Esp3I R0734L from New England Biolabs

Product Specs
ItemEsp3I
CompanyNew England Biolabs
Price $373.00 Inquire
Catalog NumberR0734L
Size1500 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) SAGA/ATAC complexes sustain aberrant chromatin regulation and promote tumorigenesis in diffuse midline gliomabioRxivFebruary 6, 2026Rosemary U. Richard, Caitlin Bagnetto, Rebecca L. Murdaugh, Brittany R. Eberl, Alan L. Jiao, Adam F. Kebede, Barry M. Zee, Margaret M. Harrington, Mariella G. Filbin, Akdes Serin-Harmanci, et al.cloned into pLenti SpBsmBI sgRNA hygro (cat# 62205, AddGene) that was first digested by Esp3I (cat# R0734L, NEB) using T4 DNA ligase (cat# M0202L, NEB). Plasmid products were cleaned using the NEB Monarch DNA gel extraction kit

(2) Image-based, pooled phenotyping reveals multidimensional, disease-specific variant effectsbioRxivJuly 5, 2025Sriram Pendyala, Katie Partington, Nicholas Bradley, Abbye E. McEwen, Gwenneth Straub, Hyeon-Jin Kim, Shawn Fayer, Daniel Lee Holmes, Katherine A. Sitko, Allyssa J. Vandi, et al.M0202M) BsaI-HF-v2 (New England Biolabs, R3733L) SapI (New England Biolabs, R0569L) Esp3I (New England Biolabs, R0734L) BsmBI-v2 (New England Biolabs, R0739S) NheI-HF (New England Biolabs, R3131L) MluI-HF (New England Biolabs,

(3) Toward optimizing diversifying base editors for high-throughput mutational scanning studiesNucleic Acids ResJuly 4, 2025Carley I Schwartz, Nathan S Abell, Amy Li, Aradhana, Josh Tycko, Alisa Truong, Stephen B Montgomery, Gaelen T Hess100 μl restriction digest of pGH643 was incubated overnight at 37°C [10 μg pGH643, 4.5 μl Esp3I (NEB, R0734L), 4.5 μl BlpI (NEB, R0585L), and 10 μl of CutSmart Buffer (NEB)]. The digest reaction was run on a 1%

(4) Characterizing protein sequence determinants of nuclear condensates by high-throughput pooled imaging with CondenSeqNature MethodsJuly 1, 2025Kalli Kappel, Daniel Strebinger, KeHuan K. Edmonds, Samuel Chau-Duy-Tam VO, Christopher M. Vockley, Tridib Biswas, Samouil L. Farhi, Rhiannon Macrae, Feng Zhang, Aviv Regevresistance cassette. First, the backbone plasmid was digested with Esp3I (New England Biolabs Cat. # R0734L) and gel extracted. The library was then cloned into this digested backbone plasmid in a 120 μL (large

(5) High-resolution profiling reveals coupled transcriptional and translational regulation of transgenesNucleic Acids ResJune 18, 2025Emma L Peterman, Deon S Ploessl, Kasey S Love, Valeria Sanabria, Rachel F Daniels, Christopher P Johnstone, Diya R Godavarti, Sneha R Kabaria, Conrad G Oakes, Athma A Pai, et al.paired nicking (ITPN) editing [29]. The PCR fragments were assembled into a pHarbor using an Esp3I (NEB, R0734L) Golden Gate reaction. The LP architecture inserted at Rogi2 was based on the STRAIGHT-IN platform [30,

(6) Selective targeting of genome amplifications and repeat elements by CRISPR-Cas9 nickases to promote cancer cell deathNat CommunJune 2, 2025Matthew B. Hanlon, Jason M. Shohet, Scot A. WolfeIVT vectors were constructed and subsequently digested overnight with Esp3I (New England Biolabs, #R0734L) to ensure complete linearization of the DNA template. Linearized template was subsequently purified

