Apyrase M0398L from New England Biolabs

Product Specs
ItemApyrase
CompanyNew England Biolabs
Price $320.00 Inquire
Catalog NumberM0398L
Size50 units
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Purification of the Active-State G Protein-Coupled Receptor ADGRL4 for Cryo-Electron Microscopy Using a Modular Tag System and a Tethered mini-G_qBIO-PROTOCOLMarch 5, 2026David M. Favara, Christopher G. Tate(Sigma-Aldrich, catalog number: 75259-1G) 14. Apyrase (500 units/mL), 50 units (NEB, catalog number: M0398L); store at -20 °C 15. Phenylmethylsulfonyl fluoride (PMSF) protease inhibitor, 5 g (Thermo Scientific,

(2) Molecular insights into ligand recognition and receptor activation of GPR15iScienceDecember 19, 2025Shutian Chen, Xuteng Han, Yuxia Zhang, Limin Ma, Cuiying Yi, Xiaojing Chu, Qiuxiang Tan, Shuo Han, Qiang Zhao, Beili WuP515 EDTA-free protease inhibitor cocktail,"TargetMol, USA",Cat# C0001 Apyrase,New England Biolabs,Cat# M0398L ESF 921 Insect Cell Culture Medium,Expression Systems,Cat# 96-001-01 Fetal Bovine Serum (FBS),GEMINI,Cat#

(3) Structural insights into small-molecule agonist recognition and activation of complement receptor C3aRThe EMBO JournalApril 7, 2025Jinuk Kim, Saebom Ko, Chulwon Choi, Jungnam Bae, Hyeonsung Byeon, Chaok Seok, Hee-Jung ChoiPhenylmethylsulfonyl fluoride,Sigma-Aldrich,Cat#11359061001 Tris(2-carboxyethyl)phosphine hydrochloride,Goldbio,Cat#TCEP1 Apyrase,NEB,Cat#M0398L Dulbecco’s Modified Eagle Medium (DMEM),Cytiva,Cat#SH30243.01 Fetal Bovine Serum,GW vitek,Cat#US-FBS-500 Antibiotic-Antimycotic,Gibco,Cat#15240–062 Lipofectamine

(4) Structural insights into the selective recognition of RF-amide peptides by neuropeptide FF receptor 2EMBO ReportsMarch 24, 2025Jeesoo Kim, Sooyoung Hong, Hajin Lee, Hyun Sik Lee, Chaehee Park, Jinuk Kim, Wonpil Im, Hee-Jung ChoiPhenylmethylsulfonyl fluoride,Sigma-Aldrich,Cat#11359061001 Tris(2-carboxyethyl)phosphine hydrochloride,Goldbio,Cat#TCEP1 Apyrase,NEB,Cat#M0398L Dulbecco’s Modified Eagle Medium (DMEM),Cytiva,Cat#SH30243.01 Fetal Bovine Serum,GWvitek,Cat#US-FBS-500 Antibiotic-Antimycotic,Gibco,Cat#15240-062 Lipofectamine

(5) A subcellular map of translational machinery composition and regulation at the single-molecule levelScienceMarch 7, 2025Zijian Zhang, Adele Xu, Yunhao Bai, Yuxiang Chen, Kitra Cates, Craig Kerr, Abel Bermudez, Teodorus Theo Susanto, Kelsie Wysong, Fernando J. García García Marqués, et al.(ThermoFisher AM2696), 0.014 U/μL Turbo DNAse (ThermoFisher AM2239), 1 U/mL apyrase (New England Biolabs M0398L) in Ultrapure distilled water (ThermoFisher 10977-015). Lysis buffer for Western blot or mass spectrometry: 20

(6) DDM1-mediated R-loop resolution and H2A.Z exclusion facilitates heterochromatin formation in ArabidopsisSci AdvAugust 11, 2023Jincong Zhou, Xue Lei, Sarfraz Shafiq, Weifeng Zhang, Qin Li, Kuan Li, Jiafu Zhu, Zhicheng Dong, Xin-Jian He, Qianwen Sunpurified by phenol:chloroform extraction. R-loop–containing products were treated by Apyrase (NEB, M0398L) to remove excess ribonucleoside adenosine triphosphate (rATP) and then were purified by phenol:chloroform.

(7) TRIM21-dependent target protein ubiquitination mediates cell-free Trim-AwayCell reportsFebruary 28, 2023Mevissen TET, Prasad AV, Walter JC.E2–664 "Recombinant Human UbcH5b/UBE2D2 Protein, CF",R&D Systems,Cat# E2–622 Apyrase,New England Biolabs,Cat# M0398L Critical commercial assays,, Reductive Alkylation Kit,Hampton Research,Cat# HR2–434 TnT® SP6 High-Yield Wheat Germ Protein Expression System

(8) From Guide to Guard – Activation Mechanism of the Stress-Sensing Chaperone Get3Molecular CellSeptember 1, 2022Kathrin Ulrich, Ákos Farkas, Olivia Chan, Olivia Katamanin, Blanche Schwappach, Ursula JakobBioscience,Cat# NU-202L 4-(2-pyridylazo) resorcinol (PAR),Sigma-Aldrich,Cat#323209 Apyrase,NEB,Cat#M0398L Citrate synthase from porcine heart,Sigma-Aldrich,Cat#C3260 DNase,Thermo Fisher,Cat#EN0521 EDTA-free

