Quick CIP M0525L from New England Biolabs

Product Specs
ItemQuick CIP
CompanyNew England Biolabs
Price $460.00 Inquire
Catalog NumberM0525L
Size5000 units
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Improved Cas9-targeted nanopore sequencing facilitates ultra-deep analysis of genomic variationCell Reports MethodsMay 18, 2026Nick Vereecke, Megan Behrmann, Anupama Khare, John P. Dekkervolume) was used for exhaustive dephosphorylation using the Quick Calf Intestinal Phosphatase (CIP; NEB M0525) in a 30 μL reaction volume with 10x NEB rCutSmart Buffer. The reaction was executed in a thermocycler

(2) Generation of precise and accurate engineered circRNAs using enzymatic ligationNucleic Acids ResMay 8, 2026Amrita Singh, Adela Dujsikova, Noah Mueller, Y Grace Chendephosphorylation of 1 pmol of 5′-triphosphate transcripts using 5 units of quick calf intestinal phosphatase (NEB #M0525L) at 37°C for 15 min, followed by purification and addition of monophosphate to transcripts using 10

(3) Repurposed hnRNPC binds mature mRNAs and safeguards the mitotic transcriptomeNucleic Acids ResApril 28, 2026Liat Lev-Ari, Sandra Laster, Andrea Atzmon, Daniel Blumenkrants, Daniel Benhalevy, Orna Elroy-Steinmin at 22°C), then 3′-ends of the protected RNA fragments were dephosphorylated with QuickCIP (NEB, M0525S), and ligated to a fluorescent 3′ adapter (T4 RNA ligase 2 truncated K227Q, NEB, M0351). The ligated

(4) T-cell protrusions enable fast, localised initiation of chimeric antigen receptor signallingThe EMBO JournalApril 21, 2026Carmen Rodilla-Ramirez, Giorgia Carai, Eleanor Fox, Amin Zehtabian, Helen Adam, Katja Dallio, Pia Lazki-Hagenbach, Helge Ewers, Xiaolei Su, Francesca Bottanelli,Thermo Fisher,FD1014 EcoRI-HF,NEB,R3101S HindIII-HF,NEB,R3104S Nhe-HF,NEB,R3131S NotI,NEB,R3189S Pfu polymerase,Roboklon,E1114 Quick CIP phosphatase,NEB,M0525S T4 ligase,NEB,M0202S Chemicals,Chemicals,Chemicals 2-Propanol (Isopropanol),Roth,6752.4 20x Bolt MES SDS Running Buffer

(5) The Pof1 nicotinamide mononucleotide adenylyl transferase (NMNAT) has a non-canonical role in NAD+ metabolism in the budding yeast Saccharomyces cerevisiaeJournal of Biological ChemistryApril 16, 2026Yi-Ching Lee, Chi-Chun Huang, Matilda McDaniel, Katie Huang, Lan-Hsuan Lee, Gordon Lao, Darshan Karthi, Su-Ju LinPhm8 activity was determined using the conditions described for alkaline phosphatase activity (NEB, M0525S) with modifications. Briefly, 5 μg of rPhm8 was added to 100 μl of the reaction mix (50 mM potassium

(6) Fluorescence-Guided Spatial Mapping of p21-Expressing Senescent Cells in Aged Liver at Single-Cell ResolutionBiomoleculesApril 14, 2026Jer-En Hsu, Binsheng Wang, Yongha Hwang, Weiqiu Cheng, Qingyang Zhao, Yichen Si, Ming Xu, Hyun-Min Kang, Jun Hee Leetimes initially, followed by overnight incubation of DraI (R0129, NEB, Ipswich, MA, USA) and CIAP (M0525, NEB) enzyme mixture at 37 °C. The flow cell was treated with exonuclease I (M2903, NEB) cocktail at

