Nucleoside Digestion Mix M0649S from New England Biolabs

Product Specs
ItemNucleoside Digestion Mix
CompanyNew England Biolabs
Price $129.00 Inquire
Catalog NumberM0649S
Size50 reactions
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A pair of DNA glucosyltransferases elevate counter-defense in bacteriophage T4Nucleic Acids ResMay 28, 2026Luis Ramirez-Chamorro, Frédéric Bonhomme, Anton Lukas Ipsen Wolff, Mathieu Stouf, François Lecointe, Marcel Hollenstein, Mart Krupovic, Marianne De Paepe, Yuvaraj Bhoobalan-Chittymicrogram of total DNA was digested in 20-μl reaction volume using the NEB Nucleoside digestion mix (#M0649S) according to the manufacturer’s instructions. Analysis of global levels of dC, 5-mdC, 5-hmdC, 5(α)-gmdC,

(2) Translational repression of viral RNAs supports persistent arbovirus infection in mosquitoesPLoS BiolApril 28, 2026Marc Talló-Parra, Mireia Puig-Torrents, Gemma Pérez-Vilaró, Sol Ribó Pons, Juana Díezamount of extracted tRNA was digested with 1 μl of the Nucleoside Digestion Mix (New England BioLabs, M0649S) and the mixture was incubated at 37 ºC for 1 h. Samples (15 ng) were analyzed using an Orbitrap Eclipse Tribrid mass spectrometer

(3) Ligand-Induced Opening of a Cryptic Pocket in METTL14ACS Bio & Med Chem AuApril 15, 2026Ivan Corbeski, Rajiv Kumar Bedi, Christian M. Matter, Fiona Stamm, Elena Bochenkova, Marcin Herok, Michael J. Hartshorn, Amedeo Caflischto nucleosides and dephosphorylated in a one-pot reaction using 0.5 μL of nucleoside digestion mix (M0649S, NEB) in 20 μL of total reaction volume for 16 h at 37 °C. The samples were used for UPLC-MS/MS analysis

(4) Z-Calling: a tool for A/Z (2,6-diaminopurine) base calling and dZ-DNA detection using PacBio HiFi readsCommunications BiologyMarch 13, 2026Bo Wu, Ying Chen, Yan Zhou, Longjian Niu, He-Xu Chen, Yating Li, Jia-Yong Zhong, Suwen Zhao, Wei Chi, Yan Zhang, et al.and pRS425-ApDUF550 for dZTP biosynthesis)9 were enzymatically digested by Nucleoside Digestion Mix (#M0649S, New England Biolabs, Ipswich, USA) and separated on Amplicon Ultra-0.5 mL 3 K centrifugal filters (MilliporeSigma,

(5) Tissue-specific role of dTrmO in threonine decoding during Drosophila melanogaster developmentBiological ResearchFebruary 7, 2026Deborah Cuper, Valentina Muñoz-Madrid, Rodolfo Moreno, Francisca Brown, Yueh-Lin Tsai, Álvaro Glavic\begin{document}$$\:^\circ\:$$\end{document}C overnight using a Nucleoside Digestion Mix (NEB, Cat #M0649S). The digested RNAs were subsequently injected without prior purification on an Agilent 1290 Infinity

(6) Global discovery of RNA modifications and functional analysis of m5C methylome in cyanobacteriaJournal of Biological ChemistryFebruary 1, 2026Gaoxiang Cao, Mingtian Ling, Jiao Zhan, Jian Lin, Li Yuan, Mingkun Yang, Feng GeRNA modifications by LC–MS/MS Total RNA samples were digested using Nucleoside Digestion Mix Kit (M0649S; NEB) at 37 °C for 1 h and analyzed by an ACQUITY UPLC system coupled to a Xevo TQ MS (Waters). The

(7) Automated mapping of DNA replication fork progression in human cells with ForkMLNature CommunicationsJanuary 23, 2026Victoria Rojat, Diletta Ciardo, Alan Tourancheau, Florence Proux, Etienne Jean, Jean-Michel Arbona, Benjamin Audit, Gael A. Millot, Frédéric Bonhomme, Paola B. Arimondo, et al.nanopore sequencing or digested into nucleosides using the Nucleoside Digestion Mix (New England Biolabs #M0649S). Each of the six conditions was analysed once, with a single LC-MS injection per sample. Quantification

