Recombinant Human TC25 Protein, HEK293 Lysate 230-10349-100 from RayBiotech

Description

Recombinant human Ras-related C3 botulinum toxin substrate 1 (TC25) protein overexpression cell lysate, derived from the transfected HEK293 cells. Purchase will also include one vial of normal control (Catalog #. 230-10006), the cell lysate of HEK293 cells transfected with empty expression vector

Product Specs
ItemRecombinant Human TC25 Protein, HEK293 Lysate
CompanyRayBiotech
Price $144.00Supplier Page View Company Product Page
Catalog Number230-10349-100
Size100 µg
ConcentrationDetermined by BCA protein assay
Conjugate/TagN-terminal his-tag
FormatLyophilized powder
PurityUnpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control
Molecular WeightRecombinant protein product has a calculated molecular mass of 23. The actual molecular weight may increase slightly due to the potential post-translational modifications (PTMs)
SpeciesHuman
Source/Expression SystemHEK293 cells
NCBI Full Gene NameRac family small GTPase 1
NCBI Gene AliasesMIG5, MRD48, Rac-1, TC-25, p21-Rac1
Molecule NameRas-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1
Research AreaCardiovascular Disease, Inflammation, Ras/Rap Signaling