SplintR Ligase M0375S from New England Biolabs

Product Specs
ItemSplintR Ligase
CompanyNew England Biolabs
Price $106.00 Inquire
Catalog NumberM0375S
Size1250 units
ApplicationsFunctional Genomics
TypeEnzymes
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Programmable ZBTB7A RNA N6-methyladenosine site-specific demethylation suppresses colorectal cancer liver metastasisMol CancerAugust 14, 2026Xuena Chen, Shuo Zhao, Bochen Liu, Yujia Fan, Huanlu Qiu, Lingtong Ren, Xinrui Zhang, Shurui Yan, Yachun An, Shirong Li, et al.3 μl enzyme mixtures containing 0.01 U Bst 2.0 DNA polymerase (M0537S, NEB), 0.5 U SplintR ligase (M0375S, NEB) and 10 nmol ATP (P0756S, NEB). The final 20 μl reaction mixtures were incubated at 40 °C for 20

(2) Neuronal YTHDF2 suppresses innate immune activation in Aβ pathology by promoting m6A-dependent decay of cytosolic mitochondrial mRNAsSci AdvJune 19, 2026Wenqi Pan, Lin Yang, Yao Zhang, Yan Chen, Yuesi Xu, Yifan Li, Yujie Fu, Chunhui Ma, Chunying Liu, Qing Li, et al.mixture was combined with 0.01 U of Bst 2.0 DNA Polymerase (NEB, M0537S), 0.5 U of SplintR Ligase (NEB, M0375S), and 10 nM adenosine 5′-triphosphate (ATP; NEB, P0756S) to a final volume of 20 μl. The final reaction

(3) Quantitative RNA pseudouridine landscape reveals dynamic modification patterns and evolutionary conservation across bacterial speciesElifeJune 4, 2026Letong Xu, Shenghai Shen, Yizhou Zhang, Zhihao Guo, Beifang Lu, Jiadai Huang, Runsheng Li, Yitong Shen, Li-Sheng Zhang, Xin Dengmin, 70°C for 1 min, 60°C for 1 min, 50°C for 1 min, and 40°C for 6 min). 2 µl of SplintR ligase (NEB #M0375S) was then added, and ligation was carried out at 40°C for 60 min, followed by denaturation at 95°C for

(4) Antisense oligonucleotides reverse SPTLC1-related hereditary sensory neuropathy in a mouse modelBrainJune 3, 2026Jinhong Meng, Shunyi Ma, Museer A Lone, Hou Wang Lam, Qiang Zhang, Shuzhi Cheng, Shona Mackie, Emma Graham, Hanna Kedzior, Charalambos Demetriou, et al.tissue lysate was hybridized and ligated with probes using a SplintR ligase kit (New England Biolabs, M0375S), following manufacturer’s instructions. After ligation, qRT-PCR was performed using a PrimeTime™ Gene Expression Master Mix

(5) Simultaneous brain-wide single-cell recording resolves spatiotemporal memory architecturebioRxivMay 22, 2026Dongqing Shi, Yongjie Hou, Yixiao Yan, Tian-hao Zhang, William C. Joesten, Peng Liu, Yixuan Wang, Mehul Gautam, Jormay Zheng, Lirong Zheng, et al.temperature in a 300-μL reaction containing 6 μL SplintR ligase (5 U/μL; final concentration, 0.1 U/μL; NEB M0375S), 30 μL 10× ligase buffer, 3 μL BSA (20 mg/mL; final concentration, 0.2 mg/mL; NEB B9200S), 1 μL RNase

(6) Epitranscriptomic regulation of mRNA stability in pivotal transcription factors modulates the second cell fate decisionNature CommunicationsApril 28, 2026Weide Xiao, Li Tang, Mingli Ma, Kuisheng Liu, Jiaxu Lu, Wenqing Ding, Zhile Bai, Xuelian Liu, Xiaochen Kou, Yanhong Zhao, et al.Subsequently, the mixture was incubated with 3 μL of 0.01 U Bst 2.0 DNA polymerase, 0.5 U SplintR ligase (NEB, M0375S), and 10 nM ATP at 40 °C for 20 min, followed by denaturation at 80 °C for 20 min. A 20 μL qPCR reaction

