Recombinant Human HDAC5 Protein HDAC5-312H from Creative BioMart | Biocompare.com

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Recombinant Human HDAC5 Protein

Description

Recombinant Human HDAC5 Protein is produced by HEK293 cells expression system

Product Specs
ItemRecombinant Human HDAC5 Protein
CompanyCreative BioMart
Price Supplier Page View Company Product Page
Catalog NumberHDAC5-312H
Purity≥95% by SDS-PAGE
Molecular Weight45 kDa
SpeciesHuman
Source/Expression SystemHEK293 cells
Gene NameHDAC5(Human)
Molecule NameHDAC5 histone deacetylase 5 [ Homo sapiens ]
NCBI Full Gene Namehistone deacetylase 5
NCBI Gene AliasesHD5, NY-CO-9
ActivityDeacetylation activity was determined using the Boc-K(TFA)-AMC substrate. Reactions were carried out in a 384-well plate in 20 µL of reaction buffer comprising of 50 mM HEPES, 140 mM NaCl, 10 mM KCl, 1 mg/ml bovine serum albumin (BSA), and 1 mM TCEP, pH 7.4, for 30 min at 37°C. The reaction was stopped by addition of 20 µl of trypsin solution (2 mg/ml trypsin) and the fluorescence signal of released AMC was quantified using a fluorometer. The enzyme kinetic values were calculated by nonlinear regression analysis

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Creative BioMart

Creative BioMart

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