Recombinant Human HDAC6 Protein HDAC6-313H from Creative BioMart | Biocompare.com

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Recombinant Human HDAC6 Protein

Description

Recombinant Human HDAC6 Protein is produced by HEK293 cells expression system

Product Specs
ItemRecombinant Human HDAC6 Protein
CompanyCreative BioMart
Price Supplier Page View Company Product Page
Catalog NumberHDAC6-313H
Purity≥90% by SDS-PAGE
Molecular Weight131 kDa
SpeciesHuman
Source/Expression SystemHEK293 cells
Gene NameHDAC6(Human)
Molecule NameHDAC6 histone deacetylase 6 [ Homo sapiens ]
NCBI Full Gene Namehistone deacetylase 6
NCBI Gene AliasesCPBHM, PPP1R90, JM21, HD6
ActivityDeacetylation activity was determined using GAK(Ac)-AMC, Boc-K(Ac)-AMC and Fluor-de-Lys (RHK-K(Ac)-AMC) substrates. Reactions were carried out in a 384-well plate in 20 µL of reaction buffer comprising of 50 mM HEPES, 140 mM NaCl, 10 mM KCl, 1 mg/ml bovine serum albumin (BSA), and 1 mM TCEP, pH 7.4, for 30 min at 37°C. The reaction was stopped by addition of 20 µl of trypsin solution (2 mg/ml trypsin) and a fluorescence signal of released AMC was quantified using a fluorometer. The enzyme kinetic values were calculated by non-linear regression analysis

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Creative BioMart

Creative BioMart

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