(7) Genetically Encoded Fluorescence Barcodes Allow for Single-Cell Analysis via Spectral Flow CytometryACS Synthetic BiologyMay 16, 2025Xiaoming Lu, Daniel J. Pritko, Megan E. Abravanel, Jonah R. Huggins, Oluwaferanmi Ogunleye, Tirthankar Biswas, Katia C. Ashy, Semaj K. Woods, Mariclaire W.T. Livingston, Mark A. Blenner, et al.insert and vector were purified and ligated by Goldengate assembly with Esp3I (New England Biolabs, Cat# R0734L) and T4 ligase (New England Biolabs, Cat# M0202L), following the manufacturer’s instructions. Construction

(8) A comprehensive map of missense trafficking variants in rhodopsin and their response to pharmacologic correctionbioRxivMarch 4, 2025Kannan V. Manian, Connor H. Ludwig, Yan Zhao, Nathan Abell, Xiaoping Yang, David E. Root, Matthew L. Albert, Jason Comanderthese donor vectors and the plasmid libraries from Step 1 cloning were pre-digested with Esp3I (NEB, R0734L) prior to their use in Golden Gate reactions with the BsmBI-v2 NEBridge® Golden Gate Assembly Kit (NEB,

(9) Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSERCell GenomicsFebruary 12, 2025Siyan Liu, Marisa C. Hamilton, Thomas Cowart, Alejandro Barrera, Lexi R. Bounds, Alexander C. Nelson, Sophie F. Dornbaum, Julia W. Riley, Richard W. Doty, Andrew S. Allen, et al.Qiagen,Qiagen,Cat#27106 SYBR Safe DNA Gel Stain,Invitrogen,Cat#S33102 Esp3I,New England Biolabs,Cat#R0734L 1 kKb Plus DNA Ladder,Invitrogen,Cat#10787026 UltraPure™ Agarose,Invitrogen,Cat#16500100 NEBNext Ultra II Q5 Master Mix

(10) Haplotype rather than single causal variants effects contribute to regulatory gene expression associations in human myeloid cellsbioRxivFebruary 1, 2025Emily Greenwood, Mingming Cao, Ciaran M. Lee, Aidi Liu, Buhle Moyo, Gang Bao, Greg Gibsonuse. The CROPseq-Guide-Puro plasmid24 (Addgene, 86708) was digested by restriction enzyme, Esp3I (NEB, R0734L) and gel-purified (Qiagen, 28706) to extract the backbone fragment (8,333 bp). Gibson assembly reactions

(11) Multiplex Expression Cassette Assembly: A flexible and versatile method for building complex genetic circuits in conventional vectorsPlant Biotechnology JournalDecember 1, 2024Xun Jiang, Zhuoxiang Zhang, Xiuming Wu, Changmei Li, Xuan Sun, Yiting Li, Aixia Chang, Aiguo Yang, Changqing YangμL T4 DNA ligase (New England Biolabs, Cat. no. M0202L), 0.5 μL Esp3I (New England Biolabs, Cat. no. R0734L) or BsaI (New England Biolabs, Cat. no. R3733L), or BpiI (Thermo Fisher Scientific, Cat. no. ER1012),

(12) Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screensNature BiotechnologyAugust 1, 2024Zihan Xu, Andras Sziraki, Jasper Lee, Wei Zhou, Junyue CaoBiolabs (NEB), M0201S) and were annealed. The CROP-seq-opti-Puro-T2A-GFP was digested by Esp3I (NEB, R0734L), and then the linearized backbone and the annealed duplex were ligated using the Blunt/TA Ligase Master Mix

(13) Injury-induced Foxm1 expression in the mouse kidney drives epithelial proliferation by a cyclin F-dependent mechanismJCI insightJune 25, 2024Noonan ML, Muto Y, Yoshimura Y, Leckie-Harre A, Wu H, Kalinichenko VV, Humphreys BD, Chang-Panesso M.dCas9-KRAB repression plasmid by Golden Gate Assembly with Esp3I restriction enzyme (New England Biolabs, R0734L) and T4 DNA ligase (New England Biolabs, M0202L) on a thermal cycler repeating 37°C for 5 minutes and

