Apyrase M0398S from New England Biolabs

Product Specs
ItemApyrase
CompanyNew England Biolabs
Price $79.00 Inquire
Catalog NumberM0398S
Size10 units
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Molecular fingerprints of a convergent mechanism orchestrating diverse ligand recognition and species-specific pharmacology at the complement anaphylatoxin receptorsbioRxivMay 30, 2025Sudha Mishra, Manish K. Yadav, Annu Dalal, Manisankar Ganguly, Ravi Yadav, Kazuhiro Sawada, Divyanshu Tiwari, Nabarun Roy, Nilanjana Banerjee, Jenny N. Fung, et al.Gαo, Gβ1γ2, and ScFv16 at room temperature for 2 h in the presence of 25 mU/mL apyrase (NEB, Cat. no: M0398S) and either 1 μM of mC3a, mC5a, hC5a-d-Arg, mC5a-d-Arg or 10 μM hTLQP21, mTLQP21, EP67, EP54, SB290157,

(2) ZNF574 is a quality control factor for defective ribosome biogenesis intermediatesMolecular CellMay 15, 2025Jared F. Akers, Michael LaScola, Adrian Bothe, Hanna Suh, Carmen Jung, Zachary D. Stolp, Tanushree Ghosh, Liewei L. Yan, Yuming Wang, Michelle Macurak, et al.DTT, 24 U/ml Turbo DNase (Ambion, AM2239), 20 U/mL SUPERaseIn (Ambion, AM2696), 1U/ml Apyrase (NEB M0398S) and then loaded onto gradients. Gradients were centrifuged for 1 hour 45 minutes at 40,000 rpm, 4°C

(3) Structural visualization of small molecule recognition by CXCR3 uncovers dual-agonism in the CXCR3-CXCR7 systemNat CommunMarch 28, 2025Shirsha Saha, Fumiya K. Sano, Saloni Sharma, Manisankar Ganguly, Annu Dalal, Sudha Mishra, Divyanshu Tiwari, Hiroaki Akasaka, Takaaki A. Kobayashi, Nabarun Roy, et al.excess of miniGαo, Gβ1γ2, and scFv16, in the presence of 5 mM CaCl2 and 25mU/mL apyrase (NEB, Cat. no: M0398S), for 2 h at room temperature. The mixture was then concentrated using a 100 MWCO concentrator (Cytiva,

(4) Molecular basis of promiscuous chemokine binding and structural mimicry at the C-X-C chemokine receptor, CXCR2Molecular CellMarch 6, 2025Shirsha Saha, Fumiya K. Sano, Saloni Sharma, Manisankar Ganguly, Sudha Mishra, Annu Dalal, Hiroaki Akasaka, Takaaki A. Kobayashi, Nashrah Zaidi, Divyanshu Tiwari, et al.1.2-fold molar excess of mGαoA, Gβ1γ2, and scFv16, in the presence of 25mU/mL apyrase (NEB, Cat. no: M0398S) and 5mM CaCl2, for 2h at room temperature. The mixture was concentrated using 100 MWCO concentrator

(5) Structural basis of THC analog activity at the Cannabinoid 1 receptorNat CommunJanuary 8, 2025Thor S. Thorsen, Yashraj Kulkarni, David A. Sykes, Andreas Bøggild, Taner Drace, Pattarin Hompluem, Christos Iliopoulos-Tsoutsouvas, Spyros P. Nikas, Henrik Daver, Alexandros Makriyannis, et al.mM MgCl2, 2 μM HU-210 (Tocris Cat. No. 0966), ~10 μg/ml ScFv16, 0.025 units/ml Apyrase (NEB Cat. No. M0398S) and Roche EDTA free protease inhibitors (Sigma Cat. No. REF05056489001). The lysed cells were harvested

(6) Native mass spectrometry prescreening of G protein-coupled receptor complexes for cryo-EM structure determinationStructureDecember 1, 2024Donggyun Kim, Weijing Liu, Rosa Viner, Vadim Cherezov,Millipore Sigma,Cat#C3416 "HRV 3C protease, His-tagged",Genscript,Cat#Z03092 Apyrase,New England Biolabs,Cat#M0398S HisPur™ Ni-NTA resin,Thermo Scientific,Cat#88221 AminoLink™ coupling resin,Thermo Scientific,Cat#20381 "n-Dodecyl-N,N-dimethylamine-N-oxide

