Recombinant Human TIAM1 Protein TIAM1-25H from Creative BioMart

Product Specs
ItemRecombinant Human TIAM1 Protein
CompanyCreative BioMart
Price Supplier Page View Company Product Page
Catalog NumberTIAM1-25H
PurityProtein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 4-20% polyacrylamide gradient gel. MBP-Tiam1 was determined to be 90% pure
Molecular Weight86 kDa
SpeciesHuman
Source/Expression SystemE. coli
Gene NameTIAM1(Human)
Molecule NameTIAM1
NCBI Full Gene NameTIAM Rac1 associated GEF 1
NCBI Gene AliasesTIAM-1
ActivityThe biological activity of Tiam1 DHPH can be determined from its ability to catalyze nucleotide exchange on Rac1 using the nucleotide exchange assay of Bodipy-GDP for excess GDP or GTP. Rac1 protein is pre-loaded with Bodipy-FL-GDP by adding excess EDTA e.g. 0.7 mmol EDTA per mmol Mg2+ ions present in the reaction. This sub-stock solution is then used in a dissociation assay format which indicates competition for exchange site with unlabeled nucleotide. The reaction is monitored by fluorescence measurement at 485nm Ex / 535nm Em. Stringent quality control ensures that the exchange rate of Bodipy-GDP or mant-GDP is enhanced at least five fold in the presence of 0.8 µM Tiam1 DHPH

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Recombinant Human TIAM1 Protein from Creative BioMart