(7) Essentialome-Wide Multigenerational Imaging Reveals Mechanistic Origins of Cell Growth LawsbioRxivApril 12, 2026Daniel S. Eaton, Carlos Sánchez, Luis Gutiérrez-López, Jacob Q. Shenker, Youlian Goulev, Brianna R. Watson, Véronique Henriot, Ethan C. Garner, Quincey A. Justman, Jeffrey R. Moffitt, et al.Kanamycin resistance gene. This plasmid was digested with BbsI-HF (NEB, R3539S) and CIP treated (NEB, M0525S) to prevent backbone relegation at 37 °C for 1 hour. After heat inactivation at 80 °C for 20 mins, the

(8) Aimea gen. nov. defines a novel plant-associated yeast genus in Microbotryomycetes with three novel speciesbioRxivApril 10, 2026Julian A. Liber, Marco A. Coelho, Sheng-Yang HedNTPs with 0.24 U thermolabile exonuclease I (NEB cat. no. M0568) and 0.024 U QuickCIP (NEB cat. no. M0525) in 0.042x rCutSmart buffer and 0.043x NEBuffer r3.1 buffer, with 1.5 μL of PCR product in a 2.7 μL

(9) Defects in intron recycling suppress the antiviral response via a mechanism of intronic endogenous dsRNAJournal of Experimental MedicineApril 6, 2026Chaorui Duan, Luke Buerer, Cory Bowers, Allison J. Taggart, Mara H. O’Brien, Sarah Gunasekera, Chien-Ling Lin, Jing Wang, Yi Zeng, Jonathan P. Staley, et al.DNase I to remove residual DNA template, followed by treatment with calf intestinal phosphatase (Cat# M0525S; New England Biolabs) to eliminate 5′-triphosphates. Final products were purified using the Monarch

(10) The genetic architecture of an allosteric hormone receptorNature CommunicationsApril 1, 2026Maximilian R. Stammnitz, Ben Lehnerintestinal alkaline phosphatase (37 °C, 5 h) (New England Biolabs, Ipswich, USA; Catalogue No. R3131, R3104, M0525). The second, nucleotide-sequence recoded copy of wild-type PYL1 was then amplified from an IDT gBlock

(11) Efficient genetic perturbation of murine sensory neurons in vivo using CRISPR/Cas9The Journal of PainApril 1, 2026Guadalupe García, Jacob B. Shapiro, Zachary T. Campbell,New England Biolabs,E2040L "DNase I, RNase-free",Thermo Scientific,EN0521 Quick CIP,New England Biolabs,M0525S Bleach,Clorox, RNase Away,Molecular Bioproducts,7003 DEPC,Sigma-Aldrich,40718-100ML Ethanol,Sigma-Aldrich,1.08543.0250 Agarose,Research

(12) 5-Azacytidine incorporation into mRNAs disrupts translation and induces ribosome collisionsbioRxivMarch 31, 2026Alexis B Roberson, James Marks, Ruby Pitts, Bavavarshini Tamilselvam, Brian Grieb, William P Tansey, Sezen MeydanBsmB1-v2 restriction enzyme (NEB, R0739) and Buffer r3.1 (NEB, B6003), dephosphorylated by using CIP (NEB, M0525), and then gel purified. The digested vector was then ligated with 1:200 diluted oligo duplex by using

(13) Pathogenic CGG expansions in oculopharyngodistal myopathy exhibit distinct characteristics of each causative gene on the flanking sequences as well as methylation statusGenome MedMarch 27, 2026Nobuyuki Eura, Satoru Noguchi, Megumu Ogawa, Kyuto Sonehara, Ai Yamanaka, Takashi Kurashige, Shinichiro Hayashi, Yukinori Okada, Kazuma Sugie, Ichizo Nishino1: Table S1). Genomic DNA (2 μg) was dephosphorylated with Quick Calf Intestinal Phosphatase (NEB, #M0525S) at 37 °C for 10 min, followed by inactivation at 80 °C for 2 min. The dephosphorylated genomic DNA

(14) CBP-IDRs regulate acetylation and gene expressionCell RepMarch 24, 2026Katie L. Gelder, Nicola A. Carruthers, Grace Gilbert, Laura J. Harrison, Brychan V. Evans, Thomas I. Evans, Sophie S. Ball, Mark Dunning, Timothy D. Craggs, Alison E. Twelvetrees, et al.(PNK),NEB,EK0031 NruI-HF,NEB,R3192S PmlI,NEB,R0532S Quick Calf Intestinal Phosphatase (CIP),NEB,M0525S BamHI-HF,NEB,R3136S MluI-HF,NEB,R3198S BssHII,NEB,R0199S BbsI-HF,NEB,R3539S QuickExtract solution,Lucigen,QE0905T T7