(14) Microbiota metabolism of intestinal amino acids impacts host nutrient homeostasis and physiologyCell Host & MicrobeMay 8, 2024Ting-Ting Li, Xi Chen, Da Huo, Mohammad Arifuzzaman, Shanshan Qiao, Wen-Bing Jin, Huiqing Shi, Xin V. Li, JRI Live Cell Bank Consortium, Iliyan D Iliev, et al.Extract,Fisher Bioreagents,Cat# BP1422-2 Erythromycin,VWR,Cat# 0219 Esp3I,NEW ENGLAND Biolabs,Cat# R0734L FeSO4·7H2O,ACROS ORGANICS,Cat# 423731000 Formic acid,Fisher Chemical,Cat# A117-50 Gentamicin,GOLDBIO,Cat#

(15) Cell surface RNAs control neutrophil recruitmentCellFebruary 15, 2024Ningning Zhang, Wenwen Tang, Lidiane Torres, Xujun Wang, Yasmeen Ajaj, Li Zhu, Yi Luan, Hongyue Zhou, Yadong Wang, Dingyao Zhang, et al.proteins","Chemicals, peptides, and recombinant proteins" ,, BsmBI,NEB,Cat# R0580 Esp3I,NEB,Cat# R0734L T4 DNA ligase,NEB,Cat# M0202L Ficoll Paque Plus,Cytiva,Cat# 17144002 Percoll,Cytiva,Cat# 17089101 DMSO,americanBio,Cat#

(16) Gain of chromosome 21 increases the propensity for P2RY8: :CRLF2 acute lymphoblastic leukemia via increased HMGN1 expressionFrontiers in OncologyJuly 6, 2023Elyse C. Page, Susan L. Heatley, Jacqueline Rehn, Paul Q. Thomas, David T. Yeung, Deborah L. White(Addgene #70182 and #70183) (27). The FgH1tUTG vector was digested with Esp3I [New England Biolabs (NEB) #R0734L] and rSAP (NEB #M0371L) for 1 h at 37°C. The complementary gRNAs were phosphorylated at a final concentration

(17) Novel Fas-TNFR chimeras that prevent Fas ligand-mediated kill and signal synergistically to enhance CAR T cell efficacyMolecular therapy. Nucleic acidsJune 13, 2023McKenzie C, El-Kholy M, Parekh F, Robson M, Lamb K, Allen C, Sillibourne J, Cordoba S, Thomas S, Pule M.using the QIAquick gel extraction kit (QIAGEN, 28706), and digested with Esp3I or BsaI-HF v.2 (NEB, R0734L and R3733L, respectively). Digested DNA fragments were purified using the QIAquick PCR purification

(18) Defining cellular complexity in human autosomal dominant polycystic kidney disease by multimodal single cell analysisNature communicationsOctober 30, 2022Muto Y, Dixon EE, Yoshimura Y, Wu H, Omachi K, Ledru N, Wilson PC, King AJ, Eric Olson N, Gunawan MG, et al.were annealed. Cloning with Golden gate assembly was performed with Esp3I restriction enzyme (NEB, R0734L) and T4 DNA ligase (NEB, M0202L) on a thermal cycler repeating 37 °C for 5 min and 16 °C for 5 min for

(19) Multimodal single cell sequencing implicates chromatin accessibility and genetic background in diabetic kidney disease progressionNature communicationsSeptember 6, 2022Wilson PC, Muto Y, Wu H, Karihaloo A, Waikar SS, Humphreys BD.annealed. Subsequently, cloning with Golden gate assembly was performed with Esp3I restriction enzyme (NEB, R0734L) and T4 DNA ligase (NEB, M0202L) on a thermal cycler repeating 37 °C for 5 min and 16 °C for 5 min for

(20) Nucleocapsid mutations R203K/G204R increase the infectivity, fitness, and virulence of SARS-CoV-2Cell Host & MicrobeDecember 8, 2021Haibo Wu et al.Assembly Kit (BsaI-HFv2),New England Biolabs,Cat#E1601L Esp3I restriction enzyme,New England Biolabs,Cat#R0734L Luciferase Assay System,Promega,Cat# E1501 ,, Experimental Models: Cell Lines,Experimental Models:

Be the First to Write a Review!

Esp3I

Esp3I from New England Biolabs

Description

New England Biolabs

Citations (23)

Reviews

Esp3I from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map