(8) Complexoform-restricted covalent TRMT112 ligands that allosterically agonize METTL5Nature Chemical BiologyJanuary 8, 2026F. Wieland Goetzke, Steffen M. Bernard, Cheng-Wei Ju, Jonathan Pollock, Kristen E. DeMeester, Jacob Gross, Gabriel M. Simon, Chuan He, Bruno Melillo, Benjamin F. Cravattonce with 1 ml of HPLC-grade water. The eluted RNA was then digested by nucleoside digestion mix (NEB, M0649S). The digested nucleosides were subjected to LC–MS analysis. The experiment with the 60-nt probe was

(9) Human MutLα activates methylpurine DNA glycosylase to induce alkylation damage cytotoxicitybioRxivJanuary 1, 2026Mohamed E. Ashour, Ellissa Krekeler, Monika Chandan Bhowmik, Ning Tsao, Carlos Herrera-Montávez, Miaw-Sheue Tsai, Holland Kantar, Manal Zaher, Roberto Galletto, Nima Mosammaparast69506). After extraction, 2 μg of genomic DNA was digested with 3 μL of Nucleoside Digestion Mix (NEB, M0649S) in a total volume of 50 μL at 37°C overnight. The digested sample was filtered with a 0.22 μM filter

(10) Beyond RNA modification: a novel role for tRNA modifying enzyme in oxidative stress response and metabolismNucleic Acids ResDecember 11, 2025Louna Fruchard, Claudia Sudol, Caroline Rouard, Aurore Treffkorn-Maurau, Léo Hardy, Julia Bos, Magalie Duchateau, Quentin Giai Gianetto, Mariette Matondo, Frédéric Bonhomme, et al.digested into single nucleosides using the Nucleoside digestion mix from New England BioLabs (Cat No. M0649S). Each 10 µL aliquot of RNA, diluted to 100 ng/µL in ultrapure water, was combined with 1 µL of enzyme

(11) Amyloid-mediated RNA uptake by sperm for embryonic deliverybioRxivDecember 11, 2025William S. Mallen, Nataliya Zabello, Susan Brenner-Morton, Simone Sidoli, Isabella D. Pirozzolo, Feng-Xia Liang, Joseph Sall, Jason Yin, Priyanka Mathews, Guoan Zhang, et al.of RNA were subjected to hydrolysis using the Nucleoside Digestion Mix (New England BioLabs, Cat # M0649S), following manufacturer instructions. Nano-liquid chromatography was configured with a two-column system

(12) Dnmt3a2 expression during embryonic development is required for phenotypic stabilityCommunications BiologyDecember 8, 2025Minmin Liu, Guillermo Urrutia, Rachel Shereda, Galen Hostetter, Stacey L. Thomas, Gangning Liang, Peter A. Jones51140). DNA was then digested to single nucleosides using Nucleoside Digestion Mix (New England Biolabs, M0649S). Each sample, containing 200–500 ng of DNA, was digested using 5 µl of Nucleoside Digestion Mix in

(13) Carboxymethylcytosine is a natural base modification and a handle for bacteriophage DNA hypermodificationNature CommunicationsDecember 7, 2025Qiaoyu Yang, Lin Zhang, Yantao Liang, Haoyu Ma, Lifu Song, Lin Luo, Jason Tan, Yiling Hu, Kailiang Ma, Yiwei Chen, et al.dsDNA fragment was then enzymatically digested with the Nucleoside Digestion Mix (New England Biolabs, M0649S) in a 100 μL reaction mixture. The products were subjected to LC–MS analysis (Thermo Fisher Q Exactive

