricklefs, Kimmo Virtaneva, Yixiang Zhang, Naila Gulzar, Neelam Redekar, Amitiva Roy, et al.isolated using a GeneJet PCR Purification Kit (Thermoscientific, K0702), digested with BsiWi-HF (NEB, R3553S) and BssHII (NEB, R0199S), then re-purified using the GeneJet PCR Purification Kit per manufacturer’s

(9) Molecular basis for antiviral activity of two pediatric neutralizing antibodies targeting SARS-CoV-2 Spike RBDiScienceJanuary 20, 2023Chen Y, Prévost J, Ullah I, Romero H, Lisi V, Tolbert WD, Grover JR, Ding S, Gong SY, Beaudoin-Bussières G, et al.Technologies,N/A LD655-CoA,Lumidyne Technologies,N/A SgrAI,New England Biolabs,Cat # R0603S BsiWI-HF,New England Biolabs,Cat # R3553S AgeI-HF,New England Biolabs,Cat # R3552S NheI-HF,New England Biolabs,Cat # R3131S Octet® Amine Reactive

(10) Neutralizing Human Antibodies against Severe Acute Respiratory Syndrome Coronavirus 2 Isolated from a Human Synthetic Fab Phage Display LibraryInternational journal of molecular sciencesFebruary 15, 2021Kim YJ, Lee MH, Lee SR, Chung HY, Kim K, Lee TG, Kim DY.USA): for VH, EcoRI (Cat. R3101S) and NheI (Cat. R3131S); for VL, XhoI (Cat. R0146S) and BsiWI (Cat. R3553S). The digestion products were separated on a 1.2% agarose gel, and the single band was purified with

(11) Targeted removal of epigenetic barriers during transcriptional reprogrammingNature CommunicationsDecember 1, 2019Valentin Baumann, Maximilian Wiesbeck, Christopher T. Breunig, Julia M. Braun, Anna Köferle, Jovica Ninkovic, Magdalena Götz, Stefan H. StrickerdCas9-VP64-T2A-Blast plasmid, the plenti-dCas9-VP64-Blast (Addgene Plasmid 61425) was digested with BsiWI (NEB, R3553S) and BsrGI (NEB, R0575S). The dCas9-VP64 version from the dCas9-VP64-Hygro plasmid was amplified using

(12) A Refined Protocol for Identifying Citrulline-specific Monoclonal Antibodies from Single Human B Cells from Rheumatoid Arthritis Patient MaterialBIO-PROTOCOLSeptember 5, 2019khaled Amara, Lena Israelsson, Ragnhild Stålesen, Peter Sahlström, Johanna Steen, Vivianne Malmström, Caroline Grönwallwith restriction enzymes 1. AgeI-HF (NEB, catalog number: R3552L) 2. BsiWI-HF (NEB, catalog number: R3553S) 3. SalI-HF (NEB, catalog number: R3138S) 4. XhoI-HF (NEB, catalog number: R0146S) 5. 70% Ethanol (in

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BsiWI-HF

BsiWI-HF from New England Biolabs

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New England Biolabs

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BsiWI-HF from New England Biolabs

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