(15) MASTR-seq enables multiplexed analysis of short tandem repeats with sequencingCell Rep MethodsMarch 23, 2026Chuanbin Su, Han-Seul Ryu, Keerthivasan Raanin Chandradoss, Thomas Malachowski, Ravi Boya, Linda Zhou, Hoa Emma Nguyen, Esteban O. Mazzoni, Kristen J. Brennand, Jennifer E. Phillips-Creminsdephosphorylation For each reaction, we used 5 μg DNA and dephosphorylated it with 3 μL QuickCIP (NEB# M0525S) in 1x rCutSmart Buffer. We commonly setup 2–3 replicates for each sample. Employ a thermal cycler to

(16) The E3 ubiquitin ligase mechanism specifying targeted microRNA degradationNatureMarch 18, 2026Jakob Farnung, Elena Slobodyanyuk, Peter Y. Wang, Lianne W. Blodgett, Daniel H. Lin, Susanne von Gronau, Brenda A. Schulman, David P. Bartelurea-polyacrylamide gel. In vitro transcription products were first dephosphorylated using Quick CIP (NEB, M0525) at 37 °C for 15 min, followed by heat-inactivation at 80 °C for 3 min. Dephosphorylated RNAs were then

(17) Multiplexed optical barcoding and sequencing for spatial omicsScientific ReportsMarch 18, 2026Aditya Venkatramani, Didar Ciftci, Khanh Pham, Limor Cohen, Leonardo Sepulveda, Christopher Li, Xiaowei Zhuangbuffer composed of 1x rCutsmart buffer (NEB, B6004S), 0.1 % TX-100, 250 U/ml quick CIP enzyme (NEB, M0525S) for 20 min at 37 °C to remove any free 5’ phosphate groups that may have formed due to stray light

(18) RSRC2 is a novel RNA-binding protein that safeguards mitotic fidelity by interacting with the lncRNA C1QTNF1-AS1Nucleic Acids ResMarch 17, 2026Parnia Babaei, Alice O Coomer, Kaliya Georgieva, Giulia Guiducci, Elisa Vitiello, Martin Dodel, Eleni Maniati, Hanya Elsayed Eid, Anisha Thind, Sneha Krishnamurthy, et al.restriction endonuclease (R0519S, NEB) at 65°C for 1 h followed by its dephosphorylation using the Quick CIP (M0525S, NEB) at 37°C for 10 min. The linearized vector was run on a 1% agarose gel, and the products were extracted

(19) ZAP targets aberrant mRNA transcripts encoding proteins with defective signal peptides for degradationThe EMBO JournalMarch 12, 2026Akruti Shah, Aaztli R Coria, Britnie Santiago Membréno, Emilien Orgebin, Jennifer T Miller, Wilfried Guiblet, Colin Chih-Chien Wuinhibitor cocktail,Sigma-Aldrich,11836170001 Puromycin,Sigma-Aldrich,P7255 Quick CIP,New England Biolabs,M0525L RNase A,New England Biolabs,T3018 RNase I,Thermo Fischer Scientific,AM2294 SUPERaseIn,Thermo Fischer

(20) La protein binding to telomerase RNA supports an evolutionary relationship between plant and ciliate telomerase pathwaysNucleic Acids ResMarch 10, 2026Leon Jenner, Dzmitry Pruchkouski, Barbora Štefanovie, Olga Nováková, Monika Kubíčková, Petr Fajkus, Marie Brázdová, Jan Paleček, Eva Sýkorovásynthesized RNA was dephosphorylated using incubation with Quick Calf Intestinal Phosphatase (Quick CIP #M0525, New England BioLabs) for 1 h at 37°C, before purification using AMPure XP magnetic beads (Beckman Coulter,

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Quick CIP

Quick CIP from New England Biolabs

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Quick CIP from New England Biolabs

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