(7) A platform for multimodal in vivo pooled genetic screens reveals regulators of liver functionbioRxivApril 13, 2026Reuben A. Saunders, William E. Allen, Xingjie Pan, Jaspreet Sandhu, Jiaqi Lu, Thomas K. Lau, Karina Smolyar, Zuri A. Sullivan, Catherine Dulac, Jonathan S. Weissman, et al.Ammonium persulfate,Sigma,Cat# A3678 PEG35000,Sigma,Cat# 94646 Tween 20,Sigma,Cat# P9416 SplintR Ligase,NEB,Cat# M0375S NxGen phi29 DNA Polymerase,Lucigen,Cat# 30221-3 dNTP Mix,NEB,Cat# N0447S Aminoallyl-dUTP,ThermoFisher,Cat#

(8) StALKBH10B ‐Mediated RNA m 6 A Modification Inhibits Potato Salt Tolerance by Targeting Flavonoids and ABA Signalling PathwaysPlant Biotechnology JournalMarch 19, 2026Xuanming Dong, Jianyu Ma, Chenxin Sui, Yan Li, Vadim Khassanov, Hongju Jian, Dianqiu Lvsupplemented with 0.01 unit Bst 2.0 DNA polymerase (NEB, USA, M0537S), 0.5 unit SplintR ligase (NEB, USA, M0375S), and 10 nmol ATP to a final volume of 20 μL, and then incubated at 40°C for 20 min, followed by at

(9) Directed evolution of an m6A eraser for site-selective epitranscriptome editingResearch SquareFebruary 23, 2026Chuan-Hui Zhou, Chuan-Hui WangAlkaline Phosphatase (Thermo Fisher Scientific, EF0654), BST DNA polymerase (NEB, M0537S), SplintR ligase (NEB, M0375S), and DNase I (Fisher Scientific, FEREN0521) were obtained from the indicated suppliers. Antibodies

(10) The dynamic landscape and conserved regulation of the m6Am and m6A methylomes during the human and mouse oocyte-to-embryo transitionNucleic Acids ResFebruary 10, 2026Ruibao Su, Zongchang Du, Sen Li, Hanyan Liu, Di Gao, Shaoquan Zhan, Chao Li, Tie-Gang Meng, Lei-Ning Chen, Li-Hua Fan, et al.containing Bst 2.0 DNA polymerase (New England BioLabs, M0537S), SplintR ligase (New England BioLabs, M0375S), and ATP (New England BioLabs, P0756S) was added. The reaction was incubated at 40°C for 20 min, followed

(11) Heteroduplex oligonucleotide technology boosts gene knockdown in cardiac and skeletal musclesNucleic Acids ResearchJanuary 28, 2026Sakino Matsuda-Kawabata, Tetsuya Nagata, Mitsugu Yanagidaira, Kensuke Ihara, Masaki Ohyagi, Hidetoshi Kaburagi, Kie Yoshida-Tanaka, Asuka Sasaki, Aya Abe, Satoe Ebihara, et al.mixed with 2 µl of 10 × SplintR Ligase Reaction Buffer (New England Biolabs, Ipswich, MA, USA; Cat. M0375S), 2 µl each of probe A (100 nM) and probe B (100 nM), and nuclease-free water to a final volume of 12

(12) Potyviruses recruit host eIF4A3 to block m6A-mediated RNA decay by steric hindrance of viral RNA methylation in plantsNucleic Acids ResearchJanuary 8, 2026Dezhi Peng, Laihua Dong, Pei Wang, Lianyi Zang, Jinhao Xie, Hao Wang, Xiangdong Li, Zaifeng Fan, Tao Zhou, Kaitong Du1 min, followed by 50°C for 1 min, and 40°C for 6 min. Subsequently, 0.5 U of SplintR Ligase (NEB, M0375S), 0.01 U of Bst 2.0 DNA Polymerase (NEB, M0537S), and 10 nmol ATP are added to the mixture with a final

(13) Pseudouridylation of 7SK by PUS7 regulates Pol II transcription elongationNature communicationsOctober 30, 2025Zhao Y, Sun HL, Li W, Ye C, Dou X, Peng Y, Wu T, Wang P, Ju CW, Liu S, et al.for 20 min. Another 10 μL of a mixture containing 15% PEG8000, 6 mM ATP, 2.5 U SplintR Ligase (NEB, M0375S), and 1X CutSmart buffer was added, and the reaction was incubated at 25 °C for 20 min. The reaction