(14) Exploitation of phage Carin-5's own DNA polymerase to sequence its T-hypermodified genomeSci RepDecember 5, 2025Rémi Sieskind, Sophia Missoury, Frédéric Bonhomme, Pauline Nogaret, Soizick Lucas-Staat, Marc Monot, Mikael Skurnik, Anne-Claire Baudoux, Marc Delaruegene products13. Nucleoside analysis Nucleoside Digestion Mix (New England Biolabs, Ipswich, MA, cat#M0649S) was used to digest Carin-5 genomic material into nucleosides. Briefly, 50 μl reactions were prepared

(15) Protocol for RNA modification analysis by UHPLC-QqQ MSSTAR ProtocolsDecember 1, 2025Yueh-Lin Tsai, Nan Dai, Ivan R. Corrêainjection. RNA digestion to nucleosides Timing: 12 h This step uses the Nucleoside Digestion Mix (NEB, #M0649S) to achieve one-step complete digestion and dephosphorylation of the RNA samples.15.Determine RNA concentration

(16) RNA modifications, alternative splicing and circular RNA landscape in the mouse brain: inosine and beyondScientific ReportsNovember 27, 2025Erika Larrea, Maitena Tellaetxe-Abete, Yan Peng, Yujie Zhang, Shuangshuang Feng, Kaidong Lu, Liang Xue, Taisuke Nakahama, Yukio Kawahara, Magdalena J. Koziolsamples were digested into single nucleosides using Nucleoside Digestion Mix (New England BioLabs, M0649S), incubating the samples at 37 °C for 2 h. After digestion, the 2’-deoxyadenosine isotope (dA-5) (Cambridge

(17) RNA m1A methyltransferase TRMT61A promotes colorectal tumorigenesis by enhancing ONECUT2 mRNA stability and is a potential therapeutic targetCancer CommunicationsOctober 16, 2025Xiaoting Zhang, Na Qin, Fenfen Ji, Hao Su, Haiyun Shang, Hongyan Chen, Dan Huang, Qing Li, Jing Ren, Weixin Liu, et al.on ice. To digest RNA, 2.5 µL nucleoside digestion mix buffer and 0.5 µL nucleoside digestion mix (#M0649S, New England Biolabs, Ipswich, MA, USA) were added to the RNA, and the mixture was incubated at 37°C

(18) Distinct Phage‐Encoded Enzymes for Substitution of Deoxythymidine by Deoxyuridine in Phage GenomesAdvanced ScienceSeptember 26, 2025Yating Li, Jason Tan, Yanqin Tu, Jingnan Wu, Zaifang Zhang, Yifeng Wei, Xinan Jiao, Yan Zhougenomic DNA from Yersiniophage PhiR1‐37 was enzymatically digested by Nucleoside Digestion Mix (NEB M0649S) overnight at 37 °C and separated on Amicon Ultra‐0.5 mL 3 K centrifugal filters (Millipore). The flow‐through

(19) Effective Reduction in Nuclear DNA Contamination Allows Sensitive Mitochondrial DNA Methylation Determination by LC-MS/MSInternational Journal of Molecular SciencesSeptember 11, 2025Lin Liang, Luis Alfonso González Molina, Pytrick G. Jellema, Martijn van Faassen, Laura T. A. Otten, Kevin P. Mennega, Ingrid H. Hof, D. A. Janneke Dijck-Brouwer, Amalia M. Dolga, Marianne G. Rots, et al.nucleosides. DNA or RNA was digested into single nucleosides after isolation (Nucleoside Digestion Mix, cat.no. M0649S, New England Biolabs, Ipswich, UK). The digestion reaction was prepared according to the manual protocol.

(20) Uridine as a potentiator of aminoglycosides through activation of carbohydrate transportersScience AdvancesSeptember 5, 2025Manon Lang, Stéphane Renard, Imane El-Meouche, Ariane Amoura, Erick Denamur, Léo Hardy, Julia Bos, Tara Brosschot, Molly A. Ingersoll, Eric Bacqué, et al.digested into single nucleosides using the New England BioLabs Nucleoside digestion mix (catalog no. M0649S). Ten microliters of the RNA samples diluted in ultrapure water to 100 ng/μl was mixed with 1 μl of

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Nucleoside Digestion Mix

Nucleoside Digestion Mix from New England Biolabs

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Nucleoside Digestion Mix from New England Biolabs

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