(9) A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylationeLifeJanuary 31, 2022Andrew Loffer, Jasleen Singh, Akihito Fukudome, Vibhor Mishra, Feng Wang, Craig S PikaardCi/mmol",PerkinElmer,Cat #BLU002Z250UC,Used for end-labeling RNA "Chemical compound, drug",Apyrase,NEB,Cat # M0398L,Hydrolyzes ATP; used in Figure 5 "Chemical compound, drug","SYBR Gold Nucleic Acid Gel Stain (10,000×)",Invitrogen,Cat

(10) mTORC1 promotes cell growth via m⁶A-dependent mRNA degradationMolecular cellMay 20, 2021Cho S, Lee G, Pickering BF, Jang C, Park JH, He L, Mathur L, Kim SS, Jung S, Tang HW, et al.,Thermo Fisher Scientific,Cat#AM8740 T4 Polynucleotide Kinase,New England Biolabs,Cat#M0201S Apyrase,New England Biolabs,Cat#M0398L TRIzol,Invitrogen,Cat#15596018 Protein A/G magnetic beads,Thermo Fisher Scientific,Cat#88802 Proteinase K

(11) Cryo-EM Structure of the Prostaglandin E Receptor EP4 Coupled to G ProteinStructureMarch 1, 2021Shingo Nojima et al.GM ONO-AE3-208 Cayman Cat# 14522 PGE2 Cayman Cat# 14010 PF-04418948 Funakoshi Cat# 15016 Apyrase NEB Cat# M0398L D-luciferin FujiFilm Wako Pure Chemical Corporation Cat# 127-03941 Promega Cat# E2301 PGE2-EP4-Gsb1g2-Nb35 complex

(12) Structural insights into the human D1 and D2 dopamine receptor signaling complexesCellFebruary 10, 2021Youwen Zhuang et al.C112 "Protease Inhibitor Cocktail, EDTA-free",Bimake,Cat# B14003 Apyrase,New England Biolabs,Cat# M0398L Nickel Sepharose resin,GE healthcare,Cat#17526801 Anti-Flag resin,Smart-Lifesciences,Cat# C20042002 FLAG

(13) The selectivity filter of the mitochondrial protein import machineryBMC BiologyDecember 1, 2020Sebastian Kreimendahl, Jan Schwichtenberg, Kathrin Günnewig, Lukas Brandherm, Joachim Rassowboth the reticulocyte lysate and the mitochondria were preincubated in parallel with apyrase (NEB, M0398L) at a final concentration of 25 U/ml for 20 min at 25 °C. The procedure followed the protocol of [31].

(14) Structures of Gα Proteins in Complex with Their Chaperone Reveal Quality Control MechanismsCell ReportsMarch 2, 2020Alpay Burak Seven, Daniel Hilger, Makaía M. Papasergi-Scott, Li Zhang, Qianhui Qu, Brian K. Kobilka, Gregory G. Tall, Georgios Skiniotis62970 Chelating Sepharose Fast Flow,GE Healthcare,Cat# 17057502 DTT,Goldbio,Cat# DTT Apyrase,NEB,Cat# M0398L Glutathione Resin,Genscript,Cat# L00206 Casein Kinase II (CKII),NEB,Cat# P6010L Guanosine 5’-O-(3-thiotriphosphate)

(15) The gene silencing protein MORC-1 topologically entraps DNA and forms multimeric assemblies to cause DNA compaction.Molecular CellAugust 22, 2019HyeongJun Kim et al.pH 7.5, 500 mM NaCl, 30 mM imidazole, 5 mM DTT, then treated with at least 2 units of apyrase (NEB M0398L) prior to elution except for the experiment shown in Supplementary Fig. 2. Protein was eluted from the

(16) A G_s-coupled purinergic receptor boosts Ca²⁺ influx and vascular contractility during diabetic hyperglycemiaeLifeMarch 1, 2019Prada MP, Syed AU, Buonarati OR, Reddy GR, Nystoriak MA, Ghosh D, Simó S, Sato D, Sasse KC, Ward SM, et al.,Santa Cruz Biotechnology,sc-361274, "Chemical compound, drug",NF546,Tocris,3892, "Chemical compound, drug",apyrase,New England Biolabs,M0398L, "Chemical compound, drug",nifedipine,Sigma-Aldrich,N7634, "Chemical compound, drug",Bay K-8644,Sigma-Aldrich,71145-03-4, "Chemical

(17) A Linear Diubiquitin-Based Probe for Efficient and Selective Detection of the Deubiquitinating Enzyme OTULINCell chemical biologyOctober 19, 2017Weber A, Elliott PR, Pinto-Fernandez A, Bonham S, Kessler BM, Komander D, El Oualid F, Krappmann D.745400.100 "Roti-Load 1, 4x conc.",Carl Roth,Cat#K929.1 E.coli recombinant Apyrase,New England Biolabs,Cat#M0398L Sequence Grade Modified Trypsin,Promega,Cat#V5111 ,, Critical Commercial Assays,Critical Commercial

(18) Genomic Nucleosome Organization Reconstituted with Pure ProteinsCellOctober 20, 2016Nils Krietenstein, Megha Wal, Shinya Watanabe, Bongsoo Park, Craig L. Peterson, B. Franklin Pugh, Philipp Korberwith glycine (125 mM final concentration) at 30 °C for 5 min and treatment with 200 mU apyrase (NEB, M0398L) for 30 min. For MNase-seq, reconstitution reactions were stopped only by apyrase treatment at 30 °C

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Apyrase

Apyrase from New England Biolabs

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New England Biolabs

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Apyrase from New England Biolabs

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