(14) Perturb-Multimodal: A platform for pooled genetic screens with imaging and sequencing in intact mammalian tissueCellAugust 21, 2025Reuben A. Saunders, William E. Allen, Xingjie Pan, Jaspreet Sandhu, Jiaqi Lu, Thomas K. Lau, Karina Smolyar, Zuri A. Sullivan, Catherine Dulac, Jonathan S. Weissman, et al.Ammonium persulfate,Sigma,Cat# A3678 PEG35000,Sigma,Cat# 94646 Tween 20,Sigma,Cat# P9416 SplintR Ligase,NEB,Cat# M0375S NxGen phi29 DNA Polymerase,Lucigen,Cat# 30221–3 dNTP Mix,NEB,Cat# N0447S Aminoallyl-dUTP,ThermoFisher,Cat#

(15) HybriSeq: probe-based device-free single-cell RNA profilingCommun BiolAugust 19, 2025Daniel Foyt, David Brown, Shuqin Zhou, Brittany Moser, Qin Zhu, Zev J. Gartner, Bo Huang0.4 mM ATP, 40 U/mL RNasin) and then resuspended in ligation buffer plus 2 µM SplintR Ligase (NEB ref M0375S). Cells were incubated for 1 h at 37 °C with gentle agitation. RNA Isolation Suspended cells were

(16) Graft-seq precisely maps RNA modifications via site-specific chemical grafting strategyCell Rep MethodsJuly 21, 2025Ting Li, Ke Ding, Ziming Bao, Quan Ma, Chenyang Huang, Jie Cao, Xiao Shu, Minsong Gao, Zisheng Luo, Xushen Xiong, et al.L-ascorbate,Energy Chemical,Cat# E082526 Sodium pyruvate,Gibco,Cat# 11360 SplintR ligase,New England Biolabs,Cat# M0375S SuperScript II Reverse Transcriptase,Invitrogen,Cat# 18064071 T4 Polynucleotide Kinase; T4 PNK,New England Biolabs

(17) Attomolar Detection of HIV-1 with Label-Free RCA-rCRISPR on SmartphonebioRxivJune 19, 2025Noor Mohammad, Anastasiia Steksova, Yuyang Tang, Leyao Huang, Alireza Velayati, Shengwei Zhang, Aditi Dey Poonam, Sina Jamalzadegan, Matthew Breen, Guochun Jiang, et al.various concentrations of RNA target in 1x SplintR buffer; B: 400 units/mL SplintR ligase (catalog# M0375S), 125 units/mL phi29 polymerase (catalog# M0269S), 0.5 mM dNTPs, and 250 ng/uL recombinant albumin in

(18) Visualizing the transcription and replication of influenza A viral RNAs in cells by multiple direct RNA padlock probing and in situ sequencing (mudRapp-seq)Nucleic Acids ResJune 9, 2025Shazeb Ahmad, Jianhui Li, Joél Schaust, Anne-Sophie Gribling-Burrer, Nina Geiger, Sabine C Fischer, Uddhav B Ambi, Simone Backes, Markus J Ankenbrand, Redmond P Smythsplint-R ligase with a ligation mix containing 1× splint-R ligase buffer and 210 nM splint-R ligase [M0375S, New England Biolabs (NEB)] for 1 h at RT. After ligation, samples were washed three times with 1× PBS-T

(19) N(6)-methyladenosine in 7SK small nuclear RNA underlies RNA polymerase II transcription regulationMolecular cellNovember 2, 2023Wang Y, Traugot CM, Bubenik JL, Li T, Sheng P, Hiers NM, Fernandez P, Li L, Bian J, Swanson MS, et al.60°C, 50°C for 1 min and 40°C for 6 min. 2.5 μL of enzyme mixture including 0.5 U SplintR ligase (NEB, M0375S), 0.01 U Bst 2.0 DNA polymerase (NEB, M0537S) and 10 nM ATP (NEB, P0756S) were added and the reaction

(20) Specific Regulation of m(6)A by SRSF7 Promotes the Progression of GlioblastomaGenomics, proteomics & bioinformaticsAugust 1, 2023Cun Y, An S, Zheng H, Lan J, Chen W, Luo W, Yao C, Li X, Huang X, Sun X, et al.70 °C, 1 min; 60 °C, 1 min; 50 °C, 1 min; and 40 °C, 6 min. Then 0.5 U SplintR ligase (Catalog No. M0375S, NEB), 0.01 U Bst 2.0 DNA polymerase (Catalog No. M0537S, NEB), and 10 nM ATP (Catalog No. P0756L, NEB)

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SplintR Ligase

SplintR Ligase from New England Biolabs

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New England Biolabs

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SplintR Ligase from New England Biolabs

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