(7) Structural basis of ligand recognition and activation of the histamine receptor familyNature CommunicationsSeptember 27, 2024Xuan Zhang, Guibing Liu, Ya-Ni Zhong, Ru Zhang, Chuan-Cheng Yang, Canyang Niu, Xuanyu Pu, Jingjing Sun, Tianyao Zhang, Lejin Yang, et al.buffer containing 20 mM HEPES pH 7.5, 50 mM NaCl, 10 mM MgCl2, 5 mM CaCl2, 25 mU/ml Apyrase (NEB, Cat# M0398S), 1–10 μM agonist, 2.5 μg/ml leupeptin (Sigma-Aldrich, Cat# L2884), 300 μg/ml benzamidine (Sigma-Aldrich,

(8) Structure-guided engineering of biased-agonism in the human niacin receptor via single amino acid substitutionNat CommunMarch 2, 2024Manish K. Yadav, Parishmita Sarma, Jagannath Maharana, Manisankar Ganguly, Sudha Mishra, Nashrah Zaidi, Annu Dalal, Vinay Singh, Sayantan Saha, Gargi Mahajan, et al.a 1.2 molar excess of miniGo, Gβy, and ScFv16 in the presence of 25 mµ ml-1 apyrase (NEB, Cat. no. M0398S) and 1 µM of individual ligand, and complexing was allowed for two hours at room temperature. The receptor

(9) The N1 domain of the peroxisomal AAA-ATPase Pex6 is required for Pex15 binding and proper assembly with Pex1Journal of Biological ChemistryJanuary 1, 2024Bashir A. Ali, Ryan M. Judy, Saikat Chowdhury, Nicole K. Jacobsen, Dominic T. Castanzo, Kaili L. Carr, Chris D. Richardson, Gabriel C. Lander, Andreas Martin, Brooke M. Gardnerwas performed after a 10 min 30 °C incubation in ATP regeneration mix with and without apyrase (NEB#M0398S, 500 units/ml, 62.5 units/ml final concentration). AlphaFold2-multimer predictions AlphaFold2-multimer

(10) Structural basis of selective cannabinoid CB(2) receptor activationNature communicationsMarch 15, 2023Li X, Chang H, Bouma J, de Paus LV, Mukhopadhyay P, Paloczi J, Mustafa M, van der Horst C, Kumar SS, Wu L, et al.of 25 μM agonist (LEI-102, APD371, HU308, and CP55,940, respectively) and 2 units of apyrase (NEB, #M0398S) in the presence 500 µg scFv16. The lysate was incubated for overnight at 4 °C and discard the supernatant

(11) Condensin pinches a short negatively supercoiled DNA loop during each round of ATP usageThe EMBO JournalFebruary 1, 2023Belén Martínez‐García, Sílvia Dyson, Joana Segura, Alba Ayats, Erin E Cutts, Pilar Gutierrez‐Escribano, Luís Aragón, Joaquim RocaI (TopA; NEB #M0301S); Nuclease P1 (NEB #M0660S); Alkaline Phosphatase (NEB #M0290); Apyrase (NEB #M0398S); Endonuclease BspQI (NEB #R06445); T4 DNA ligase (NEB #M0202T). To produce a stock of relaxed DNA,

(12) Measurements of the Force-Dependent Detachment Rates of Cytoplasmic Dynein from MicrotubulesMethods in Molecular BiologyJanuary 19, 2023Xinglei Liu, Lu Rao, Arne Gennerichcatalyzes the hydrolysis of ATP to AMP releasing inorganic phosphate, 500 units/mL (New England BioLabs, #M0398S). C-Trap™ Optical Tweezer (Lumicks). 3 Methods 3.1 Yeast Protein Expression and Cell Harvest This

(13) Global insights into the fine tuning of human A2AAR conformational dynamics in a ternary complex with an engineered G protein viewed by NMRCell ReportsDecember 20, 2022Guillaume Ferré, Kara Anazia, Larissa O. Silva, Naveen Thakur, Arka P. Ray, Matthew T. Eddy5’-N-ethylcarboxamidoadenosine,Tocris,Cat#1691 Guanosine 5’-diphosphate (GDP),Sigma-Aldrich,Cat#G7127 Apyrase,NEB,Cat#M0398S ,, Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models:

(14) Structural basis of the ligand binding and signaling mechanism of melatonin receptorsNature CommunicationsDecember 1, 2022Qinggong Wang, Qiuyuan Lu, Qiong Guo, Maikun Teng, Qingguo Gong, Xu Li, Yang Du, Zheng Liu, Yuyong TaoCaCl2, 1 mM MnCl2, 100 μU ml−1 lambda phosphatase (NEB Cat# P0753S), and 25 μU ml−1 apyrase (NEB Cat# M0398S), and then incubated at 4 °C for 90 min. The supernatant was isolated by centrifugation at 28,000 ×

(15) Cryo‐EM structure of G‐protein‐coupled receptor GPR17 in complex with inhibitory G proteinMedComm (2020)December 1, 2022Fang Ye et al.(Sigma–Aldrich Cat# 12072), 0.2 μg/ml leupeptin (Sigma‐Aldrich Cat# L5793), 25 μU/ml apyrase (NEB Cat# M0398S), and 100 μU/ml lambda protein phosphatase (NEB Cat# P0753S). Then, 1% DDM (n‐Dodecyl‐β‐D‐Maltoside,

(16) Structural and functional asymmetry of RING trimerization controls priming and extension events in TRIM5Î± autoubiquitylationNat CommunNovember 19, 2022Frank Herkules, Corey H. Yu, Alexander B. Taylor, Vi Dougherty, Susan T. Weintraub, Dmitri N. IvanovThe reactions were then quenched by depleting ATP with 2 U/mL of apyrase (New England BioLabs Cat# M0398S) for 5 min at room temperature. The reactions were then diluted and dispensed into 384-well fluorescence

(17) Structures of oxysterol sensor EBI2/GPR183, a key regulator of the immune responseStructureJuly 1, 2022Hongwen Chen, Weijiao Huang, Xiaochun LiExpress,Cat#HY-101192 GDP,Sigma-Aldrich,Cat#G7127 TCEP,Thermo Fisher Scientific,Cat#77720 Apyrase,New England Biolabs,Cat#M0398S Sf-900 III SFM,Gibco,Cat#12658027 FreeStyle 293 Expression Medium,Gibco,Cat#12338018 RPMI 1640 medium,Corning,Cat#10–040-CV "Penicillin-Streptomycin

(18) Purification and cryo-EM structure determination of VCP/p97 dodecamers from mammalian and bacterial cellsSTAR ProtocolsJune 17, 2022Guimei Yu, Yunpeng Bai, Zhong-Yin Zhangagarose,QIAGEN,Cat#30230 PfuTurbo DNA Polymerase,Agilent,Cat#600252 Apyrase,New England Biolabs,Cat#M0398S HindIII,New England Biolabs,Cat#R0104L BamHI,New England Biolabs,Cat#R0136L NdeI,New England Biolabs,Cat#R0111L T4 DNA ligase

(19) Cryo-electron microscopy structures of VCP/p97 reveal a new mechanism of oligomerization regulationiScienceNovember 19, 2021Guimei Yu, Yunpeng Bai, Kunpeng Li, Ovini Amarasinghe, Wen Jiang, Zhong-Yin Zhang,Sigma Aldrich,A1388 Adenosine 5′-triphosphate (ATP) disodium salt hydrate,Sigma Aldrich,FLAAS-10VL Apyrase,NEB,Cat # M0398S DMEM with glucose,Corning Cellgro,10-013-CV Penicillin-Stremptomycin Solution (100x),Corning,MT30002CI Fetal

(20) Phagocyte Chemoattraction Is Induced through the Mcp-1-Ccr2 Axis during EfferocytosisCellsNovember 10, 2021Lee SA, Kim D, Min C, Moon B, Lee J, Moon H, Yang S, Lee CS, Lee G, Park D.(C8273-1MG, Sigma-Aldrich, Burlington, MA, USA), bafilomycin A1 (ab120497, Abcam, Waltham, MA, USA), apyrase (M0398S, NEB, Ipswich, MA, USA), PS beads (P-BOPS, Echelon Biosciences, Salt Lake City, UT, USA), TAMRA-SE (C1171,

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Apyrase

Apyrase from New England Biolabs

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New England Biolabs

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Apyrase from New England Biolabs

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